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A0MS83

- ZN148_DANRE

UniProt

A0MS83 - ZN148_DANRE

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Protein

Zinc finger protein 148

Gene

znf148

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Involved in transcriptional regulation. Represses the transcription of a number of genes. Required for primitive and definitive hematopoiesis during embryonic development.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri180 – 20223C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri208 – 23023C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri236 – 25823C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri264 – 28724C2H2-type 4PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. hemopoiesis Source: ZFIN
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 148
Alternative name(s):
Transcription factor ZBP-89
Zinc finger DNA-binding protein 89
Gene namesi
Name:znf148
Synonyms:zbp89
ORF Names:si:dkey-101k6.6
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 9

Organism-specific databases

ZFINiZDB-GENE-030131-8769. znf148.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 808808Zinc finger protein 148PRO_0000354690Add
BLAST

Expressioni

Developmental stagei

Detected in posterior lateral mesoderm, head mesenchyme and the intermediate cell mass in embryos 24 hours after fertilization.1 Publication

Gene expression databases

BgeeiA0MS83.

Structurei

3D structure databases

ProteinModelPortaliA0MS83.
SMRiA0MS83. Positions 233-263.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi58 – 8730Asp/Glu-richAdd
BLAST
Compositional biasi151 – 1566Poly-Lys

Sequence similaritiesi

Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri180 – 20223C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri208 – 23023C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri236 – 25823C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri264 – 28724C2H2-type 4PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00530000063238.
HOGENOMiHOG000293204.
HOVERGENiHBG054247.
InParanoidiA0MS83.
OMAiNDAYLNN.
OrthoDBiEOG76QFGM.
PhylomeDBiA0MS83.
TreeFamiTF331779.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0MS83-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNIDDKLEGM LLKYSPVGLQ HPSRGLGPSR VDGRGRRGSL DMTLGERSLA
60 70 80 90 100
NHPLLAEDDD DEDEEEDDDD DLAGEGLTSH DLISQDDLMV HEETVKNDGQ
110 120 130 140 150
DEAAFSHRIS NKLHYTLSMP VNIKQEMKVP DSLILNKKEK KPGRDPSDCH
160 170 180 190 200
KKKKRKQRSP AKILTINEDG SLGHQNPKSH ICEHCNAAFR TNYHLQRHVF
210 220 230 240 250
IHTGEKPFQC NQCDMRFIQK YLLQRHEKIH TGEKPFRCDE CGMKFIQKYH
260 270 280 290 300
MERHKRTHSG EKPYQCDYCH QFFSRTDRVL KHRRMCHENK DRKVQKTAVK
310 320 330 340 350
DSPLRTPENL GFSFPAKDCT LPKKKRQKTS DKSRASITNP AVDKVVEADM
360 370 380 390 400
DEKTEDRLAK SECLPLYAVA TKVKDEYVVT DYSVELPDSP PGSRHLAGEE
410 420 430 440 450
SNDEIISPPK LVLKKIASRR GFKQQALEQS QSLSPLSSFE ESKVTRYTFE
460 470 480 490 500
IVDNKGLLDV ETNPDMESVE ALQVGQAKPT GSSTNYDDAM QFVKKRRYLQ
510 520 530 540 550
AATANTSRDY ALNVSSIVSQ SSVTQASVAT VIDETVPATI LSETQTLNVE
560 570 580 590 600
IKSSNEKNVL PDEVLQTLLD HYSNKANGQA EISFSVADTE VTSSISINSS
610 620 630 640 650
DESSPAEALV TSSQAPPTEK ASLLQEYSKF LQQALERTSQ NDTYLNNQSL
660 670 680 690 700
TFVNDSASLA GQPLFSTEKQ FTSPPRFRPT MNSPLRSTLE KPNFNLLVDT
710 720 730 740 750
QHSFSFSGDE TNPSSVSPTE DFLDQVTSPK KTDPQSISQT FQITTFDQNF
760 770 780 790 800
RSQFPSSRSG ISPQFSIASG QVTLRGHGGA DFPEFSLVNE TRTQLTSSPD

ATSSQTFG
Length:808
Mass (Da):90,507
Last modified:November 25, 2008 - v2
Checksum:iB87251E494294A77
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 661E → DD in AAI63289. 1 PublicationCurated
Sequence conflicti325 – 3251K → T in ABK40532. (PubMed:16914492)Curated
Sequence conflicti429 – 4291Q → E in AAI63289. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF032416 mRNA. Translation: ABK40532.1.
BX255930 Genomic DNA. Translation: CAQ14829.1.
BC163289 mRNA. Translation: AAI63289.1.
RefSeqiNP_001073649.1. NM_001080180.1.
UniGeneiDr.76709.

Genome annotation databases

EnsembliENSDART00000077389; ENSDARP00000071856; ENSDARG00000055106.
GeneIDi560300.
KEGGidre:560300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF032416 mRNA. Translation: ABK40532.1 .
BX255930 Genomic DNA. Translation: CAQ14829.1 .
BC163289 mRNA. Translation: AAI63289.1 .
RefSeqi NP_001073649.1. NM_001080180.1.
UniGenei Dr.76709.

3D structure databases

ProteinModelPortali A0MS83.
SMRi A0MS83. Positions 233-263.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSDART00000077389 ; ENSDARP00000071856 ; ENSDARG00000055106 .
GeneIDi 560300.
KEGGi dre:560300.

Organism-specific databases

CTDi 7707.
ZFINi ZDB-GENE-030131-8769. znf148.

Phylogenomic databases

eggNOGi COG5048.
GeneTreei ENSGT00530000063238.
HOGENOMi HOG000293204.
HOVERGENi HBG054247.
InParanoidi A0MS83.
OMAi NDAYLNN.
OrthoDBi EOG76QFGM.
PhylomeDBi A0MS83.
TreeFami TF331779.

Miscellaneous databases

NextBioi 20883373.
PROi A0MS83.

Gene expression databases

Bgeei A0MS83.

Family and domain databases

Gene3Di 3.30.160.60. 4 hits.
InterProi IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 4 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcription factor ZBP-89 controls generation of the hematopoietic lineage in zebrafish and mouse embryonic stem cells."
    Li X., Xiong J.-W., Shelley C.S., Park H., Arnaout M.A.
    Development 133:3641-3650(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
    Tissue: Embryo.
  2. "The zebrafish reference genome sequence and its relationship to the human genome."
    Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
    , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
    Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tuebingen.
  3. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiZN148_DANRE
AccessioniPrimary (citable) accession number: A0MS83
Secondary accession number(s): B0S5L9, B3DIY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: November 25, 2008
Last modified: October 29, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Knock-down of the znf148 mRNA disrupts both primitive and definitive hematopoiesis, but does not affect primary blood vessel formation. Most embryos die before four days after fertilization.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3