A0PRD3 (AROA_MYCUA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	MUL_2560

Organism

Mycobacterium ulcerans (strain Agy99) [Complete proteome] [HAMAP]

Taxonomic identifier

362242 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_1000099728

Sequences

	Sequence		Length	Mass (Da)
	A0PRD3 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: FF4C5BDC06285D00	FASTA	431	44,593
	10 20 30 40 50 60 MSSIEPWPAP FAPTPVHATV TVPGSKSQTN RTLVLAALAA AQGQGSSTIT GALRSRDTDL 70 80 90 100 110 120 MIEALQTLGL RVDGTGSELT VSGRIRPCPE ARVDCGLAGT VLRFAPPLAA LSAAPITFDG 130 140 150 160 170 180 DEQARARPIA PLLDALRGLG VPVDGAGLPF RVQGTGSVAG GTVAIDASAS SQFVSGLLLS 190 200 210 220 230 240 GASFTDGLTV QHTGSELPSA PHIAMTVQML RQAGVDVDDS IPNRWLVRPG ALRPRHWDAE 250 260 270 280 290 300 PDLTNAVAFL AAAVVTGGTV TITGWPADSV QPAKNILDIL QTLNSTVRHI DSCLQVQGPQ 310 320 330 340 350 360 SYRGFDVDLR DVGELTPSVA ALAALASPGS VSRLAGIAHL RGHETDRLAA LSTEINRLGG 370 380 390 400 410 420 NCEQTSDGLV ITATPLRPGS WRAYADHRMA MAGAIVGLRV AGVEVDDIGA TSKTLPEFPQ 430 LWTEMVEGSS G

References

[1]

"Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer." Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M. , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T. Genome Res. 17:192-200(2007) [PubMed: 17210928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Agy99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000325 Genomic DNA. Translation: ABL04902.1.
RefSeq	YP_906373.1. NC_008611.1.
3D structure databases
ProteinModelPortal	A0PRD3.
SMR	A0PRD3. Positions 4-426.
ModBase	Search...
Protein-protein interaction databases
STRING	A0PRD3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000074394; EBMYCP00000072399; EBMYCG00000074389.
GeneID	4550049.
GenomeReviews	Gene locus MUL_2560 in contig CP000325_GR.
KEGG	mul:MUL_2560.
PATRIC	18172329. VBIMycUlc37413_3003.
Organism-specific databases
GenoList	MUL_2560.
CMR	Search...
Phylogenomic databases
eggNOG	COG0128.
GeneTree	EBGT00050000016231.
HOGENOM	HBG646626.
OMA	HRMATAG.
PhylomeDB	A0PRD3.
ProtClustDB	PRK02427.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_MYCUA

Accession

Primary (citable) accession number: A0PRD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program