A0PV84 (A0PV84_MYCUA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Name:	topA EMBL ABL06253.1
Ordered Locus Names:	MUL_4222

Organism

Mycobacterium ulcerans (strain Agy99) [Complete proteome] [HAMAP]

Taxonomic identifier

362242 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	934 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding RuleBase RU000518 SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733 RuleBase RU000518
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A0PV84 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 83D38D80E9CBB56C	FASTA	934	102,252
	10 20 30 40 50 60 MADPKTKGPG SRSSGGNGSG RRLVIVESPT KARKLAGYLG PRYIVESSRG HIRDLPRAAA 70 80 90 100 110 120 DVPAKFKSEP WARLGVNVDA DFEPLYIISP EKKSAVSELK DLLKDVDELY LATDGDREGE 130 140 150 160 170 180 AIAWHLLETL KPRVPVKRMV FHEITEPAIL EAAENPRDLD MDLVDAQETR RILDRLYGYE 190 200 210 220 230 240 VSPVLWKKVA PKLSAGRVQS VATRMIVQRE RDRMAFRRAS YWDIVATLDA SVSDPKAAPP 250 260 270 280 290 300 TFVARLTSVD GLRVATGRDF DSLGTLRKAD EVTVLDEARA AALATGLHGA QLSVASVEEK 310 320 330 340 350 360 PYSRRPYPPF MTSTLQQEAG RKLRFSGERT MSIAQRLYEN GYITYMRTDS TTLSESAINA 370 380 390 400 410 420 ARTQARQLYG EEYVASSPRQ YTRKVKNAQE AHEAIRPAGE TFATPDAVRR ELDGDEFRLY 430 440 450 460 470 480 ELIWQRTVAS QMADARGTTL SLWIGGSAGE QQVVFSASGR TLTFPGFLKA YVETVDELVG 490 500 510 520 530 540 GEADDAERRL PNLTPGQRLD AVELTPDGHT TNPLARYTEA SLVKAFEELG IGRPSTYTPI 550 560 570 580 590 600 IKTIQDRGYV HKKGSALVPS WVAFAVTGLL EQHFGHLVDY GFTAAMEDEL DAIASGQERR 610 620 630 640 650 660 TDWLNNFYFG GDHGVSDSVA RAGGLKKLVG VNLEGIDARE VNSIKLFDDE QGRPIYVRVG 670 680 690 700 710 720 KNGPYLERTI TGEDGEPTPQ RANLNDSLTP DELTLVLAEE LFATPQEGRV LGIDAESGHE 730 740 750 760 770 780 IVAKDGRYGP YVTEILPEPP APEAGTEAPA KKSKKATAPK PRSGSLLRSM DLQTITLEDA 790 800 810 820 830 840 LKLLSLPRVV GTDPASGEEI TAQNGRYGPY LKRGTDSRSL ATEDQIFSIT LDEALKIYAE 850 860 870 880 890 900 PKRSGRQSAS APPLRELGTD AASGKAMVIK DGRFGPYVTD GETNASLRKG DEVASITDER 910 920 930 ASELLADRRA RGPVKRAAKK TTRKSPAKKA VKRA

References

[1]

"Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer." Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M. , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T. Genome Res. 17:192-200(2007) [PubMed: 17210928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000325 Genomic DNA. Translation: ABL06253.1.
RefSeq	YP_907724.1. NC_008611.1.
3D structure databases
ProteinModelPortal	A0PV84.
ModBase	Search...
Protein-protein interaction databases
STRING	A0PV84.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000073637; EBMYCP00000071642; EBMYCG00000073632.
GeneID	4551233.
GenomeReviews	Gene locus MUL_4222 in contig CP000325_GR.
KEGG	mul:MUL_4222.
PATRIC	18176172. VBIMycUlc37413_4895.
Organism-specific databases
GenoList	MUL_4222.
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
GeneTree	EBGT00050000016895.
HOGENOM	HBG512935.
OMA	STEPVIG.
ProtClustDB	PRK07561.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A0PV84_MYCUA

Accession

Primary (citable) accession number: A0PV84

Entry history

Integrated into UniProtKB/TrEMBL:	January 9, 2007
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)