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Protein

Malate synthase G

Gene

glcB

Organism
Mycobacterium ulcerans (strain Agy99)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei275Acetyl-CoAUniRule annotation1
Binding sitei312Acetyl-CoAUniRule annotation1
Active sitei339Proton acceptorUniRule annotation1
Binding sitei339GlyoxylateUniRule annotation1
Metal bindingi438MagnesiumUniRule annotation1
Binding sitei438GlyoxylateUniRule annotation1
Metal bindingi466MagnesiumUniRule annotation1
Binding sitei547Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei637Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:MUL_3055
OrganismiMycobacterium ulcerans (strain Agy99)
Taxonomic identifieri362242 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000765 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000569111 – 731Malate synthase GAdd BLAST731

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei623Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA0PSI5
SMRiA0PSI5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 126Acetyl-CoA bindingUniRule annotation2
Regioni463 – 466Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000220740
KOiK01638
OMAiTEPVLHA
OrthoDBiPOG091H05P0

Family and domain databases

CDDicd00728 malate_synt_G, 1 hit
Gene3Di2.170.170.11, 1 hit
HAMAPiMF_00641 Malate_synth_G, 1 hit
InterProiView protein in InterPro
IPR011076 Malate_synth-like_sf
IPR023310 Malate_synth_G_beta_sub_dom
IPR001465 Malate_synthase
IPR006253 Malate_synthG
PANTHERiPTHR42739 PTHR42739, 1 hit
PfamiView protein in Pfam
PF01274 Malate_synthase, 1 hit
SUPFAMiSSF51645 SSF51645, 1 hit
TIGRFAMsiTIGR01345 malate_syn_G, 1 hit

Sequencei

Sequence statusi: Complete.

A0PSI5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDRVSAGNL RVARVLYDFV NNEALPGTDI DQDSFWAGVD KVVTDLTPQN
60 70 80 90 100
QDLLKTRDDL QAQIDKWHRH RVIEPLDPQA YREFLTEIGY LLPAPEDFTI
110 120 130 140 150
TTSGVDDEIT TTAGPQLVVP ILNARFALNA ANARWGSLYD ALYGTDVISE
160 170 180 190 200
SDGAEKGRGY NKVRGDKVIA YARQFLDDSV PLAGASYTDA TGFKVEDGQL
210 220 230 240 250
VVSLADTSAA LADPGQFAGY TGTAENPKSI LLANHGLHIE ILIDPESQIG
260 270 280 290 300
ATDGAGVKDV ILESAITTIM DFEDSVAAVD ADDKALGYRN WLGLNRGDLS
310 320 330 340 350
EDVTKDDKTF TRVLNTDRTY TAPHGGELTL PGRSLLFVRN VGHLMTNDAI
360 370 380 390 400
VSDAEGAEGA PVFEGIMDAL FTGLIAIHGL RSTDANGLLT NSRTGSIYIV
410 420 430 440 450
KPKMHGPAEV AFTCELFSRV EDVLGLPQGT MKVGIMDEER RTTLNLKACI
460 470 480 490 500
KAAADRVVFI NTGFLDRTGD EIHTSMEAGP MIRKGAMKNT AWIKAYEDAN
510 520 530 540 550
VDTGLAAGFS GKAQIGKGMW AMTELMADMV EQKIAQPKAG ATTAWVPSPT
560 570 580 590 600
AATLHAMHYH KVDVFAVQKE LQGKTRTSVD ELLTIPLAKE LAWAPEEIRE
610 620 630 640 650
EVDNNCQSIL GYVVRWIDQG VGCSKVPDIH NVALMEDRAT LRISSQLLAN
660 670 680 690 700
WLRHGVITSE DVRASLERMA PLVDKQNAGD PEYHAMAPNF DDSIAFLAAQ
710 720 730
DLILSGAQQP NGYTEPILHR RRRELKARAG A
Length:731
Mass (Da):79,479
Last modified:January 9, 2007 - v1
Checksum:iBB2A4320CAA7264F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000325 Genomic DNA Translation: ABL05304.1
RefSeqiWP_011740916.1, NC_008611.1

Genome annotation databases

EnsemblBacteriaiABL05304; ABL05304; MUL_3055
KEGGimul:MUL_3055

Similar proteinsi

Entry informationi

Entry nameiMASZ_MYCUA
AccessioniPrimary (citable) accession number: A0PSI5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: April 25, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health