A0PQG7 (TYSY_MYCUA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	MUL_2178

Organism

Mycobacterium ulcerans (strain Agy99) [Complete proteome] [HAMAP]

Taxonomic identifier

362242 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Thymidylate synthase HAMAP MF_00008		PRO_1000000635
Sites
	Active site	149	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0PQG7 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 52E95C0A55F29BDC	FASTA	266	30,094
	10 20 30 40 50 60 MPIATPYEDL LRRVLETGTA KSDRTGTGTR SLFGQQIRYD LGCGFPLLTT KKVHFKSVVY 70 80 90 100 110 120 ELLWFLRGDS NVGWLQQHGV TIWDEWASET GDLGPIYGVQ WRSWPTPSGE HIDQISAALE 130 140 150 160 170 180 LLRTDPDSRR IIVSAWNVGQ IPQMALPPCH AFFQFYVADG RLSCQLYQRS ADLFLGVPFN 190 200 210 220 230 240 IASYALLTHM MAAQAGLSVG EFIWTGGDCH IYDNHVEQVT EQLRREPRPY PKLSLSQRDS 250 260 IFDYTYEDVV VQDYDPHPAI KAPVAV

References

[1]

"Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer." Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M. , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T. Genome Res. 17:192-200(2007) [PubMed: 17210928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Agy99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000325 Genomic DNA. Translation: ABL04586.1.
RefSeq	YP_906057.1. NC_008611.1.
3D structure databases
ProteinModelPortal	A0PQG7.
SMR	A0PQG7. Positions 7-266.
ModBase	Search...
Protein-protein interaction databases
STRING	A0PQG7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000076890; EBMYCP00000074895; EBMYCG00000076885.
GeneID	4550647.
GenomeReviews	Gene locus MUL_2178 in contig CP000325_GR.
KEGG	mul:MUL_2178.
PATRIC	18171465. VBIMycUlc37413_2577.
Organism-specific databases
GenoList	MUL_2178.
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
GeneTree	EBGT00050000017116.
HOGENOM	HBG588098.
OMA	RMALAPC.
PhylomeDB	A0PQG7.
ProtClustDB	PRK01827.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KO	K00560.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. Thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_MYCUA

Accession

Primary (citable) accession number: A0PQG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program