A0PUI6 (GLGB_MYCUA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE

Gene names

Name:	glgB
Ordered Locus Names:	MUL_3936

Organism

Mycobacterium ulcerans (strain Agy99) [Complete proteome] [HAMAP]

Taxonomic identifier

362242 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium ulcerans

Protein attributes

Sequence length	731 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-HAMAP
Molecular_function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: UniProtKB-HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 731	731	1,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685		PRO_1000044986
Sites
	Active site	411	1	Nucleophile By similarity
	Active site	464	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0PUI6 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 250FF1C8AAE0FA0B	FASTA	731	81,531
	10 20 30 40 50 60 MSRTDQLAGT HLAPEPNDLA LLVAGSHHNP HGVLGAHEYG DHIVIRAFRP HAAEVIAIVG 70 80 90 100 110 120 DDRFPMQHIE SGLFAVALPF VDLIDYRLQV SYENSEPFTV ADAYRFLPTL GEVDLHLFAE 130 140 150 160 170 180 GRHERLWEAL GAHPRSFTTA DGVVHGVSFA VWAPNAEGVS LIGDFNGWSG SEAPMRVLGS 190 200 210 220 230 240 SGVWELFWPD FPADGLYKFR VHGADGVVSE RADPFAFGTE VPPQTASRVT VSDYSWGDGD 250 260 270 280 290 300 WMTERAQRNP VFEPMSTYEV HLGSWRPGLS YRQLATELTD YVVAHKFTHV ELLPVAEHPF 310 320 330 340 350 360 AGSWGYQVTS YYAPTSRFGT PDDFRALVDA LHQAGIGVIV DWVPAHFPKD AWALGRFDGT 370 380 390 400 410 420 PLYEHSDPKR GEQLDWGTYV FDFGRPEVRN FLVANALYWI EQFHIDGLRV DAVASMLYLD 430 440 450 460 470 480 YSRPQDGWTP NIYEGRENLE AVQFLQEMNA TAHKAAPGIV TIAEESTSWP GVTRPTSIGG 490 500 510 520 530 540 LGFSMKWNMG WMHDTLDYVS RDPIYRSYHH HEMTFSMLYA FSENYVLPLS HDEVVHGKGT 550 560 570 580 590 600 LWGRMPGSNH TKAAGLRSLL AYQWAHPGKQ LLFMGQEFGQ RAEWSEQRGL DWFQLDENGF 610 620 630 640 650 660 SNGVLRLVRD INEIYCDHCA LWSQDTTPEG YSWIDANDSA NNVLSFLRHG SDGSVMACIF 670 680 690 700 710 720 NFAGSEHSDY RLGLPIAGRW REVLNTDATI YNGSGVGNFG GVDATAEPWH GRPASAVLVL 730 PPSSALWLEP A

References

[1]

"Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer." Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M. , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T. Genome Res. 17:192-200(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Agy99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000325 Genomic DNA. Translation: ABL06005.1.
RefSeq	YP_907476.1. NC_008611.1.
3D structure databases
ProteinModelPortal	A0PUI6.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	362242.MUL_3936.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABL06005; ABL06005; MUL_3936.
GeneID	4552503.
KEGG	mul:MUL_3936.
PATRIC	18175544. VBIMycUlc37413_4587.
Organism-specific databases
GenoList	MUL_3936.
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HOG000283037.
KO	K00700.
OMA	ISEGRHE.
OrthoDB	EOG6JX7GT.
Enzyme and pathway databases
UniPathway	UPA00164.
Family and domain databases
Gene3D	2.60.40.10. 2 hits. 2.60.40.1180. 1 hit. 3.20.20.80. 1 hit.
HAMAP	MF_00685. GlgB.
InterPro	IPR006048. A-amylase_b_C. IPR006407. GlgB. IPR015902. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. SSF51445. 1 hit. SSF81296. SSF81296. 2 hits.
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_MYCUA

Accession

Primary (citable) accession number: A0PUI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	June 11, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program