Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A0PSI5

- MASZ_MYCUA

UniProt

A0PSI5 - MASZ_MYCUA

Protein

Malate synthase G

Gene

glcB

Organism
Mycobacterium ulcerans (strain Agy99)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (09 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei275 – 2751Acetyl-CoAUniRule annotation
    Binding sitei312 – 3121Acetyl-CoAUniRule annotation
    Active sitei339 – 3391Proton acceptorUniRule annotation
    Binding sitei339 – 3391GlyoxylateUniRule annotation
    Metal bindingi438 – 4381MagnesiumUniRule annotation
    Binding sitei438 – 4381GlyoxylateUniRule annotation
    Metal bindingi466 – 4661MagnesiumUniRule annotation
    Binding sitei547 – 5471Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei637 – 6371Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:MUL_3055
    OrganismiMycobacterium ulcerans (strain Agy99)
    Taxonomic identifieri362242 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000765: Chromosome

    Organism-specific databases

    GenoListiMUL_3055.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 731731Malate synthase GPRO_1000056911Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei623 – 6231Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi362242.MUL_3055.

    Structurei

    3D structure databases

    ProteinModelPortaliA0PSI5.
    SMRiA0PSI5. Positions 5-730.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
    Regioni463 – 4664Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A0PSI5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDRVSAGNL RVARVLYDFV NNEALPGTDI DQDSFWAGVD KVVTDLTPQN    50
    QDLLKTRDDL QAQIDKWHRH RVIEPLDPQA YREFLTEIGY LLPAPEDFTI 100
    TTSGVDDEIT TTAGPQLVVP ILNARFALNA ANARWGSLYD ALYGTDVISE 150
    SDGAEKGRGY NKVRGDKVIA YARQFLDDSV PLAGASYTDA TGFKVEDGQL 200
    VVSLADTSAA LADPGQFAGY TGTAENPKSI LLANHGLHIE ILIDPESQIG 250
    ATDGAGVKDV ILESAITTIM DFEDSVAAVD ADDKALGYRN WLGLNRGDLS 300
    EDVTKDDKTF TRVLNTDRTY TAPHGGELTL PGRSLLFVRN VGHLMTNDAI 350
    VSDAEGAEGA PVFEGIMDAL FTGLIAIHGL RSTDANGLLT NSRTGSIYIV 400
    KPKMHGPAEV AFTCELFSRV EDVLGLPQGT MKVGIMDEER RTTLNLKACI 450
    KAAADRVVFI NTGFLDRTGD EIHTSMEAGP MIRKGAMKNT AWIKAYEDAN 500
    VDTGLAAGFS GKAQIGKGMW AMTELMADMV EQKIAQPKAG ATTAWVPSPT 550
    AATLHAMHYH KVDVFAVQKE LQGKTRTSVD ELLTIPLAKE LAWAPEEIRE 600
    EVDNNCQSIL GYVVRWIDQG VGCSKVPDIH NVALMEDRAT LRISSQLLAN 650
    WLRHGVITSE DVRASLERMA PLVDKQNAGD PEYHAMAPNF DDSIAFLAAQ 700
    DLILSGAQQP NGYTEPILHR RRRELKARAG A 731
    Length:731
    Mass (Da):79,479
    Last modified:January 9, 2007 - v1
    Checksum:iBB2A4320CAA7264F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000325 Genomic DNA. Translation: ABL05304.1.
    RefSeqiYP_906775.1. NC_008611.1.

    Genome annotation databases

    EnsemblBacteriaiABL05304; ABL05304; MUL_3055.
    GeneIDi4553683.
    KEGGimul:MUL_3055.
    PATRICi18173441. VBIMycUlc37413_3556.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000325 Genomic DNA. Translation: ABL05304.1 .
    RefSeqi YP_906775.1. NC_008611.1.

    3D structure databases

    ProteinModelPortali A0PSI5.
    SMRi A0PSI5. Positions 5-730.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 362242.MUL_3055.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABL05304 ; ABL05304 ; MUL_3055 .
    GeneIDi 4553683.
    KEGGi mul:MUL_3055.
    PATRICi 18173441. VBIMycUlc37413_3556.

    Organism-specific databases

    GenoListi MUL_3055.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer."
      Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M.
      , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T.
      Genome Res. 17:192-200(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Agy99.

    Entry informationi

    Entry nameiMASZ_MYCUA
    AccessioniPrimary (citable) accession number: A0PSI5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3