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A0MS83

- ZN148_DANRE

UniProt

A0MS83 - ZN148_DANRE

Protein

Zinc finger protein 148

Gene

znf148

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 2 (25 Nov 2008)
      Previous versions | rss
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    Functioni

    Involved in transcriptional regulation. Represses the transcription of a number of genes. Required for primitive and definitive hematopoiesis during embryonic development.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri180 – 20223C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri208 – 23023C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri236 – 25823C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri264 – 28724C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. hemopoiesis Source: ZFIN
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger protein 148
    Alternative name(s):
    Transcription factor ZBP-89
    Zinc finger DNA-binding protein 89
    Gene namesi
    Name:znf148
    Synonyms:zbp89
    ORF Names:si:dkey-101k6.6
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Chromosome 9

    Organism-specific databases

    ZFINiZDB-GENE-030131-8769. znf148.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 808808Zinc finger protein 148PRO_0000354690Add
    BLAST

    Expressioni

    Developmental stagei

    Detected in posterior lateral mesoderm, head mesenchyme and the intermediate cell mass in embryos 24 hours after fertilization.1 Publication

    Gene expression databases

    BgeeiA0MS83.

    Structurei

    3D structure databases

    ProteinModelPortaliA0MS83.
    SMRiA0MS83. Positions 233-263.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi58 – 8730Asp/Glu-richAdd
    BLAST
    Compositional biasi151 – 1566Poly-Lys

    Sequence similaritiesi

    Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri180 – 20223C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri208 – 23023C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri236 – 25823C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri264 – 28724C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    GeneTreeiENSGT00530000063238.
    HOGENOMiHOG000293204.
    HOVERGENiHBG054247.
    InParanoidiA0MS83.
    OMAiNDAYLNN.
    OrthoDBiEOG76QFGM.
    PhylomeDBiA0MS83.
    TreeFamiTF331779.

    Family and domain databases

    Gene3Di3.30.160.60. 4 hits.
    InterProiIPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 4 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
    PS50157. ZINC_FINGER_C2H2_2. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0MS83-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNIDDKLEGM LLKYSPVGLQ HPSRGLGPSR VDGRGRRGSL DMTLGERSLA    50
    NHPLLAEDDD DEDEEEDDDD DLAGEGLTSH DLISQDDLMV HEETVKNDGQ 100
    DEAAFSHRIS NKLHYTLSMP VNIKQEMKVP DSLILNKKEK KPGRDPSDCH 150
    KKKKRKQRSP AKILTINEDG SLGHQNPKSH ICEHCNAAFR TNYHLQRHVF 200
    IHTGEKPFQC NQCDMRFIQK YLLQRHEKIH TGEKPFRCDE CGMKFIQKYH 250
    MERHKRTHSG EKPYQCDYCH QFFSRTDRVL KHRRMCHENK DRKVQKTAVK 300
    DSPLRTPENL GFSFPAKDCT LPKKKRQKTS DKSRASITNP AVDKVVEADM 350
    DEKTEDRLAK SECLPLYAVA TKVKDEYVVT DYSVELPDSP PGSRHLAGEE 400
    SNDEIISPPK LVLKKIASRR GFKQQALEQS QSLSPLSSFE ESKVTRYTFE 450
    IVDNKGLLDV ETNPDMESVE ALQVGQAKPT GSSTNYDDAM QFVKKRRYLQ 500
    AATANTSRDY ALNVSSIVSQ SSVTQASVAT VIDETVPATI LSETQTLNVE 550
    IKSSNEKNVL PDEVLQTLLD HYSNKANGQA EISFSVADTE VTSSISINSS 600
    DESSPAEALV TSSQAPPTEK ASLLQEYSKF LQQALERTSQ NDTYLNNQSL 650
    TFVNDSASLA GQPLFSTEKQ FTSPPRFRPT MNSPLRSTLE KPNFNLLVDT 700
    QHSFSFSGDE TNPSSVSPTE DFLDQVTSPK KTDPQSISQT FQITTFDQNF 750
    RSQFPSSRSG ISPQFSIASG QVTLRGHGGA DFPEFSLVNE TRTQLTSSPD 800
    ATSSQTFG 808
    Length:808
    Mass (Da):90,507
    Last modified:November 25, 2008 - v2
    Checksum:iB87251E494294A77
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti66 – 661E → DD in AAI63289. 1 PublicationCurated
    Sequence conflicti325 – 3251K → T in ABK40532. (PubMed:16914492)Curated
    Sequence conflicti429 – 4291Q → E in AAI63289. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF032416 mRNA. Translation: ABK40532.1.
    BX255930 Genomic DNA. Translation: CAQ14829.1.
    BC163289 mRNA. Translation: AAI63289.1.
    RefSeqiNP_001073649.1. NM_001080180.1.
    UniGeneiDr.76709.

    Genome annotation databases

    EnsembliENSDART00000077389; ENSDARP00000071856; ENSDARG00000055106.
    GeneIDi560300.
    KEGGidre:560300.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF032416 mRNA. Translation: ABK40532.1 .
    BX255930 Genomic DNA. Translation: CAQ14829.1 .
    BC163289 mRNA. Translation: AAI63289.1 .
    RefSeqi NP_001073649.1. NM_001080180.1.
    UniGenei Dr.76709.

    3D structure databases

    ProteinModelPortali A0MS83.
    SMRi A0MS83. Positions 233-263.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSDART00000077389 ; ENSDARP00000071856 ; ENSDARG00000055106 .
    GeneIDi 560300.
    KEGGi dre:560300.

    Organism-specific databases

    CTDi 7707.
    ZFINi ZDB-GENE-030131-8769. znf148.

    Phylogenomic databases

    eggNOGi COG5048.
    GeneTreei ENSGT00530000063238.
    HOGENOMi HOG000293204.
    HOVERGENi HBG054247.
    InParanoidi A0MS83.
    OMAi NDAYLNN.
    OrthoDBi EOG76QFGM.
    PhylomeDBi A0MS83.
    TreeFami TF331779.

    Miscellaneous databases

    NextBioi 20883373.
    PROi A0MS83.

    Gene expression databases

    Bgeei A0MS83.

    Family and domain databases

    Gene3Di 3.30.160.60. 4 hits.
    InterProi IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 4 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 4 hits.
    PS50157. ZINC_FINGER_C2H2_2. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcription factor ZBP-89 controls generation of the hematopoietic lineage in zebrafish and mouse embryonic stem cells."
      Li X., Xiong J.-W., Shelley C.S., Park H., Arnaout M.A.
      Development 133:3641-3650(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
      Tissue: Embryo.
    2. "The zebrafish reference genome sequence and its relationship to the human genome."
      Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
      , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
      Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Tuebingen.
    3. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiZN148_DANRE
    AccessioniPrimary (citable) accession number: A0MS83
    Secondary accession number(s): B0S5L9, B3DIY1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 25, 2008
    Last sequence update: November 25, 2008
    Last modified: October 1, 2014
    This is version 61 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Knock-down of the znf148 mRNA disrupts both primitive and definitive hematopoiesis, but does not affect primary blood vessel formation. Most embryos die before four days after fertilization.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3