A0PQB8 (SYP_MYCUA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	MUL_2116

Organism

Mycobacterium ulcerans (strain Agy99) [Complete proteome] [HAMAP]

Taxonomic identifier

362242 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	582 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 582	582	Proline--tRNA ligase HAMAP MF_01569		PRO_0000288353

Sequences

	Sequence		Length	Mass (Da)
	A0PQB8 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 5E6F3FE806045FF5	FASTA	582	63,570
	10 20 30 40 50 60 MITRMSELFL RTLRDDPADA EVPSHKLLIR AGYIRPVGPG LYSWLPLGLR VLRNIERVIR 70 80 90 100 110 120 EEMNAIGGQE ILFPALLPRA PYETTNRWTE YGDGVFRLED RRGNDYLLGP THEELFTLTV 130 140 150 160 170 180 KGEYNSYKDF PLTLYQIQIK YRDEARPRAG ILRAREFVMK DSYSFDIDSG GLKAAYHAHR 190 200 210 220 230 240 EAYQRIFDRL RVRYVIVSAV SGAMGGSASE EFLAESPVGE DTFVRCVESG YAANVEAVVT 250 260 270 280 290 300 ARPESLPIDG QPDAVVHDTG ETPTIATLVA WANEAGLGRE VSAADTLKNV LMKIRQPGGE 310 320 330 340 350 360 WELLAIGVPG DREIDEKRLA AALDPAEYVF LDDDDFGKYP FLVKGYIGPK ALRSNDVRYL 370 380 390 400 410 420 LDPRVVDGTS WITGADEPGR HVVGLVAGRD FTADGTIEAA EVREGDPAPD GAGQLVMARG 430 440 450 460 470 480 IEIGHIFQLG RKYTDAFTAD VLGEDGKPVR LTMGSYGIGV SRLVAVIAEQ HHDNLGLRWP 490 500 510 520 530 540 AEIAPFGVHL VIANKDAEAR AGAIGLAGEL DGLGVEVLLD DRQASPGVKF KDAELLGMPW 550 560 570 580 VVVVGRGWAD GVVELRDRFG GQTRELTVGD SLATDIAAAI SG

References

[1]

"Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer." Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M. , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T. Genome Res. 17:192-200(2007) [PubMed: 17210928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Agy99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000325 Genomic DNA. Translation: ABL04537.1.
RefSeq	YP_906008.1. NC_008611.1.
3D structure databases
ProteinModelPortal	A0PQB8.
ModBase	Search...
Protein-protein interaction databases
STRING	A0PQB8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000075345; EBMYCP00000073350; EBMYCG00000075340.
GeneID	4551000.
GenomeReviews	Gene locus MUL_2116 in contig CP000325_GR.
KEGG	mul:MUL_2116.
PATRIC	18171308. VBIMycUlc37413_2500.
Organism-specific databases
GenoList	MUL_2116.
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
GeneTree	EBGT00050000016802.
HOGENOM	HBG403504.
OMA	IQPAELW.
PhylomeDB	A0PQB8.
ProtClustDB	PRK09194.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.90.960.10. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMs	TIGR00409. ProS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_MYCUA

Accession

Primary (citable) accession number: A0PQB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	January 9, 2007
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program