A0PUT6 (OTSA_MYCUA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trehalose-phosphate synthase
Short name=TPS
EC=2.4.1.-

Alternative name(s):
Trehalose-6-phosphate synthase

Gene names

Name:	otsA
Ordered Locus Names:	MUL_4052

Organism

Mycobacterium ulcerans (strain Agy99) [Complete proteome] [HAMAP]

Taxonomic identifier

362242 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6-phosphate and nucleoside diphosphate By similarity.
Catalytic activity	Nucleoside diphosphate-glucose + D-glucose 6-phosphate = trehalose 6-phosphate + nucleoside diphosphate.
Pathway	Glycan biosynthesis; trehalose biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the glycosyltransferase 20 family.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Trehalose-phosphate synthase		PRO_0000348924
Regions
	Region	48 – 49	2	NDP-glucose binding By similarity
	Region	403 – 407	5	NDP-glucose binding By similarity
Sites
	Binding site	28	1	Glucose-6-phosphate By similarity
	Binding site	104	1	Glucose-6-phosphate By similarity
	Binding site	158	1	Glucose-6-phosphate By similarity
	Binding site	300	1	NDP-glucose By similarity
	Binding site	305	1	NDP-glucose By similarity
	Binding site	338	1	Glucose-6-phosphate By similarity
	Site	113	1	Involved in alpha anomer selectivity By similarity
	Site	183	1	Involved in alpha anomer selectivity By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0PUT6 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 8F9B71DBA741915C	FASTA	500	56,100
	10 20 30 40 50 60 MGPGGRQSAE PASTEVFGDS DFVVVANRLP VDQERLPDGT IAWKRSPGGL VTALEPLLRR 70 80 90 100 110 120 RRGAWVGWAG VVENDVDVQD EPIVQDELQL QPVRLSADDV AQYYEGFSNA TLWPLYHDVI 130 140 150 160 170 180 VRPIYHREWW DRYVDVNRRF AEATARAAAR GGTVWVQDYQ LQLVPKMLRA MRPDLTIGFF 190 200 210 220 230 240 LHIPFPPVEL FMQLPWRTEI IQGLLGADLV GFHLPGGAQN FLILSRRLVG ADTSRGTVGV 250 260 270 280 290 300 RSRFGEVILG SRTIRVGAFP ISIDSGALDQ TARDRNIRRR SREIRAELGN PRKILLGVDR 310 320 330 340 350 360 LDYTKGIDVR LKAFSELLAE GRVKRDDTVL VQLATPSRER VESYQTLRND IERQVGHING 370 380 390 400 410 420 EYAEVGHPVV HYLHRPVPRN ELIAFFVASD VMLVTPLRDG MNLVAKEYVA CRSDLGGALV 430 440 450 460 470 480 LSEFTGAAAE LRHAYLVNPH DLEGVNDGIE EALNQTEEAG RRRMRSMRRQ VLAHDVDRWA 490 500 RSFLDALADS RPNDSADAAD

References

[1]

"Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer." Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M. , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T. Genome Res. 17:192-200(2007) [PubMed: 17210928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Agy99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000325 Genomic DNA. Translation: ABL06105.1.
RefSeq	YP_907576.1. NC_008611.1.
3D structure databases
ProteinModelPortal	A0PUT6.
ModBase	Search...
Protein-protein interaction databases
STRING	A0PUT6.
Protein family/group databases
CAZy	GT20. Glycosyltransferase Family 20.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000074813; EBMYCP00000072818; EBMYCG00000074808.
GeneID	4550106.
GenomeReviews	Gene locus MUL_4052 in contig CP000325_GR.
KEGG	mul:MUL_4052.
PATRIC	18175808. VBIMycUlc37413_4715.
Organism-specific databases
GenoList	MUL_4052.
CMR	Search...
Phylogenomic databases
eggNOG	COG0380.
GeneTree	EBGT00050000016525.
HOGENOM	HBG559076.
OMA	EYGRKEM.
PhylomeDB	A0PUT6.
ProtClustDB	CLSK872185.
Family and domain databases
InterPro	IPR001830. Glyco_trans_20. [Graphical view]
KO	K00697.
Pfam	PF00982. Glyco_transf_20. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTSA_MYCUA

Accession

Primary (citable) accession number: A0PUT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program