A0PQ66 (A0PQ66_MYCUA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605
EC=2.1.1.228 HAMAP MF_00605

Alternative name(s):
M1G-methyltransferase HAMAP MF_00605
tRNA [GM37] methyltransferase HAMAP MF_00605

Gene names

Name:	trmD HAMAP MF_00605 EMBL ABL04485.1
Ordered Locus Names:	MUL_2050

Organism

Mycobacterium ulcerans (strain Agy99) [Complete proteome] [HAMAP]

Taxonomic identifier

362242 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605 RuleBase RU003464
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605 S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. RuleBase RU003464
Subunit structure	Homodimer By similarity. HAMAP MF_00605 RuleBase RU003464
Subcellular location	Cytoplasm By similarity HAMAP MF_00605 RuleBase RU003464.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family. HAMAP MF_00605 RuleBase RU003464

Ontologies

Keywords
Biological process	tRNA processing HAMAP MF_00605 RuleBase RU003464
Cellular component	Cytoplasm HAMAP MF_00605 RuleBase RU003464
Ligand	S-adenosyl-L-methionine HAMAP MF_00605 RuleBase RU003464
Molecular function	Methyltransferase HAMAP MF_00605 RuleBase RU003464 EMBL ABL04485.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	133 – 138	6	S-adenosyl-L-methionine binding By similarity HAMAP MF_00605
Sites
	Binding site	109	1	S-adenosyl-L-methionine; via amide nitrogen By similarity HAMAP MF_00605

Sequences

	Sequence		Length	Mass (Da)
	A0PQ66 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: FF9A445B5F7DD880	FASTA	230	25,460
	10 20 30 40 50 60 MKIDVVTIFP AYLDPLRQSL PGRAIASGLV DLQVHDLRRW TYDVHHSVDD SPYGGGPGMV 70 80 90 100 110 120 MKAPVWGEAL DEICSAETLL VVPTPAGSLF DQATAQRWSA EQHLVFACGR YEGIDQRVAE 130 140 150 160 170 180 DSARRMRVEE VSIGDYVLPG GESAAVVMIE AVLRLLTGVL GNPASHQEDS FSPELDRLLE 190 200 210 220 230 GPSYTRPPSW RGLDVPEILL SGDHARIATW RREVSLRRTR ERRPDLAPPE

References

[1]

"Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer." Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M. , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T. Genome Res. 17:192-200(2007) [PubMed: 17210928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000325 Genomic DNA. Translation: ABL04485.1.
RefSeq	YP_905956.1. NC_008611.1.
3D structure databases
ProteinModelPortal	A0PQ66.
ModBase	Search...
Protein-protein interaction databases
STRING	A0PQ66.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000073680; EBMYCP00000071685; EBMYCG00000073675.
GeneID	4551064.
GenomeReviews	Gene locus MUL_2050 in contig CP000325_GR.
KEGG	mul:MUL_2050.
PATRIC	18171162. VBIMycUlc37413_2428.
Organism-specific databases
GenoList	MUL_2050.
CMR	Search...
Phylogenomic databases
eggNOG	COG0336.
GeneTree	EBGT00050000016420.
HOGENOM	HBG285805.
OMA	VCGRFEG.
ProtClustDB	PRK00026.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A0PQ66_MYCUA

Accession

Primary (citable) accession number: A0PQ66

Entry history

Integrated into UniProtKB/TrEMBL:	January 9, 2007
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)