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A0PMX1

- PCKG_MYCUA

UniProt

A0PMX1 - PCKG_MYCUA

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Mycobacterium ulcerans (strain Agy99)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (09 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei81 – 811SubstrateUniRule annotation
    Binding sitei222 – 2221Substrate; via amide nitrogenUniRule annotation
    Metal bindingi229 – 2291ManganeseUniRule annotation
    Binding sitei229 – 2291SubstrateUniRule annotation
    Metal bindingi249 – 2491Manganese; via tele nitrogenUniRule annotation
    Binding sitei271 – 2711SubstrateUniRule annotation
    Active sitei273 – 2731UniRule annotation
    Metal bindingi296 – 2961ManganeseUniRule annotation
    Binding sitei389 – 3891GTPUniRule annotation
    Binding sitei420 – 4201GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi272 – 2776GTPUniRule annotation
    Nucleotide bindingi515 – 5184GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Ordered Locus Names:MUL_1101
    OrganismiMycobacterium ulcerans (strain Agy99)
    Taxonomic identifieri362242 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000765: Chromosome

    Organism-specific databases

    GenoListiMUL_1101.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 609609Phosphoenolpyruvate carboxykinase [GTP]PRO_1000060297Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi362242.MUL_1101.

    Structurei

    3D structure databases

    ProteinModelPortaliA0PMX1.
    SMRiA0PMX1. Positions 12-605.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni387 – 3893Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiWDNPAGV.
    OrthoDBiEOG6MPWQS.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0PMX1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSATIPGLD TAPTNHQGLL SWVQEVAELT QPDRVVFADG SDEEFHRLSA    50
    QLVDAGTFTR LNDEKFPNSY LALSDPSDVA RVESRTFICS EREIDAGPTN 100
    NWMNPSEMRT LMTDLYRGCM RGRTMYVVPF CMGPLGAEDP KLGVEITDSE 150
    YVVVSMKVMT RMGTAALEKM GQDGFFVKAL HSVGAPLEDG QADVPWPCSD 200
    TKYITHFPET REIWSYGSGY GGNALLGKKC YSLRIASAMA RDEGWLAEHM 250
    LILKLISPEN KAYYIAAAFP SACGKTNLAM LQPTIPGWRA ETLGDDIAWM 300
    RFGKDGRLYA VNPEFGFFGV APGTNWKSNP NAMRTIAAGN TVFTNVALTD 350
    DGEVWWEGLE GDPQHLVDWK GNEWYFRETE TTAAHPNSRY CTPMSQCPIL 400
    APEWDDPQGV PISAILFGGR RKTTVPLVTQ ARDWQHGVFI GATLGSEQTA 450
    AAEGKVGNVR RDPMAMLPFM GYNVGDYVQH WIDIGKNSDE SKLPQVFFVN 500
    WFRRGEDHRF LWPGFGENSR VMKWIVDRIE HKAGGKTTPI GTVPTVEDLD 550
    LEGLDANPAD VSEALAVNAQ EWREELPLIE EWLQFIGEKL PTGIKDEFDA 600
    LKERLRDAE 609
    Length:609
    Mass (Da):67,846
    Last modified:January 9, 2007 - v1
    Checksum:i9D4A019065B6CF75
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000325 Genomic DNA. Translation: ABL03690.1.
    RefSeqiYP_905161.1. NC_008611.1.

    Genome annotation databases

    EnsemblBacteriaiABL03690; ABL03690; MUL_1101.
    GeneIDi4553521.
    KEGGimul:MUL_1101.
    PATRICi18168981. VBIMycUlc37413_1351.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000325 Genomic DNA. Translation: ABL03690.1 .
    RefSeqi YP_905161.1. NC_008611.1.

    3D structure databases

    ProteinModelPortali A0PMX1.
    SMRi A0PMX1. Positions 12-605.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 362242.MUL_1101.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABL03690 ; ABL03690 ; MUL_1101 .
    GeneIDi 4553521.
    KEGGi mul:MUL_1101.
    PATRICi 18168981. VBIMycUlc37413_1351.

    Organism-specific databases

    GenoListi MUL_1101.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi WDNPAGV.
    OrthoDBi EOG6MPWQS.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer."
      Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M.
      , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T.
      Genome Res. 17:192-200(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Agy99.

    Entry informationi

    Entry nameiPCKG_MYCUA
    AccessioniPrimary (citable) accession number: A0PMX1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3