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Protein

Probable DNA ligase

Gene

lig

Organism
Mycobacterium ulcerans (strain Agy99)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei215 – 2151ATPUniRule annotation
Active sitei217 – 2171N6-AMP-lysine intermediateUniRule annotation
Binding sitei222 – 2221ATPUniRule annotation
Binding sitei237 – 2371ATPUniRule annotation
Binding sitei266 – 2661ATPUniRule annotation
Binding sitei378 – 3781ATPUniRule annotation
Binding sitei384 – 3841ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. DNA biosynthetic process Source: InterPro
  4. DNA ligation involved in DNA repair Source: InterPro
  5. DNA recombination Source: UniProtKB-HAMAP
  6. DNA replication Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:MUL_3413
OrganismiMycobacterium ulcerans (strain Agy99)
Taxonomic identifieri362242 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000765: Chromosome

Organism-specific databases

GenoListiMUL_3413.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 513513Probable DNA ligasePRO_0000365233Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi362242.MUL_3413.

Structurei

3D structure databases

ProteinModelPortaliA0PTC0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1793.
HOGENOMiHOG000036008.
KOiK01971.
OMAiGRPRPFQ.
OrthoDBiEOG6Q8HXT.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0PTC0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLLIDIASTS LNVGGTSSRL AKVARIAEPL RAAAPDPELV AIVVAWLSGE
60 70 80 90 100
LRQRQIGVGW ATLRSPPAAA GSPALTVTGT DAAFSEIGAV SGKGSAARRR
110 120 130 140 150
ELITRLFAAS TETEQAFLVR LLSGELRQGA LAGIMVDAVA RAAEVPATAV
160 170 180 190 200
QRAAMLGGDL PTVAAACLAA GSSGAAGALD SFTLRVGRPI GPMLAQSAGS
210 220 230 240 250
ITDALERHGG ATIFEAKLDG ARVQIHRTGD EVTVYTRSLD DVTARLPEVV
260 270 280 290 300
QATLALPVSD LVADGEAIAL QPDGRPHRFQ VTASRFGRSV NVAAAQAKQP
310 320 330 340 350
LSVFFSDILH RDGRDLLDAP TTDRLAALDA VVPARHRVDR LTTADAAAAT
360 370 380 390 400
DFLRATLAAG HEGVMAKSPT APYLAGRRGA GWLKVKPVHT LDLVVLAVEW
410 420 430 440 450
GSGRRRGKLS NIPLGARDAA TGEFVMLGKT FKSMTDAMLD WQTARFTELA
460 470 480 490 500
VGGTDGYVVA LRPEQVVEVA FDGVQASSRY PGGLALRFAR VVRYRDDKGP
510
ADADTIDTVR ALY
Length:513
Mass (Da):53,686
Last modified:January 9, 2007 - v1
Checksum:iE9004DEE06F58F4C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000325 Genomic DNA. Translation: ABL05589.1.
RefSeqiYP_907060.1. NC_008611.1.

Genome annotation databases

EnsemblBacteriaiABL05589; ABL05589; MUL_3413.
GeneIDi4554091.
KEGGimul:MUL_3413.
PATRICi18174281. VBIMycUlc37413_3969.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000325 Genomic DNA. Translation: ABL05589.1.
RefSeqiYP_907060.1. NC_008611.1.

3D structure databases

ProteinModelPortaliA0PTC0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi362242.MUL_3413.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL05589; ABL05589; MUL_3413.
GeneIDi4554091.
KEGGimul:MUL_3413.
PATRICi18174281. VBIMycUlc37413_3969.

Organism-specific databases

GenoListiMUL_3413.

Phylogenomic databases

eggNOGiCOG1793.
HOGENOMiHOG000036008.
KOiK01971.
OMAiGRPRPFQ.
OrthoDBiEOG6Q8HXT.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer."
    Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M.
    , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T.
    Genome Res. 17:192-200(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Agy99.

Entry informationi

Entry nameiDNLI_MYCUA
AccessioniPrimary (citable) accession number: A0PTC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: January 9, 2007
Last modified: February 4, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.