A0PQ70 (RNH2_MYCUA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HII
Short name=RNase HII
EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	MUL_2054

Organism

Mycobacterium ulcerans (strain Agy99) [Complete proteome] [HAMAP]

Taxonomic identifier

362242 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP MF_00052_B
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052_B
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00052_B.
Sequence similarities	Belongs to the RNase HII family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	Ribonuclease HII HAMAP MF_00052_B		PRO_1000031169
Sites
	Metal binding	36	1	Divalent metal cation By similarity
	Metal binding	37	1	Divalent metal cation By similarity
	Metal binding	130	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0PQ70 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 2F40FAB2801AB0C6	FASTA	239	25,420
	10 20 30 40 50 60 MATTWPPRTV IRKSSGLRTL ESALQRSGLG PVAGVDEVGR GACAGPLVVA ACALGPNRYE 70 80 90 100 110 120 SLAALDDSKK LTEKTREKLF PLICRYALAY HVVFIPSVEV DRRGVHVANI EGMRRAVAGL 130 140 150 160 170 180 SVRPGYVLSD GFRVPGLSVP SLPVIGGDAA AACIAAASVL AKVSRDRLMV AMDTQYPGYG 190 200 210 220 230 FAEHKGYSTR AHTLALTQLG PCPEHRRSFI NVRRVATRSN GAATAEREAD PPQERDGTG

References

[1]

"Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer." Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M. , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T. Genome Res. 17:192-200(2007) [PubMed: 17210928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Agy99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000325 Genomic DNA. Translation: ABL04489.1.
RefSeq	YP_905960.1. NC_008611.1.
3D structure databases
ProteinModelPortal	A0PQ70.
ModBase	Search...
Protein-protein interaction databases
STRING	A0PQ70.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000076560; EBMYCP00000074565; EBMYCG00000076555.
GeneID	4551068.
GenomeReviews	Gene locus MUL_2054 in contig CP000325_GR.
KEGG	mul:MUL_2054.
PATRIC	18171170. VBIMycUlc37413_2432.
Organism-specific databases
GenoList	MUL_2054.
CMR	Search...
Phylogenomic databases
eggNOG	COG0164.
GeneTree	EBGT00050000016477.
HOGENOM	HBG584843.
OMA	RLGPTPI.
PhylomeDB	A0PQ70.
ProtClustDB	PRK00015.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B. [Tree]
InterPro	IPR022898. RNase_HII. IPR001352. RNase_HII/HIII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K03470.
PANTHER	PTHR10954. RNase_HII/HIII. 1 hit.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNH2_MYCUA

Accession

Primary (citable) accession number: A0PQ70

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program