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Protein

Triosephosphate isomerase

Gene

tpiA

Organism
Mycobacterium ulcerans (strain Agy99)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).UniRule annotation

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Triosephosphate isomerase (tpiA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei100ElectrophileUniRule annotation1
Active sitei172Proton acceptorUniRule annotation1
Binding sitei178Substrate; via amide nitrogenUniRule annotation1
Binding sitei218SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processGluconeogenesis, Glycolysis, Pentose shunt

Enzyme and pathway databases

UniPathwayiUPA00109; UER00189.
UPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Triosephosphate isomeraseUniRule annotation (EC:5.3.1.1UniRule annotation)
Short name:
TIMUniRule annotation
Short name:
TPIUniRule annotation
Alternative name(s):
Triose-phosphate isomeraseUniRule annotation
Gene namesi
Name:tpiAUniRule annotation
Ordered Locus Names:MUL_1830
OrganismiMycobacterium ulcerans (strain Agy99)
Taxonomic identifieri362242 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000765 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003075091 – 261Triosephosphate isomeraseAdd BLAST261

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

SMRiA0PPN4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni10 – 12Substrate bindingUniRule annotation3
Regioni239 – 240Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the triosephosphate isomerase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000226412.
KOiK01803.
OMAiLCVGEGL.
OrthoDBiPOG091H02GL.

Family and domain databases

CDDicd00311. TIM. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_B. TIM_B. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR035990. TIM_sf.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiView protein in Pfam
PF00121. TIM. 1 hit.
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiView protein in PROSITE
PS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.

Sequencei

Sequence statusi: Complete.

A0PPN4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRKPLIAGN WKMNLNHFEA IALVQKIAFS LPDKYYDKVD VTVIPPFTDL
60 70 80 90 100
RSVQTLVDGD KLRLTYGGQD LSQHDSGAYT GDISGAFLAK LGCSFVVVGH
110 120 130 140 150
SERRTYHNED DALVAAKAAA ALKHDLTPIV CIGEHLDVRE AGNHVAHNVE
160 170 180 190 200
QLRGSLSGLS AEQISKVVIA YEPVWAIGTG RVAGAADAQE VCAAIRSELG
210 220 230 240 250
SLASPQIADA VRVLYGGSVN AKNIGELIAQ ADVDGGLVGG ASLDGEQFAT
260
LAAIAAGGPL P
Length:261
Mass (Da):27,325
Last modified:January 9, 2007 - v1
Checksum:i30BC67E4640E6D15
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000325 Genomic DNA. Translation: ABL04303.1.
RefSeqiWP_011739923.1. NC_008611.1.

Genome annotation databases

EnsemblBacteriaiABL04303; ABL04303; MUL_1830.
GeneIDi34343749.
KEGGimul:MUL_1830.

Similar proteinsi

Entry informationi

Entry nameiTPIS_MYCUA
AccessioniPrimary (citable) accession number: A0PPN4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: January 9, 2007
Last modified: November 22, 2017
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families