A0PT40 (DXS_MYCUA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	MUL_3319

Organism

Mycobacterium ulcerans (strain Agy99) [Complete proteome] [HAMAP]

Taxonomic identifier

362242 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	637 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 637	637	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000019046

Sequences

	Sequence		Length	Mass (Da)
	A0PT40 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: A455D63801648DE4	FASTA	637	67,830
	10 20 30 40 50 60 MLQQIRGPAD LQHLSQAQLR ELAQEIREFL VHKVAATGGH LGPNLGVVEL TLALHRVFDS 70 80 90 100 110 120 PHDPIIFDTG HQTYVHKMLT GRAQDFESLR KKGGLSGYPC RAESEHDWVE SSHASAALSY 130 140 150 160 170 180 ADGLSKAFEL SGLRNRHVVA VVGDGALTGG MCWEALNNIA ASHRPVVIVV NDNGRSYAPT 190 200 210 220 230 240 IGGVADHLAK LRLQPAYEQV LEKGRDVVRA VPLVGEVCYH FMHSVKAGIK DSLSPQLLFT 250 260 270 280 290 300 DLGLKYVGPV DGHDERAVEV ALRSARGFGG PVIVHVVTRK GMGYGPAEAD VAEQMHSTVP 310 320 330 340 350 360 IDPATGQATK LAGPGWTTTF ADALIEYAGK RRDIVAITAA MPGPTGLTAF GQQFPDRLFD 370 380 390 400 410 420 VGIAEQHAMT SAAGLAMGGM HPVVAIYSTF LNRAFDQIMM DVALHQLPVT MVLDRAGITG 430 440 450 460 470 480 SDGPSHNGMW DLSMLGIIPG IRVAAPRDAT RLREELGEAL DVDDGPTALR FPKGDVGEDI 490 500 510 520 530 540 PALERRDGMD VLAVPASGLN HDVLLIAVGA LASMALAVAK RLHNQGIGVT VIDPRWVLPV 550 560 570 580 590 600 ADGIGDLAVA HKLVVTLEDN GVNGGVGSAV SAALRRAEIG VHCRDVGLPQ QFFEHASRGE 610 620 630 LLADLRLTDQ DVARRVTGWV AALGACVSEA EIKEHLD

References

[1]

"Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer." Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M. , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T. Genome Res. 17:192-200(2007) [PubMed: 17210928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Agy99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000325 Genomic DNA. Translation: ABL05509.1.
RefSeq	YP_906980.1. NC_008611.1.
3D structure databases
ProteinModelPortal	A0PT40.
ModBase	Search...
Protein-protein interaction databases
STRING	A0PT40.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000074979; EBMYCP00000072984; EBMYCG00000074974.
GeneID	4554445.
GenomeReviews	Gene locus MUL_3319 in contig CP000325_GR.
KEGG	mul:MUL_3319.
PATRIC	18174068. VBIMycUlc37413_3861.
Organism-specific databases
GenoList	MUL_3319.
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
GeneTree	EBGT00050000016269.
HOGENOM	HBG571647.
OMA	QYDISYM.
PhylomeDB	A0PT40.
ProtClustDB	PRK05444.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_MYCUA

Accession

Primary (citable) accession number: A0PT40

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program