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Protein

Thiazole synthase

Gene

thiG

Organism
Mycobacterium ulcerans (strain Agy99)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei98 – 981Schiff-base intermediate with DXPUniRule annotation
Binding sitei159 – 1591DXP; via amide nitrogenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:MUL_2803
OrganismiMycobacterium ulcerans (strain Agy99)
Taxonomic identifieri362242 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000765 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 264264Thiazole synthasePRO_1000026015Add
BLAST

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA0PRX9.
SMRiA0PRX9. Positions 4-242.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni185 – 1862DXP bindingUniRule annotation
Regioni207 – 2082DXP bindingUniRule annotation

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000248049.
KOiK03149.
OMAiAQYPSPA.
OrthoDBiPOG091H01K9.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view]
SUPFAMiSSF110399. SSF110399. 1 hit.

Sequencei

Sequence statusi: Complete.

A0PRX9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVESKLMIAD RSFASRLIMG TGGASNLAVL QEALVASGTE LTTVAIRRVD
60 70 80 90 100
AEGGTGLLDL LNRLGITPLP NTAGCRSAAE AVLTAQLARE ALATNWVKLE
110 120 130 140 150
VIADERTLLP DAIELVRAAE QLVDDGFVVL PYTNDDPVLA RRLEDTGCAA
160 170 180 190 200
VMPLGSPIGT GLGITNPHNI EMIVASAGVP VVLDAGIGTA SDAALAMELG
210 220 230 240 250
CDALLLATAV TRAADPAAMA AAMSAAVTAG YLARCAGRIP KRFWAQASSP
260
TLVTTQSPGA ESGN
Length:264
Mass (Da):26,960
Last modified:January 9, 2007 - v1
Checksum:i930400A622E4FEB8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000325 Genomic DNA. Translation: ABL05098.1.

Genome annotation databases

EnsemblBacteriaiABL05098; ABL05098; MUL_2803.
KEGGimul:MUL_2803.
PATRICi18172868. VBIMycUlc37413_3271.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000325 Genomic DNA. Translation: ABL05098.1.

3D structure databases

ProteinModelPortaliA0PRX9.
SMRiA0PRX9. Positions 4-242.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL05098; ABL05098; MUL_2803.
KEGGimul:MUL_2803.
PATRICi18172868. VBIMycUlc37413_3271.

Phylogenomic databases

HOGENOMiHOG000248049.
KOiK03149.
OMAiAQYPSPA.
OrthoDBiPOG091H01K9.

Enzyme and pathway databases

UniPathwayiUPA00060.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view]
SUPFAMiSSF110399. SSF110399. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHIG_MYCUA
AccessioniPrimary (citable) accession number: A0PRX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: September 7, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.