A0MS83 (ZN148_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein 148

Alternative name(s):
Transcription factor ZBP-89
Zinc finger DNA-binding protein 89

Gene names

Name:	znf148
Synonyms:	zbp89
ORF Names:	si:dkey-101k6.6

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	808 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in transcriptional regulation. Represses the transcription of a number of genes. Required for primitive and definitive hematopoiesis during embryonic development. Ref.1
Subcellular location	Nucleus By similarity.
Developmental stage	Detected in posterior lateral mesoderm, head mesenchyme and the intermediate cell mass in embryos 24 hours after fertilization. Ref.1
Miscellaneous	Knock-down of the znf148 mRNA disrupts both primitive and definitive hematopoiesis, but does not affect primary blood vessel formation. Most embryos die before four days after fertilization.
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 4 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	hemopoiesis Inferred from mutant phenotype Ref.1PubMed 18250154. Source: ZFIN regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 808	808	Zinc finger protein 148		PRO_0000354690
Regions
	Zinc finger	180 – 202	23	C2H2-type 1
	Zinc finger	208 – 230	23	C2H2-type 2
	Zinc finger	236 – 258	23	C2H2-type 3
	Zinc finger	264 – 287	24	C2H2-type 4
	Compositional bias	58 – 87	30	Asp/Glu-rich
	Compositional bias	151 – 156	6	Poly-Lys
Experimental info
	Sequence conflict	66	1	E → DD in AAI63289. Ref.3
	Sequence conflict	325	1	K → T in ABK40532. Ref.1
	Sequence conflict	429	1	Q → E in AAI63289. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	A0MS83 [UniParc]. Last modified November 25, 2008. Version 2. Checksum: B87251E494294A77	FASTA	808	90,507
	10 20 30 40 50 60 MNIDDKLEGM LLKYSPVGLQ HPSRGLGPSR VDGRGRRGSL DMTLGERSLA NHPLLAEDDD 70 80 90 100 110 120 DEDEEEDDDD DLAGEGLTSH DLISQDDLMV HEETVKNDGQ DEAAFSHRIS NKLHYTLSMP 130 140 150 160 170 180 VNIKQEMKVP DSLILNKKEK KPGRDPSDCH KKKKRKQRSP AKILTINEDG SLGHQNPKSH 190 200 210 220 230 240 ICEHCNAAFR TNYHLQRHVF IHTGEKPFQC NQCDMRFIQK YLLQRHEKIH TGEKPFRCDE 250 260 270 280 290 300 CGMKFIQKYH MERHKRTHSG EKPYQCDYCH QFFSRTDRVL KHRRMCHENK DRKVQKTAVK 310 320 330 340 350 360 DSPLRTPENL GFSFPAKDCT LPKKKRQKTS DKSRASITNP AVDKVVEADM DEKTEDRLAK 370 380 390 400 410 420 SECLPLYAVA TKVKDEYVVT DYSVELPDSP PGSRHLAGEE SNDEIISPPK LVLKKIASRR 430 440 450 460 470 480 GFKQQALEQS QSLSPLSSFE ESKVTRYTFE IVDNKGLLDV ETNPDMESVE ALQVGQAKPT 490 500 510 520 530 540 GSSTNYDDAM QFVKKRRYLQ AATANTSRDY ALNVSSIVSQ SSVTQASVAT VIDETVPATI 550 560 570 580 590 600 LSETQTLNVE IKSSNEKNVL PDEVLQTLLD HYSNKANGQA EISFSVADTE VTSSISINSS 610 620 630 640 650 660 DESSPAEALV TSSQAPPTEK ASLLQEYSKF LQQALERTSQ NDTYLNNQSL TFVNDSASLA 670 680 690 700 710 720 GQPLFSTEKQ FTSPPRFRPT MNSPLRSTLE KPNFNLLVDT QHSFSFSGDE TNPSSVSPTE 730 740 750 760 770 780 DFLDQVTSPK KTDPQSISQT FQITTFDQNF RSQFPSSRSG ISPQFSIASG QVTLRGHGGA 790 800 DFPEFSLVNE TRTQLTSSPD ATSSQTFG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcription factor ZBP-89 controls generation of the hematopoietic lineage in zebrafish and mouse embryonic stem cells." Li X., Xiong J.-W., Shelley C.S., Park H., Arnaout M.A. Development 133:3641-3650(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE. Tissue: Embryo.
[2]	The Danio rerio sequencing project at the Sanger Institute Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[3]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EF032416 mRNA. Translation: ABK40532.1. BX255930 Genomic DNA. Translation: CAQ14829.1. BC163289 mRNA. Translation: AAI63289.1.
IPI	IPI00844872.
RefSeq	NP_001073649.1. NM_001080180.1.
UniGene	Dr.76709.
3D structure databases
ProteinModelPortal	A0MS83.
SMR	A0MS83. Positions 233-263.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000077389; ENSDARP00000071856; ENSDARG00000055106.
GeneID	560300.
KEGG	dre:560300.
Organism-specific databases
CTD	7707.
ZFIN	ZDB-GENE-030131-8769. znf148.
Phylogenomic databases
eggNOG	COG5048.
GeneTree	ENSGT00530000063238.
HOGENOM	HOG000293204.
HOVERGEN	HBG054247.
InParanoid	A0MS83.
OMA	NDAYLNN.
OrthoDB	EOG43N7C2.
Gene expression databases
ArrayExpress	A0MS83.
Bgee	A0MS83.
Family and domain databases
Gene3D	3.30.160.60. 4 hits.
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
SMART	SM00355. ZnF_C2H2. 4 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 4 hits. PS50157. ZINC_FINGER_C2H2_2. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	20883373.

Entry information

Entry name

ZN148_DANRE

Accession

Primary (citable) accession number: A0MS83
Secondary accession number(s): B0S5L9, B3DIY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	November 25, 2008
Last modified:	April 3, 2013
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program