Reviewed, UniProtKB/Swiss-Prot A0PL16 (KDC_MYCUA)

Names and origin

Protein names

Recommended name:
    Alpha-keto-acid decarboxylase
      Short name=KDC
    EC=4.1.1.-

Gene names

Name:	kdc
Ordered Locus Names:	MUL_0302

Organism

Mycobacterium ulcerans (strain Agy99) [Complete proteome] [HAMAP]

Taxonomic identifier

362242 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Decarboxylates branched-chain and aromatic alpha-keto acids to aldehydes.
Cofactor	Binds 1 metal ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding Thiamine pyrophosphate
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	carboxy-lyase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW thiamin pyrophosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 566	566	Alpha-keto-acid decarboxylase		PRO_0000333753
Regions
	Region	396 – 478	83	Thiamine pyrophosphate binding By similarity
Sites
	Metal binding	446	1	Magnesium By similarity
	Metal binding	473	1	Magnesium By similarity
	Metal binding	475	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	61	1	Thiamine pyrophosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0PL16-1 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 34EE33CEE64CFDEE	FASTA	566	60,326
	10 20 30 40 50 60 MTLLENDAAT DPVYTVGDYL LDRLAELGVS EIFGVPGDYN LEFLDHIVAH PIIRWVGSAN 70 80 90 100 110 120 ELNAGYAADG YGRLRGMSAV VTTFGVGELS ATNAIAGSYA EHVPVVHIVG GPSKDAQGAR 130 140 150 160 170 180 RALHHSLGDG DFEHFFRISR EITCAQANLM PATACREIDR VICEVREQKR PGYLLLSTDV 190 200 210 220 230 240 ARFPTEPPGA PLPPLAGGTS PRALSLFTRA AADLIGDHQL TVLADLLVHR LQAIKELEAL 250 260 270 280 290 300 LSADVVPHAT LMWGKSLLDE SSANFLGIYA GAASAEPVRK AIEQAPVLVT AGVVFTDMVS 310 320 330 340 350 360 GFFSQRIDPA RTIDIGQYQS SVADQVFAPL EMGAALQAVA TILTKRGISS PPVAVPPAEP 370 380 390 400 410 420 GPPTPRRDEP LNQEMLWNRL CEALTPGNVV LADQGTSFYG MADHRLPQGV TFIGQPLWGS 430 440 450 460 470 480 IGYTLPAALG AAVAHPDRRT VLLIGDGAAQ LTVQELGIFS REGLSPVIVV VNNDGYTVER 490 500 510 520 530 540 AIHGETATYN DIVSWRWTDV PGALGVTNHL AMRAENYGEL DDALTAAAEQ QDRMVVVEAV 550 560 LPRLDVPPLL DELVGSLSPP ECGGRS

References

[1]

"Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer." Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M. , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T. Genome Res. 17:192-200(2007) [PubMed: 17210928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000325 Genomic DNA. Translation: ABL03035.1.
RefSeq	YP_904506.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4549594.
GenomeReviews	Gene locus MUL_0302 in contig CP000325_GR.
KEGG	mul:MUL_0302.
Organism-specific databases
BuruList	MUL_0302.
CMR	Search...
Phylogenomic databases
OMA	A0PL16. CITERRP.
Family and domain databases
InterPro	IPR012110. Pyruvt_ip_decrb. IPR000399. TPP_bd_CS. IPR012001. TPP_bd_enzyme_N. IPR011766. TPP_enzyme_bd_C. IPR012000. TPP_enzyme_M. [Graphical view]
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
PIRSF	PIRSF036565. Pyruvt_ip_decrb. 1 hit.
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KDC_MYCUA

Accession

Primary (citable) accession number: A0PL16

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	January 9, 2007
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)