A0PKB2 (DNAA_MYCUA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromosomal replication initiator protein DnaA

Gene names

Name:	dnaA
Ordered Locus Names:	MUL_0001

Organism

Mycobacterium ulcerans (strain Agy99) [Complete proteome] [HAMAP]

Taxonomic identifier

362242 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids By similarity. HAMAP MF_00377
Subcellular location	Cytoplasm By similarity HAMAP MF_00377.
Sequence similarities	Belongs to the DnaA family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA-dependent DNA replication initiation Inferred from electronic annotation. Source: InterPro regulation of DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA replication origin binding Inferred from electronic annotation. Source: InterPro nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Chromosomal replication initiator protein DnaA HAMAP MF_00377		PRO_1000048675
Regions
	Nucleotide binding	211 – 218	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A0PKB2 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 9A3B34DC555860C0	FASTA	510	56,777
	10 20 30 40 50 60 MTNDPGSGFA AVWNAVVAEL NGEPNTDGDA GTGTTLTSPL TPQQRAWLNL VQPLTIVEGF 70 80 90 100 110 120 ALLSVPSSFV QNEIERHLRT PITAALSRRL GQQIQLGVRI APPPADDDDD SVAAAVEDPG 130 140 150 160 170 180 LEASPETSQE VSDEIDDFGE NAPKSRQSWP THFKKRSTDA DTSASADGTS LNRRYTFDTF 190 200 210 220 230 240 VIGASNRFAH AATLAIAEAP ARAYNPLFIW GESGLGKTHL LHAAGNYAQR LFPGMRVKYV 250 260 270 280 290 300 STEEFTNDFI NSLRDDRKVA FKRSYRDVDV LLVDDIQFIE GKEGIQEEFF HTFNTLHNAN 310 320 330 340 350 360 KQIVISSDRP PKQLATLEDR LRTRFEWGLI TDVQPPELET RIAILRKKAQ MERLAVPDDV 370 380 390 400 410 420 LELIASSIER NIRELEGALI RVTAFASLNK TPIDKALAEI VLRDLIADAD TMQISAATIM 430 440 450 460 470 480 AATVEYFDTT VEELRGPGKT RALAQSRQIA MYLCRELTDL SLPKIGQAFG RDHTTVMYAQ 490 500 510 RKILSEMAER REVFDHVKEL TTRIRQRSKR

References

[1]

"Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer." Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T., Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J., Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M. , Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T. Genome Res. 17:192-200(2007) [PubMed: 17210928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Agy99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000325 Genomic DNA. Translation: ABL02781.1.
RefSeq	YP_904252.1. NC_008611.1.
3D structure databases
ProteinModelPortal	A0PKB2.
ModBase	Search...
Protein-protein interaction databases
STRING	A0PKB2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000075451; EBMYCP00000073456; EBMYCG00000075446.
GeneID	4553245.
GenomeReviews	Gene locus MUL_0001 in contig CP000325_GR.
KEGG	mul:MUL_0001.
PATRIC	18166424. VBIMycUlc37413_0094.
Organism-specific databases
GenoList	MUL_0001.
CMR	Search...
Phylogenomic databases
eggNOG	COG0593.
GeneTree	EBGT00050000017320.
HOGENOM	HBG695666.
OMA	ASVHESW.
PhylomeDB	A0PKB2.
ProtClustDB	PRK00149.
Family and domain databases
HAMAP	MF_00377. DnaA_bact. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR001957. Chromosome_initiator_DnaA. IPR020591. Chromosome_initiator_DnaA-like. IPR018312. Chromosome_initiator_DnaA_CS. IPR013159. DnaA_C. IPR013317. DnaA_Hda. IPR010921. Trp_repressor/repl_initiator. [Graphical view]
Gene3D	G3DSA:1.10.1750.10. DnaA_C. 1 hit.
KO	K02313.
Pfam	PF00308. Bac_DnaA. 1 hit. PF08299. Bac_DnaA_C. 1 hit. [Graphical view]
PRINTS	PR00051. DNAA.
SMART	SM00382. AAA. 1 hit. SM00760. Bac_DnaA_C. 1 hit. [Graphical view]
SUPFAM	SSF48295. Trp_repress_rep. 1 hit.
TIGRFAMs	TIGR00362. DnaA. 1 hit.
PROSITE	PS01008. DNAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAA_MYCUA

Accession

Primary (citable) accession number: A0PKB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program