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A0MS83 (ZN148_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein 148
Alternative name(s):
Transcription factor ZBP-89
Zinc finger DNA-binding protein 89
Gene names
Name:znf148
Synonyms:zbp89
ORF Names:si:dkey-101k6.6
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length808 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in transcriptional regulation. Represses the transcription of a number of genes. Required for primitive and definitive hematopoiesis during embryonic development. Ref.1

Subcellular location

Nucleus By similarity.

Developmental stage

Detected in posterior lateral mesoderm, head mesenchyme and the intermediate cell mass in embryos 24 hours after fertilization. Ref.1

Miscellaneous

Knock-down of the znf148 mRNA disrupts both primitive and definitive hematopoiesis, but does not affect primary blood vessel formation. Most embryos die before four days after fertilization.

Sequence similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.

Contains 4 C2H2-type zinc fingers.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionRepressor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processhemopoiesis

Inferred from mutant phenotype Ref.1PubMed 18250154. Source: ZFIN

regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 808808Zinc finger protein 148
PRO_0000354690

Regions

Zinc finger180 – 20223C2H2-type 1
Zinc finger208 – 23023C2H2-type 2
Zinc finger236 – 25823C2H2-type 3
Zinc finger264 – 28724C2H2-type 4
Compositional bias58 – 8730Asp/Glu-rich
Compositional bias151 – 1566Poly-Lys

Experimental info

Sequence conflict661E → DD in AAI63289. Ref.3
Sequence conflict3251K → T in ABK40532. Ref.1
Sequence conflict4291Q → E in AAI63289. Ref.3

Sequences

Sequence LengthMass (Da)Tools
A0MS83 [UniParc].

Last modified November 25, 2008. Version 2.
Checksum: B87251E494294A77

FASTA80890,507
        10         20         30         40         50         60 
MNIDDKLEGM LLKYSPVGLQ HPSRGLGPSR VDGRGRRGSL DMTLGERSLA NHPLLAEDDD 

        70         80         90        100        110        120 
DEDEEEDDDD DLAGEGLTSH DLISQDDLMV HEETVKNDGQ DEAAFSHRIS NKLHYTLSMP 

       130        140        150        160        170        180 
VNIKQEMKVP DSLILNKKEK KPGRDPSDCH KKKKRKQRSP AKILTINEDG SLGHQNPKSH 

       190        200        210        220        230        240 
ICEHCNAAFR TNYHLQRHVF IHTGEKPFQC NQCDMRFIQK YLLQRHEKIH TGEKPFRCDE 

       250        260        270        280        290        300 
CGMKFIQKYH MERHKRTHSG EKPYQCDYCH QFFSRTDRVL KHRRMCHENK DRKVQKTAVK 

       310        320        330        340        350        360 
DSPLRTPENL GFSFPAKDCT LPKKKRQKTS DKSRASITNP AVDKVVEADM DEKTEDRLAK 

       370        380        390        400        410        420 
SECLPLYAVA TKVKDEYVVT DYSVELPDSP PGSRHLAGEE SNDEIISPPK LVLKKIASRR 

       430        440        450        460        470        480 
GFKQQALEQS QSLSPLSSFE ESKVTRYTFE IVDNKGLLDV ETNPDMESVE ALQVGQAKPT 

       490        500        510        520        530        540 
GSSTNYDDAM QFVKKRRYLQ AATANTSRDY ALNVSSIVSQ SSVTQASVAT VIDETVPATI 

       550        560        570        580        590        600 
LSETQTLNVE IKSSNEKNVL PDEVLQTLLD HYSNKANGQA EISFSVADTE VTSSISINSS 

       610        620        630        640        650        660 
DESSPAEALV TSSQAPPTEK ASLLQEYSKF LQQALERTSQ NDTYLNNQSL TFVNDSASLA 

       670        680        690        700        710        720 
GQPLFSTEKQ FTSPPRFRPT MNSPLRSTLE KPNFNLLVDT QHSFSFSGDE TNPSSVSPTE 

       730        740        750        760        770        780 
DFLDQVTSPK KTDPQSISQT FQITTFDQNF RSQFPSSRSG ISPQFSIASG QVTLRGHGGA 

       790        800 
DFPEFSLVNE TRTQLTSSPD ATSSQTFG 

« Hide

References

« Hide 'large scale' references
[1]"The transcription factor ZBP-89 controls generation of the hematopoietic lineage in zebrafish and mouse embryonic stem cells."
Li X., Xiong J.-W., Shelley C.S., Park H., Arnaout M.A.
Development 133:3641-3650(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
Tissue: Embryo.
[2]"The zebrafish reference genome sequence and its relationship to the human genome."
Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J. expand/collapse author list , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Tuebingen.
[3]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF032416 mRNA. Translation: ABK40532.1.
BX255930 Genomic DNA. Translation: CAQ14829.1.
BC163289 mRNA. Translation: AAI63289.1.
RefSeqNP_001073649.1. NM_001080180.1.
UniGeneDr.76709.

3D structure databases

ProteinModelPortalA0MS83.
SMRA0MS83. Positions 233-263.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSDART00000077389; ENSDARP00000071856; ENSDARG00000055106.
GeneID560300.
KEGGdre:560300.

Organism-specific databases

CTD7707.
ZFINZDB-GENE-030131-8769. znf148.

Phylogenomic databases

eggNOGCOG5048.
GeneTreeENSGT00530000063238.
HOGENOMHOG000293204.
HOVERGENHBG054247.
InParanoidA0MS83.
OMANDAYLNN.
OrthoDBEOG76QFGM.
PhylomeDBA0MS83.
TreeFamTF331779.

Gene expression databases

BgeeA0MS83.

Family and domain databases

Gene3D3.30.160.60. 4 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 4 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio20883373.
PROA0MS83.

Entry information

Entry nameZN148_DANRE
AccessionPrimary (citable) accession number: A0MS83
Secondary accession number(s): B0S5L9, B3DIY1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: November 25, 2008
Last modified: April 16, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families