A0PNZ5 (A0PNZ5_MYCUA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase PIRNR PIRNR006337
Short name=MTHase PIRNR PIRNR006337
EC=3.2.1.141 PIRNR PIRNR006337

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase PIRNR PIRNR006337
Maltooligosyl trehalose trehalohydrolase PIRNR PIRNR006337

Gene names

Name:	treZ EMBL ABL04064.1
Ordered Locus Names:	MUL_1553

Organism

Mycobacterium ulcerans (strain Agy99) [Complete proteome] [HAMAP]

Taxonomic identifier

362242 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	587 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan. PIRNR PIRNR006337
Pathway	Glycan biosynthesis; trehalose biosynthesis. PIRNR PIRNR006337
Subcellular location	Cytoplasm By similarity PIRSR PIRSR006337-1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. PIRNR PIRNR006337

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR006337 Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	244	1	Nucleophile By similarity PIRSR PIRSR006337-1
	Active site	281	1	Proton donor By similarity PIRSR PIRSR006337-1
	Site	381	1	Transition state stabilizer By similarity PIRSR PIRSR006337-3

Sequences

	Sequence		Length	Mass (Da)
	A0PNZ5 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 062DD531EB70AE6F	FASTA	587	64,464
	10 20 30 40 50 60 MTEFRVWAPK PELVRLDVEG GVYPMRRTDD GWWHTAIETA PAARYGFLLD DDPKVLPDPR 70 80 90 100 110 120 SARQPDGVHA RSQLWNPAAA TWTDGAWAGR SVTGAVIYEL HVGTFTAAGT FDSAIEKLDY 130 140 150 160 170 180 LVDLGIDFVE LMPVNSFAGT HGWGYDGVLW YSVHEPYGGP AGLVRFVDAC HARGLGVLID 190 200 210 220 230 240 AVFNHLGPSG NYLPRFGPYL SSASNPWGEG INIADADSDE VRRYIIDVAL RWMREFHADG 250 260 270 280 290 300 LRLDAVHALV DTTAVPILEE LATETDWLSK QVGRPLSLVA ESDLNDPRLI TPRDGGGYGI 310 320 330 340 350 360 TAQWDDDIHH AIHTAVSGER QGYYADFGAN SDSMATLAHT LRRGFFHAGT YSSFRRRRHG 370 380 390 400 410 420 RPLDTATIPA TRLLAYTCTH DQVGNRALGD RPSQNLTSGQ LAIKAALVLG SPYTAMLFMG 430 440 450 460 470 480 EEWGASSPFQ FFSSHPEPEL AVATAQGRKA EFAEHGWDAD EIPDPQDPQT FLRSKLVWGE 490 500 510 520 530 540 GDSGEHAQLL RFYRDLIALR HHETDLADPW LDHLVIDYDE QQSWIIMRRG RFGIACNLGT 550 560 570 580 EATTVPLSGA LVLGWGEPNT SDSGTELAGH SVAVLRCDQS TAPAEPA

References

[1]

"Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer." Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M. , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T. Genome Res. 17:192-200(2007) [PubMed: 17210928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000325 Genomic DNA. Translation: ABL04064.1.
RefSeq	YP_905535.1. NC_008611.1.
3D structure databases
ProteinModelPortal	A0PNZ5.
ModBase	Search...
Protein-protein interaction databases
STRING	A0PNZ5.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000075381; EBMYCP00000073386; EBMYCG00000075376.
GeneID	4552326.
GenomeReviews	Gene locus MUL_1553 in contig CP000325_GR.
KEGG	mul:MUL_1553.
PATRIC	18170019. VBIMycUlc37413_1864.
Organism-specific databases
GenoList	MUL_1553.
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
GeneTree	EBGT00050000016657.
HOGENOM	HBG367595.
OMA	EGFVYQG.
ProtClustDB	CLSK799816.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K01236.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A0PNZ5_MYCUA

Accession

Primary (citable) accession number: A0PNZ5

Entry history

Integrated into UniProtKB/TrEMBL:	January 9, 2007
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)