ID   MHPB_MYCUA              Reviewed;         313 AA.
AC   A0PL52;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 2.
DT   16-JUN-2009, entry version 18.
DE   RecName: Full=2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase;
DE            EC=1.13.11.16;
GN   Name=mhpB; OrderedLocusNames=MUL_0347;
OS   Mycobacterium ulcerans (strain Agy99).
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium.
OX   NCBI_TaxID=362242;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17210928; DOI=10.1101/gr.5942807;
RA   Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T.,
RA   Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L.,
RA   Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C.,
RA   Jones L.M., Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T.;
RT   "Reductive evolution and niche adaptation inferred from the genome of
RT   Mycobacterium ulcerans, the causative agent of Buruli ulcer.";
RL   Genome Res. 17:192-200(2007).
CC   -!- FUNCTION: Catalyzes the non-heme iron(II)-dependent oxidative
CC       cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-
CC       dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-
CC       hydroxy-6-ketononatrienedioate, respectively (By similarity).
CC   -!- CATALYTIC ACTIVITY: 3-(2,3-dihydroxyphenyl)propanoate + O(2) = 2-
CC       hydroxy-6-oxonona-2,4-diene-1,9-dioate.
CC   -!- CATALYTIC ACTIVITY: 2,3-dihydroxicinnamic acid + O(2) = 2-hydroxy-
CC       6-oxonona-2,4,7-triene-1,9-dioate.
CC   -!- COFACTOR: Fe(2+) ion (By similarity).
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropionic acid
CC       degradation.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the ligB/mhpB extradiol dioxygenase family.
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DR   EMBL; CP000325; ABL03071.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; YP_904542.1; -.
DR   GeneID; 4550878; -.
DR   GenomeReviews; CP000325_GR; MUL_0347.
DR   KEGG; mul:MUL_0347; -.
DR   BuruList; MUL_0347; -.
DR   GO; GO:0008669; F:2,3-dihydroxy-phenylpropionate 1,2-dioxygen...; IEA:HAMAP.
DR   GO; GO:0047070; F:3-carboxyethylcatechol 2,3-dioxygenase acti...; IEA:EC.
DR   GO; GO:0008198; F:ferrous iron binding; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01653; -; 1.
DR   InterPro; IPR004183; Xdiol_dOase_3B.
DR   Pfam; PF02900; LigB; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Complete proteome; Dioxygenase;
KW   Iron; Oxidoreductase.
FT   CHAIN         1    313       2,3-dihydroxyphenylpropionate/2,3-
FT                                dihydroxicinnamic acid 1,2-dioxygenase.
FT                                /FTId=PRO_0000337659.
FT   ACT_SITE    115    115       Proton donor (By similarity).
FT   ACT_SITE    179    179       Proton acceptor (By similarity).
SQ   SEQUENCE   313 AA;  33564 MW;  0EB633671CBFC800 CRC64;
     MALALCRMSH SPLLNLPGPR LDLLDEVHAA IAEAAEFVRA YDPDLVVIFS PDHYNGFFYR
     AMHPFCIGMY ASAVGDYGTH IGALDVPTDL AADCAKAVLG ADVDVAVSAS MDVDHGTVQP
     LEKLFGTATA RPVIPIFINA IAAPLGPLRR CRALGTAVGT FLSTLDLRVL VIGSGGLSHS
     PPVPTLHSAD PQVRERIVHG QPLTPAQRQA RQTVVMEAAK SFAAGNSDLQ PLNPAWDQRF
     LEIIDNGHLS DLDRWSNSFV THEGGSLAHE IRTWISAFAA MAVAGPYQTK VRYYKQAADL
     IAGFAIRTAV PIP
//