A0PUG0 (RNPH_MYCUA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	MUL_3908

Organism

Mycobacterium ulcerans (strain Agy99) [Complete proteome] [HAMAP]

Taxonomic identifier

362242 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tRNA binding Inferred from electronic annotation. Source: InterPro tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: EC tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	Ribonuclease PH HAMAP MF_00564		PRO_1000024835

Sequences

	Sequence		Length	Mass (Da)
	A0PUG0 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 0EB1621B16DC66E1	FASTA	259	27,190
	10 20 30 40 50 60 MSKREDGRRD DELRPVVITR GFTENPAGSV LIEFGRTKVM CTASVTEGVS RWRKGSGLGW 70 80 90 100 110 120 LTAEYAMLPS ATHTRSDRES VKGRVGGRTQ EISRLVGRSL RACIDLAALG ENTIAVDCDV 130 140 150 160 170 180 LQADGGTRTA AITGAYVALA DAVNYLAAAG KLSDPRPLSC AIAAVSVGVV DGRVRVDLPY 190 200 210 220 230 240 EEDSRAEVDM NVVATDTGTL VEVQGTGEGA TFPRSTLDKL LDMALGACDT LFAAQCDALA 250 LPYPGVLPEG PPPSKAFGS

References

[1]

"Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer." Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M. , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T. Genome Res. 17:192-200(2007) [PubMed: 17210928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Agy99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000325 Genomic DNA. Translation: ABL05979.1.
RefSeq	YP_907450.1. NC_008611.1.
3D structure databases
ProteinModelPortal	A0PUG0.
SMR	A0PUG0. Positions 3-248.
ModBase	Search...
Protein-protein interaction databases
STRING	A0PUG0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000074755; EBMYCP00000072760; EBMYCG00000074750.
GeneID	4553724.
GenomeReviews	Gene locus MUL_3908 in contig CP000325_GR.
KEGG	mul:MUL_3908.
PATRIC	18175488. VBIMycUlc37413_4559.
Organism-specific databases
GenoList	MUL_3908.
CMR	Search...
Phylogenomic databases
eggNOG	COG0689.
GeneTree	EBGT00050000017525.
HOGENOM	HBG737187.
OMA	MLPRATG.
PhylomeDB	A0PUG0.
ProtClustDB	PRK00173.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
KO	K00989.
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_MYCUA

Accession

Primary (citable) accession number: A0PUG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program