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A0PKG2 (SYL_MYCUA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:MUL_0056
OrganismMycobacterium ulcerans (strain Agy99) [Complete proteome] [HAMAP]
Taxonomic identifier362242 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length976 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 976976Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009376

Regions

Motif86 – 9712"HIGH" region HAMAP-Rule MF_00049
Motif745 – 7495"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7481ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A0PKG2 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: 42E0040F495963EA

FASTA976107,185
        10         20         30         40         50         60 
MTESPTTTPG STSGAPSGVP SGVNDAESDA PRHRYTAELA AGVERTWQQN WARLGTFNVP 

        70         80         90        100        110        120 
NPVGSLAPSD GSPVPEDKLF VQDMFPYPSG EGLHVGHPLG YIATDVFARY HRMKGRNVLH 

       130        140        150        160        170        180 
ALGFDAFGLP AEQYAVQTGT HPRTRTEANV VNFRRQLGRL GLGHDSRRSF STTDVEFYKW 

       190        200        210        220        230        240 
TQWIFLQIYN AWFDAAANKA RPISELVAEF DSGARSLVDG RDWSTLSAGE RADVIDDHRL 

       250        260        270        280        290        300 
VYRADSMVNW CPGLGTVLAN EEVTSDGRSD RGNFPVFRKR LRQWMMRITA YSDRLLDDLD 

       310        320        330        340        350        360 
VLDWPDQVKT MQRNWIGRST GASALFTATR SNGETVGLEV FTTRPDTLFG ATYLVLAPEH 

       370        380        390        400        410        420 
DLVDDLVGAG WPAGVDPLWT GGGATPAEAV AAYRRAIAVK SDLERQESKE KTGVFLGSHA 

       430        440        450        460        470        480 
INPATGQPVP IFIADYVLAG YGTGAIMAVP GHDQRDWDFA RALGLPVVEV IAGGDISQAA 

       490        500        510        520        530        540 
YTGDGVLVNS GFLDGMSVGE AKQAITARLE SDGYGQARIE FKLRDWLFAR QRYWGEPFPI 

       550        560        570        580        590        600 
VYDADGRPHA LDESALPVEL PDVPDYSPVL FDPDDANSEP SPPLGKATEW LHVELDLGDG 

       610        620        630        640        650        660 
LKPYSRDTNV MPQWAGSSWY ELRYTDPHNA DRFCAKENET YWMGPRPAEH GPDDPGGVDL 

       670        680        690        700        710        720 
YVGGAEHAVL HLLYARFWHK VLYDLGHVSS REPYRKLINQ GYIQAFAYTD ARGSYVPAEE 

       730        740        750        760        770        780 
VIERDGGFVY PGADGEIEVF QEFGKIGKSL KNSISPDEIC DDYGADTLRV YEMSMGPIEA 

       790        800        810        820        830        840 
SRPWATKDVI GAHRFLQRVW RLVIDENTGE ILVADTPAEL DTDTLRALHR AIAGVAEDYA 

       850        860        870        880        890        900 
ALRNNTAVAK LIEYTNFLTK RHRDAVPRAV IEPLVLMVAP LAPHLAEELW QRLGHTTSLA 

       910        920        930        940        950        960 
HGPFPAADPA YLIDDTVEYP VQVNGKVRGR VVVAADADDD AVKAAALADQ KVQAFLAGAS 

       970 
PRKVIVVAGR LVNLVV 

« Hide

References

[1]"Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer."
Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M. expand/collapse author list , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T.
Genome Res. 17:192-200(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Agy99.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000325 Genomic DNA. Translation: ABL02831.1.
RefSeqYP_904302.1. NC_008611.1.

3D structure databases

ProteinModelPortalA0PKG2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING362242.MUL_0056.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABL02831; ABL02831; MUL_0056.
GeneID4550140.
KEGGmul:MUL_0056.
PATRIC18166538. VBIMycUlc37413_0148.

Organism-specific databases

GenoListMUL_0056.
CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_MYCUA
AccessionPrimary (citable) accession number: A0PKG2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: May 14, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries