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A0PUI6 (GLGB_MYCUA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:MUL_3936
OrganismMycobacterium ulcerans (strain Agy99) [Complete proteome] [HAMAP]
Taxonomic identifier362242 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length731 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7317311,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_1000044986

Sites

Active site4111Nucleophile By similarity
Active site4641Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
A0PUI6 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: 250FF1C8AAE0FA0B

FASTA73181,531
        10         20         30         40         50         60 
MSRTDQLAGT HLAPEPNDLA LLVAGSHHNP HGVLGAHEYG DHIVIRAFRP HAAEVIAIVG 

        70         80         90        100        110        120 
DDRFPMQHIE SGLFAVALPF VDLIDYRLQV SYENSEPFTV ADAYRFLPTL GEVDLHLFAE 

       130        140        150        160        170        180 
GRHERLWEAL GAHPRSFTTA DGVVHGVSFA VWAPNAEGVS LIGDFNGWSG SEAPMRVLGS 

       190        200        210        220        230        240 
SGVWELFWPD FPADGLYKFR VHGADGVVSE RADPFAFGTE VPPQTASRVT VSDYSWGDGD 

       250        260        270        280        290        300 
WMTERAQRNP VFEPMSTYEV HLGSWRPGLS YRQLATELTD YVVAHKFTHV ELLPVAEHPF 

       310        320        330        340        350        360 
AGSWGYQVTS YYAPTSRFGT PDDFRALVDA LHQAGIGVIV DWVPAHFPKD AWALGRFDGT 

       370        380        390        400        410        420 
PLYEHSDPKR GEQLDWGTYV FDFGRPEVRN FLVANALYWI EQFHIDGLRV DAVASMLYLD 

       430        440        450        460        470        480 
YSRPQDGWTP NIYEGRENLE AVQFLQEMNA TAHKAAPGIV TIAEESTSWP GVTRPTSIGG 

       490        500        510        520        530        540 
LGFSMKWNMG WMHDTLDYVS RDPIYRSYHH HEMTFSMLYA FSENYVLPLS HDEVVHGKGT 

       550        560        570        580        590        600 
LWGRMPGSNH TKAAGLRSLL AYQWAHPGKQ LLFMGQEFGQ RAEWSEQRGL DWFQLDENGF 

       610        620        630        640        650        660 
SNGVLRLVRD INEIYCDHCA LWSQDTTPEG YSWIDANDSA NNVLSFLRHG SDGSVMACIF 

       670        680        690        700        710        720 
NFAGSEHSDY RLGLPIAGRW REVLNTDATI YNGSGVGNFG GVDATAEPWH GRPASAVLVL 

       730 
PPSSALWLEP A 

« Hide

References

[1]"Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer."
Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M. expand/collapse author list , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T.
Genome Res. 17:192-200(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Agy99.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000325 Genomic DNA. Translation: ABL06005.1.
RefSeqYP_907476.1. NC_008611.1.

3D structure databases

ProteinModelPortalA0PUI6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING362242.MUL_3936.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABL06005; ABL06005; MUL_3936.
GeneID4552503.
KEGGmul:MUL_3936.
PATRIC18175544. VBIMycUlc37413_4587.

Organism-specific databases

GenoListMUL_3936.
CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAISEGRHE.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_MYCUA
AccessionPrimary (citable) accession number: A0PUI6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: June 11, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries