ID   ZN148_DANRE             Reviewed;         808 AA.
AC   A0MS83; B0S5L9; B3DIY1;
DT   25-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 2.
DT   27-MAR-2024, entry version 107.
DE   RecName: Full=Zinc finger protein 148;
DE   AltName: Full=Transcription factor ZBP-89;
DE   AltName: Full=Zinc finger DNA-binding protein 89;
GN   Name=znf148; Synonyms=zbp89; ORFNames=si:dkey-101k6.6;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RC   TISSUE=Embryo;
RX   PubMed=16914492; DOI=10.1242/dev.02540;
RA   Li X., Xiong J.-W., Shelley C.S., Park H., Arnaout M.A.;
RT   "The transcription factor ZBP-89 controls generation of the hematopoietic
RT   lineage in zebrafish and mouse embryonic stem cells.";
RL   Development 133:3641-3650(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in transcriptional regulation. Represses the
CC       transcription of a number of genes. Required for primitive and
CC       definitive hematopoiesis during embryonic development.
CC       {ECO:0000269|PubMed:16914492}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: Detected in posterior lateral mesoderm, head
CC       mesenchyme and the intermediate cell mass in embryos 24 hours after
CC       fertilization. {ECO:0000269|PubMed:16914492}.
CC   -!- MISCELLANEOUS: Knock-down of the znf148 mRNA disrupts both primitive
CC       and definitive hematopoiesis, but does not affect primary blood vessel
CC       formation. Most embryos die before four days after fertilization.
CC   -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC       family. {ECO:0000305}.
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DR   EMBL; EF032416; ABK40532.1; -; mRNA.
DR   EMBL; BX255930; CAQ14829.1; -; Genomic_DNA.
DR   EMBL; BC163289; AAI63289.1; -; mRNA.
DR   RefSeq; NP_001073649.1; NM_001080180.1.
DR   AlphaFoldDB; A0MS83; -.
DR   SMR; A0MS83; -.
DR   STRING; 7955.ENSDARP00000071856; -.
DR   PaxDb; 7955-ENSDARP00000071856; -.
DR   GeneID; 560300; -.
DR   KEGG; dre:560300; -.
DR   AGR; ZFIN:ZDB-GENE-030131-8769; -.
DR   CTD; 7707; -.
DR   ZFIN; ZDB-GENE-030131-8769; znf148.
DR   eggNOG; KOG1721; Eukaryota.
DR   HOGENOM; CLU_025987_1_0_1; -.
DR   InParanoid; A0MS83; -.
DR   OMA; FEQNFKS; -.
DR   OrthoDB; 5318724at2759; -.
DR   PhylomeDB; A0MS83; -.
DR   TreeFam; TF331779; -.
DR   PRO; PR:A0MS83; -.
DR   Proteomes; UP000000437; Chromosome 9.
DR   Bgee; ENSDARG00000055106; Expressed in caudal fin and 24 other cell types or tissues.
DR   ExpressionAtlas; A0MS83; baseline.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0030097; P:hemopoiesis; IMP:ZFIN.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   Gene3D; 3.30.160.60; Classic Zinc Finger; 4.
DR   InterPro; IPR036236; Znf_C2H2_sf.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   PANTHER; PTHR24394; ZINC FINGER PROTEIN; 1.
DR   PANTHER; PTHR24394:SF23; ZINC FINGER PROTEIN 281-LIKE ISOFORM X1; 1.
DR   Pfam; PF00096; zf-C2H2; 2.
DR   SMART; SM00355; ZnF_C2H2; 4.
DR   SUPFAM; SSF57667; beta-beta-alpha zinc fingers; 2.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 4.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 4.
PE   2: Evidence at transcript level;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat; Repressor;
KW   Transcription; Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..808
FT                   /note="Zinc finger protein 148"
FT                   /id="PRO_0000354690"
FT   ZN_FING         180..202
FT                   /note="C2H2-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         208..230
FT                   /note="C2H2-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         236..258
FT                   /note="C2H2-type 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         264..287
FT                   /note="C2H2-type 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   REGION          15..86
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          131..162
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          305..338
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          596..617
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          705..736
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        56..74
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        131..146
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        66
FT                   /note="E -> DD (in Ref. 3; AAI63289)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        325
FT                   /note="K -> T (in Ref. 1; ABK40532)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        429
FT                   /note="Q -> E (in Ref. 3; AAI63289)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   808 AA;  90507 MW;  B87251E494294A77 CRC64;
     MNIDDKLEGM LLKYSPVGLQ HPSRGLGPSR VDGRGRRGSL DMTLGERSLA NHPLLAEDDD
     DEDEEEDDDD DLAGEGLTSH DLISQDDLMV HEETVKNDGQ DEAAFSHRIS NKLHYTLSMP
     VNIKQEMKVP DSLILNKKEK KPGRDPSDCH KKKKRKQRSP AKILTINEDG SLGHQNPKSH
     ICEHCNAAFR TNYHLQRHVF IHTGEKPFQC NQCDMRFIQK YLLQRHEKIH TGEKPFRCDE
     CGMKFIQKYH MERHKRTHSG EKPYQCDYCH QFFSRTDRVL KHRRMCHENK DRKVQKTAVK
     DSPLRTPENL GFSFPAKDCT LPKKKRQKTS DKSRASITNP AVDKVVEADM DEKTEDRLAK
     SECLPLYAVA TKVKDEYVVT DYSVELPDSP PGSRHLAGEE SNDEIISPPK LVLKKIASRR
     GFKQQALEQS QSLSPLSSFE ESKVTRYTFE IVDNKGLLDV ETNPDMESVE ALQVGQAKPT
     GSSTNYDDAM QFVKKRRYLQ AATANTSRDY ALNVSSIVSQ SSVTQASVAT VIDETVPATI
     LSETQTLNVE IKSSNEKNVL PDEVLQTLLD HYSNKANGQA EISFSVADTE VTSSISINSS
     DESSPAEALV TSSQAPPTEK ASLLQEYSKF LQQALERTSQ NDTYLNNQSL TFVNDSASLA
     GQPLFSTEKQ FTSPPRFRPT MNSPLRSTLE KPNFNLLVDT QHSFSFSGDE TNPSSVSPTE
     DFLDQVTSPK KTDPQSISQT FQITTFDQNF RSQFPSSRSG ISPQFSIASG QVTLRGHGGA
     DFPEFSLVNE TRTQLTSSPD ATSSQTFG
//