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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Mycobacterium ulcerans (strain Agy99)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei81SubstrateUniRule annotation1
Metal bindingi229ManganeseUniRule annotation1
Metal bindingi249Manganese; via tele nitrogenUniRule annotation1
Binding sitei271SubstrateUniRule annotation1
Active sitei273UniRule annotation1
Metal bindingi296ManganeseUniRule annotation1
Binding sitei389GTPUniRule annotation1
Binding sitei420GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi272 – 277GTPUniRule annotation6
Nucleotide bindingi515 – 518GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:MUL_1101
OrganismiMycobacterium ulcerans (strain Agy99)
Taxonomic identifieri362242 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000765 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000602971 – 609Phosphoenolpyruvate carboxykinase [GTP]Add BLAST609

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA0PMX1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni220 – 222Substrate bindingUniRule annotation3
Regioni387 – 389Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000191700.
KOiK01596.
OMAiYGENMRV.
OrthoDBiPOG091H0B4F.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0PMX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSATIPGLD TAPTNHQGLL SWVQEVAELT QPDRVVFADG SDEEFHRLSA
60 70 80 90 100
QLVDAGTFTR LNDEKFPNSY LALSDPSDVA RVESRTFICS EREIDAGPTN
110 120 130 140 150
NWMNPSEMRT LMTDLYRGCM RGRTMYVVPF CMGPLGAEDP KLGVEITDSE
160 170 180 190 200
YVVVSMKVMT RMGTAALEKM GQDGFFVKAL HSVGAPLEDG QADVPWPCSD
210 220 230 240 250
TKYITHFPET REIWSYGSGY GGNALLGKKC YSLRIASAMA RDEGWLAEHM
260 270 280 290 300
LILKLISPEN KAYYIAAAFP SACGKTNLAM LQPTIPGWRA ETLGDDIAWM
310 320 330 340 350
RFGKDGRLYA VNPEFGFFGV APGTNWKSNP NAMRTIAAGN TVFTNVALTD
360 370 380 390 400
DGEVWWEGLE GDPQHLVDWK GNEWYFRETE TTAAHPNSRY CTPMSQCPIL
410 420 430 440 450
APEWDDPQGV PISAILFGGR RKTTVPLVTQ ARDWQHGVFI GATLGSEQTA
460 470 480 490 500
AAEGKVGNVR RDPMAMLPFM GYNVGDYVQH WIDIGKNSDE SKLPQVFFVN
510 520 530 540 550
WFRRGEDHRF LWPGFGENSR VMKWIVDRIE HKAGGKTTPI GTVPTVEDLD
560 570 580 590 600
LEGLDANPAD VSEALAVNAQ EWREELPLIE EWLQFIGEKL PTGIKDEFDA

LKERLRDAE
Length:609
Mass (Da):67,846
Last modified:January 9, 2007 - v1
Checksum:i9D4A019065B6CF75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000325 Genomic DNA. Translation: ABL03690.1.

Genome annotation databases

EnsemblBacteriaiABL03690; ABL03690; MUL_1101.
KEGGimul:MUL_1101.
PATRICi18168981. VBIMycUlc37413_1351.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000325 Genomic DNA. Translation: ABL03690.1.

3D structure databases

ProteinModelPortaliA0PMX1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL03690; ABL03690; MUL_1101.
KEGGimul:MUL_1101.
PATRICi18168981. VBIMycUlc37413_1351.

Phylogenomic databases

HOGENOMiHOG000191700.
KOiK01596.
OMAiYGENMRV.
OrthoDBiPOG091H0B4F.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKG_MYCUA
AccessioniPrimary (citable) accession number: A0PMX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: January 9, 2007
Last modified: October 5, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.