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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Mycobacterium ulcerans (strain Agy99)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei748 – 7481ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:MUL_0056
OrganismiMycobacterium ulcerans (strain Agy99)
Taxonomic identifieri362242 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
ProteomesiUP000000765 Componenti: Chromosome

Organism-specific databases

GenoListiMUL_0056.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 976976Leucine--tRNA ligasePRO_1000009376Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliA0PKG2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi86 – 9712"HIGH" regionAdd
BLAST
Motifi745 – 7495"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0PKG2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTESPTTTPG STSGAPSGVP SGVNDAESDA PRHRYTAELA AGVERTWQQN
60 70 80 90 100
WARLGTFNVP NPVGSLAPSD GSPVPEDKLF VQDMFPYPSG EGLHVGHPLG
110 120 130 140 150
YIATDVFARY HRMKGRNVLH ALGFDAFGLP AEQYAVQTGT HPRTRTEANV
160 170 180 190 200
VNFRRQLGRL GLGHDSRRSF STTDVEFYKW TQWIFLQIYN AWFDAAANKA
210 220 230 240 250
RPISELVAEF DSGARSLVDG RDWSTLSAGE RADVIDDHRL VYRADSMVNW
260 270 280 290 300
CPGLGTVLAN EEVTSDGRSD RGNFPVFRKR LRQWMMRITA YSDRLLDDLD
310 320 330 340 350
VLDWPDQVKT MQRNWIGRST GASALFTATR SNGETVGLEV FTTRPDTLFG
360 370 380 390 400
ATYLVLAPEH DLVDDLVGAG WPAGVDPLWT GGGATPAEAV AAYRRAIAVK
410 420 430 440 450
SDLERQESKE KTGVFLGSHA INPATGQPVP IFIADYVLAG YGTGAIMAVP
460 470 480 490 500
GHDQRDWDFA RALGLPVVEV IAGGDISQAA YTGDGVLVNS GFLDGMSVGE
510 520 530 540 550
AKQAITARLE SDGYGQARIE FKLRDWLFAR QRYWGEPFPI VYDADGRPHA
560 570 580 590 600
LDESALPVEL PDVPDYSPVL FDPDDANSEP SPPLGKATEW LHVELDLGDG
610 620 630 640 650
LKPYSRDTNV MPQWAGSSWY ELRYTDPHNA DRFCAKENET YWMGPRPAEH
660 670 680 690 700
GPDDPGGVDL YVGGAEHAVL HLLYARFWHK VLYDLGHVSS REPYRKLINQ
710 720 730 740 750
GYIQAFAYTD ARGSYVPAEE VIERDGGFVY PGADGEIEVF QEFGKIGKSL
760 770 780 790 800
KNSISPDEIC DDYGADTLRV YEMSMGPIEA SRPWATKDVI GAHRFLQRVW
810 820 830 840 850
RLVIDENTGE ILVADTPAEL DTDTLRALHR AIAGVAEDYA ALRNNTAVAK
860 870 880 890 900
LIEYTNFLTK RHRDAVPRAV IEPLVLMVAP LAPHLAEELW QRLGHTTSLA
910 920 930 940 950
HGPFPAADPA YLIDDTVEYP VQVNGKVRGR VVVAADADDD AVKAAALADQ
960 970
KVQAFLAGAS PRKVIVVAGR LVNLVV
Length:976
Mass (Da):107,185
Last modified:January 9, 2007 - v1
Checksum:i42E0040F495963EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000325 Genomic DNA. Translation: ABL02831.1.
RefSeqiWP_011738456.1. NC_008611.1.
YP_904302.1. NC_008611.1.

Genome annotation databases

EnsemblBacteriaiABL02831; ABL02831; MUL_0056.
GeneIDi4550140.
KEGGimul:MUL_0056.
PATRICi18166538. VBIMycUlc37413_0148.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000325 Genomic DNA. Translation: ABL02831.1.
RefSeqiWP_011738456.1. NC_008611.1.
YP_904302.1. NC_008611.1.

3D structure databases

ProteinModelPortaliA0PKG2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL02831; ABL02831; MUL_0056.
GeneIDi4550140.
KEGGimul:MUL_0056.
PATRICi18166538. VBIMycUlc37413_0148.

Organism-specific databases

GenoListiMUL_0056.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer."
    Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M.
    , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T.
    Genome Res. 17:192-200(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Agy99.

Entry informationi

Entry nameiSYL_MYCUA
AccessioniPrimary (citable) accession number: A0PKG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: July 22, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.