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A0PP05

- BIOB_MYCUA

UniProt

A0PP05 - BIOB_MYCUA

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Protein
Biotin synthase
Gene
bioB, MUL_1563
Organism
Mycobacterium ulcerans (strain Agy99)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi86 – 861Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi90 – 901Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi93 – 931Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi129 – 1291Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi162 – 1621Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi221 – 2211Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi291 – 2911Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:MUL_1563
OrganismiMycobacterium ulcerans (strain Agy99)
Taxonomic identifieri362242 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000765: Chromosome

Organism-specific databases

GenoListiMUL_1563.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 350350Biotin synthaseUniRule annotation
PRO_0000381488Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi362242.MUL_1563.

Structurei

3D structure databases

ProteinModelPortaliA0PP05.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiDETQALC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A0PP05-1 [UniParc]FASTAAdd to Basket

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MVTQAATRPS NDAGQDGVTE PDILAVARQQ VLERGEGLNQ EQVLQVLQLS    50
EDRLEELLVL AHDVRMRWCG PEVEVEGIIS LKTGGCPEDC HFCSQSGLFS 100
SPVRSAWLDI PSLVEAAKQT AKSGATEFCI VAAVRGPDAR LLSQVAAGIE 150
AIRNEVEINV ACSLGMLTAE QVEQLAAMGV HRYNHNLETA RSYFTNVVTT 200
HTWEERWQTL TMVRDAGMEV CCGGILGMGE TLEQRAEFAA NLAELDPDEV 250
PLNFLNPRPG TPFGDLEVLP AGEALKAVGA FRLALPRTML RFAGGREITL 300
GDLGAKRGIL GGINAVIVGN YLTTLGRPAE ADLELLEDLQ MPLKALNASL 350
Length:350
Mass (Da):37,726
Last modified:January 9, 2007 - v1
Checksum:i27E01439AE2971D5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000325 Genomic DNA. Translation: ABL04074.1.
RefSeqiYP_905545.1. NC_008611.1.

Genome annotation databases

EnsemblBacteriaiABL04074; ABL04074; MUL_1563.
GeneIDi4552336.
KEGGimul:MUL_1563.
PATRICi18170039. VBIMycUlc37413_1874.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000325 Genomic DNA. Translation: ABL04074.1 .
RefSeqi YP_905545.1. NC_008611.1.

3D structure databases

ProteinModelPortali A0PP05.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 362242.MUL_1563.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABL04074 ; ABL04074 ; MUL_1563 .
GeneIDi 4552336.
KEGGi mul:MUL_1563.
PATRICi 18170039. VBIMycUlc37413_1874.

Organism-specific databases

GenoListi MUL_1563.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi DETQALC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer."
    Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M.
    , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T.
    Genome Res. 17:192-200(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Agy99.

Entry informationi

Entry nameiBIOB_MYCUA
AccessioniPrimary (citable) accession number: A0PP05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 9, 2007
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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