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A0PQ03 (DDL_MYCUA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:MUL_1976
OrganismMycobacterium ulcerans (strain Agy99) [Complete proteome] [HAMAP]
Taxonomic identifier362242 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 373373D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000030471

Regions

Domain156 – 363208ATP-grasp
Nucleotide binding184 – 23956ATP By similarity

Sites

Metal binding3181Magnesium or manganese 1 By similarity
Metal binding3301Magnesium or manganese 1 By similarity
Metal binding3301Magnesium or manganese 2 By similarity
Metal binding3321Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A0PQ03 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: 8A6AF728571CCA02

FASTA37339,729
        10         20         30         40         50         60 
MSGSSRRGER LRVAVVFGGR SNEHAISCVS AGSILRNLDP RRFEVIAIGI TPQGSWVLTD 

        70         80         90        100        110        120 
GNPDALAITD RRLPEVSSQS GTELALPADP RWGGQLVSLP PGAGEVLASV DVVFPVLHGP 

       130        140        150        160        170        180 
YGEDGTIQGL LELAGVPYVG AGVLASAAGM DKEFTKKLWS AAGLPIGPHA VLRPSRQSLD 

       190        200        210        220        230        240 
REELQRLGLP AYVKPARGGS SIGVSRVSSF DELPAAIAAA RRHDPKVIVE AAINGRELEC 

       250        260        270        280        290        300 
GVLEFPDGRL EASTLGEIRV AGVRGREDGF YDFETKYLDD AAELDVPAKV EDDVADAVRQ 

       310        320        330        340        350        360 
LAIRAFEAID CQGLARVDFF LTDDGPVINE LNTMPGFTTI SMYPRMWVAS GVDYPTLLAT 

       370 
MVETALSRGI GLR 

« Hide

References

[1]"Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer."
Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M. expand/collapse author list , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T.
Genome Res. 17:192-200(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Agy99.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000325 Genomic DNA. Translation: ABL04422.1.
RefSeqYP_905893.1. NC_008611.1.

3D structure databases

ProteinModelPortalA0PQ03.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING362242.MUL_1976.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABL04422; ABL04422; MUL_1976.
GeneID4551195.
KEGGmul:MUL_1976.
PATRIC18170997. VBIMycUlc37413_2347.

Organism-specific databases

GenoListMUL_1976.
CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.
ProtClustDBPRK01966.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_MYCUA
AccessionPrimary (citable) accession number: A0PQ03
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: February 19, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways