Reviewed, UniProtKB/Swiss-Prot A0PJ24 (L_BCNVU)

Names and origin

Protein names

Recommended name:
    RNA-directed RNA polymerase
    EC=2.7.7.48
Alternative name(s):
    Protein L

Gene names

Name:

L

Organism

Bear canyon virus (isolate Mouse/United States/AV A0070039/2000) (BCNV)

Taxonomic identifier

192848 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Arenaviridae › Arenavirus › New world arenaviruses

Virus host

Peromyscus californicus (California mouse) [TaxID: 42520]

Protein attributes

Sequence length	2216 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N By similarity. Interacts with protein Z; this interaction inhibits viral transcription and replication By similarity.
Subcellular location	Virion. Host cytoplasm.
Sequence similarities	Belongs to the arenaviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Host cytoplasm Virion
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2216	2216	RNA-directed RNA polymerase		PRO_0000361633
Regions
	Domain	1167 – 1364	198	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	A0PJ24-1 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 50A106A262B03068	FASTA	2,216	255,780
	10 20 30 40 50 60 MSEYLDELKE LIRKWIPDEE MYIEQKTSFL SQVNLRSVVI EGLKLLSIII EIDSCKKHGC 70 80 90 100 110 120 VHNKNKTVNQ ILRDHRIVGP TLPDVVPDGY RVIGSTIILL EAFVRVSHES FEIKYKSDFE 130 140 150 160 170 180 KLMQLSKDLS RCGLTLIPVV DGRSNYYTEH FPDWTIERMR WLILKITNFL RDNGEEIEEL 190 200 210 220 230 240 EYSRLVYSLS NMENKNLGLE SLKILKEEGL DYKAKLMSVM RDGVNSNMSA SECRVEMAKI 250 260 270 280 290 300 YDQFSFLRKN GLYKDVYCKT SRTEIINWLK DHKLILLSGE TRTAMLDERQ CGYCRNHMFR 310 320 330 340 350 360 ILASLIKNKR HYQSLTNPKK CGSIQSHKKL LSDCNKIKGL KVLNTRRFTL LCLDVIILNS 370 380 390 400 410 420 LLELIDAGEI DNEFLVNNHF KSVNDRLVSI DLIIDRLNKK LMSKPNWIGS VKYKMKRTLE 430 440 450 460 470 480 IHGLYYVSKW LKQVDIDSWY EFKMMREHSD KCVKPTLKYK KDAARKCGQP EFGSSTILDD 490 500 510 520 530 540 EVFLEYLEAL STLSLGLVNS MKTSSAAKFL INDKSNYFGT VQCNECYFQD LDKSYNSLLI 550 560 570 580 590 600 YQKTGERSRC YGLMFKSEQF ENVYEVGESF YADPKRFFLP IMSSEVILKM CVEMLSWLDW 610 620 630 640 650 660 LSEQELKAFK SKLYTLIINI LTVPSKRVQV YLQGFRYLIM AFVNELHFKE LQNKLKVQPL 670 680 690 700 710 720 TISECYVFTL MDDLVHLLLT EAQEENMSKV FRFVLNLSYL CHLVTKETPD RLTDQIKCFE 730 740 750 760 770 780 KFLEPKVDFN SVFVNLDSSP HLSGEVEEKF IKDLNRLFSK DLGVEDLKDP GISKELISLC 790 800 810 820 830 840 ASCFNCGLLP MSKVLKHDPQ SPSFTSTALD ISSNKSVVVP KLDEVGETVT QYDYQSLLSS 850 860 870 880 890 900 TVVDMAQSFK DKLKYKLDRK SIQFAIFKRL TNMVLKRKTD HDVKDDLDDE LSEIVDDDTL 910 920 930 940 950 960 RVINDVEANV SECLSKMGKI SRAATVGGQN NLGRFEKIDT LKRLWDRESM NFILMETSLH 970 980 990 1000 1010 1020 EVKDFDPSIF PIEKYKSMCE LVYDSKMKSE FFTDEVLKFC PLDLLVKNLA TKCYLEEDFF 1030 1040 1050 1060 1070 1080 ECFKYILISA GFDNRVGRYD HRSRSRLGFK DEAILIKENS RISSRESNSE AISRRLDKSF 1090 1100 1110 1120 1130 1140 FTNSSLRNLC FYSEESPTYR STVSSSVGKL KFGLSYKEQV GSNRELYVGD LNTKLTSRLI 1150 1160 1170 1180 1190 1200 EDYFESLTSE CKFSCLNNDA EFERALLDMK CVVRLSGLAV SMDHSKWGPY MSPAIFNILF 1210 1220 1230 1240 1250 1260 SNLNLELNDG VFIDKAPIEN LLNWHLHKIV EVPYNVIDAY LKGYTKRRLG LMDRSSTSIT 1270 1280 1290 1300 1310 1320 EDFIFNWFAK GVVPSHISSV LDMGQGILHN TSDYYGLLTE QFILQCLDFI FDIKSTAYTS 1330 1340 1350 1360 1370 1380 SDDEILLSNS PSLKKVDEDS LDINKCQEVL EFHNYLSSKF NKFVSPKTVA GSFASEFKSR 1390 1400 1410 1420 1430 1440 FFIWSQEVPL LTKFVAAALH NVKAKSPHQL AETVDTILDQ CIANGVSIEV VKAISRRTNK 1450 1460 1470 1480 1490 1500 LITYSGHPKN PFLCVENTDL KDWVDGSRGY RLQRSVESLF NDDDLPLTIR NSCRSLFHRI 1510 1520 1530 1540 1550 1560 RSGDIQEEFL INALQTSPDE CLAKMLRLSD VDESTIDKVL EFRWLNLRAH GDLRLVLRTK 1570 1580 1590 1600 1610 1620 VMSGTRILDR EEVPSLVKSV QSKLSKNFVR GAKKIITDAI NKSAFQSSIC SGFIGFCKSM 1630 1640 1650 1660 1670 1680 GSKCVRDGNG SFQYIKHFLK SIILHSHCEV CKPEMSVFCR AALEELKPFS RPIFWDYFSL 1690 1700 1710 1720 1730 1740 TFSNACELGN WVFSNVTIPK RTPTTVNPNF FWPVKPGSHT ELEDKVNMNH VLYSIKRNFP 1750 1760 1770 1780 1790 1800 DLFDEHIAPF LSDLNSLKIS WIQRIKFLDL CVAMDMSSEC LGIISHIMRR KREELYIVKQ 1810 1820 1830 1840 1850 1860 NELSVAHMRD SSPMEAGYQL NSSEICHNFL CQLVFESMLH PVLLTTGQFK KYFWFGEVEL 1870 1880 1890 1900 1910 1920 LPNEADHDLG QLTQFVMDCK TLNISRCMSL DDLDVGYVHS SILMGDIYVN FSSFLHLLDW 1930 1940 1950 1960 1970 1980 ENRRNYKTFD EIILCSREDT IPMEIDFTIS HSRKSFKFKY ERKTNYHIKS KVLVQKVDIE 1990 2000 2010 2020 2030 2040 EAQNQGFDIL ELEVHEIECF VSGSQGNHIS LDGVGLIPLH PLFSGKEFLD VNKLLIKQDE 2050 2060 2070 2080 2090 2100 NFESTHSVFS KVKLNFSNHT KDLKNKYSYK LQGPEYNMNP LHLYRGQIME NNFVISRLDV 2110 2120 2130 2140 2150 2160 QITSRSVFLA LEALESEDRI PFLISLHIYT RSNNKKENSC FIRMTQSDLC LLIDSYEKEF 2170 2180 2190 2200 2210 TEVLKSLSDW MDFGDFALCF SNNLNCIMIA DPDGQFKLKG RQCRKVSSAS APLEID

References

[1]	"Principal host relationships and evolutionary history of the North American arenaviruses." Cajimat M.N., Milazzo M.L., Hess B.D., Rood M.P., Fulhorst C.F. Virology 367:235-243(2007) [PubMed: 17624390] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY924390 Genomic RNA. Translation: AAX99344.1.
RefSeq	YP_001649225.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5848382.
Family and domain databases
InterPro	IPR014386. RNA-dir_pol_arenavirus. IPR007099. RNA-dir_pol_NSvirus. IPR010453. RNA_pol_arenavir. [Graphical view]
Pfam	PF06317. Arena_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000836. L_ArenaV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_BCNVU

Accession

Primary (citable) accession number: A0PJ24

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	January 9, 2007
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)