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A0MQH0 (DICER_CRIGR) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoribonuclease Dicer

EC=3.1.26.3
Gene names
Name:DICER1
Synonyms:DICER
OrganismCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifier10029 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Protein attributes

Sequence length1917 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes By similarity.

Catalytic activity

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Subunit structure

Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2; DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with DHX9, AGO1, PIWIL1 and PRKRA. Associates with the 60S ribosome. Interacts with BCDIN3D By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the helicase family. Dicer subfamily.

Contains 1 Dicer dsRNA-binding fold domain.

Contains 1 DRBM (double-stranded RNA-binding) domain.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 1 PAZ domain.

Contains 2 RNase III domains.

Caution

It is uncertain whether Met-1 or Met-11 is the initiator.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19171917Endoribonuclease Dicer
PRO_0000373983

Regions

Domain51 – 227177Helicase ATP-binding
Domain433 – 602170Helicase C-terminal
Domain630 – 72293Dicer dsRNA-binding fold
Domain898 – 1042145PAZ
Domain1276 – 1403128RNase III 1
Domain1661 – 1819159RNase III 2
Domain1844 – 190966DRBM
Nucleotide binding64 – 718ATP By similarity
Region256 – 595340Required for interaction with PRKRA and TARBP2 By similarity
Motif175 – 1784DECH box

Sites

Metal binding13161Magnesium or manganese 1 By similarity
Metal binding13951Magnesium or manganese 1 By similarity
Metal binding13981Magnesium or manganese 1 By similarity
Metal binding17001Magnesium or manganese 2 By similarity
Metal binding18051Magnesium or manganese 2 By similarity
Metal binding18081Magnesium or manganese 2 By similarity
Site18011Important for activity By similarity

Amino acid modifications

Modified residue4131Phosphoserine By similarity
Modified residue4151Phosphoserine By similarity
Modified residue10161Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
A0MQH0 [UniParc].

Last modified September 1, 2009. Version 3.
Checksum: E42D6F5412E65A0F

FASTA1,917217,171
        10         20         30         40         50         60 
MKSPALQPLS MAGLQLMTPA SSPMGPFFGL PWQQEAIHDN IYTPRKYQVE LLEAALDHNT 

        70         80         90        100        110        120 
IVCLNTGSGK TFIAVLLTKE LAHQIRGDLN PRAKRTVFLV NSANQVAQQV SAVRTHSDLK 

       130        140        150        160        170        180 
VGEYSNLEVN ASWTKERWSQ EFTKHQVLIM TCYVALNVLK NGYLSLSDIN LLVFDECHLA 

       190        200        210        220        230        240 
ILDHPYREIM KLCESCPSCP RILGLTASIL NGKCDPEELE EKIQKLEKIL KSNAETATDL 

       250        260        270        280        290        300 
VVLDRYASQP CEIVVDCGPF TDRSGLYERL LMELEEAINF INDCNVSVHS KERDSTLISK 

       310        320        330        340        350        360 
QILSDCRAVL VVLGPWCADK VAGMMVRELQ KYIKHEQEEL HRKFLLFTDT LLRKIHALCE 

       370        380        390        400        410        420 
EHFSPASLDL KYVTPKVMKL LEILRKYKPY ERQQFESVEW YNNRNQDNYV SWSDSEDDDD 

       430        440        450        460        470        480 
DEEIEEKEKP ETNFPSPFTN ILCGIIFVER RYTAVVLNRL IKEAGKQDPE LAYISSNFIT 

       490        500        510        520        530        540 
GHGIGKNQPR SKQMEAEFRK QEEVLRKFRA HETNLLIATS VVEEGVDIPK CNLVVRFDLP 

       550        560        570        580        590        600 
TEYRSYVQSK GRARAPISNY VMLADTDKIQ SFEEDLKTYK AIEKILRNKC SKSVDGAEAD 

       610        620        630        640        650        660 
VDAVVDDDDV FPPYVLRPDD GGPRVTINTA IGHVNRYCAR LPSDPFTHLA PKCRTQELPD 

       670        680        690        700        710        720 
GTFYSTLYLP INSPLRASIV GPPMGCVRLA ERVVALICCE KLHKIGELDE HLMPVGKETV 

       730        740        750        760        770        780 
KYEEELDLHD EEETSVPGRP GSTKRRQCYP KAIPECLRES YPKPDQPCYL YVIGMVLTTP 

       790        800        810        820        830        840 
LPDELNFRRR KLYPPEDTTR CFGILTAKPI PQIPHFPVYT RSGEVTISIE LKKSGFTLSQ 

       850        860        870        880        890        900 
QMLELITRLH QYIFSHILRL EKPALEFKPT GAESAYCVLP LNVVNDSSTL DIDFTFMEDI 

       910        920        930        940        950        960 
EKSEARIGIP STKYSKETPF VFKLEDYQDA VIIPRYRNFD QPHRFYVADV YTDLTPLSKF 

       970        980        990       1000       1010       1020 
PSPEYETFAE YYKTKYNLDL TNLNQPLLDV DHTSSRLNLL TPRHLNQKGK ALPLSSAEKR 

      1030       1040       1050       1060       1070       1080 
KAKWESLQNK QILVPELCAI HPIPASLWRK AVCLPSILYR LHCLLTAEEL RAQTASDAGV 

      1090       1100       1110       1120       1130       1140 
GVRSLPADFR YPNLDFGWKK SIDSKSFIST CNSSLAENDN YCKHSTIIVP ENAAHQGATR 

      1150       1160       1170       1180       1190       1200 
TPLENHDQMS VNCRRLPAES SGKLQIEVST DLTAINGLSY NKNLANGSYD LVNRDFCQGN 

      1210       1220       1230       1240       1250       1260 
QLNYFKQEIP VQPTTSYPIQ NLYHYENQPK PSNECTLLSN KYLDGNANTS TSDGSPAVST 

      1270       1280       1290       1300       1310       1320 
MPAVMSAGRA LKDRMDSEQS PSAGYSSRTL GPNPGLILQA LTLSNASDGF NLERLEMLGD 

      1330       1340       1350       1360       1370       1380 
SFLKHAITTY LFCTYPDAHE GRLSYMRSKK VSNCNLYRLG KKKGLPSRMV VSIFDPPVNW 

      1390       1400       1410       1420       1430       1440 
LPPGYVVNQD KSNAEKWEKD EMTKDCLLAN GKLGKDCEEE EALTWRAPKE EAEYEDDFLE 

      1450       1460       1470       1480       1490       1500 
YDQEHIQFID SMLMGSGAFV KKISLSPFST SDSAYEWKMP KKSSLGSMPF SSDLEDFDYS 

      1510       1520       1530       1540       1550       1560 
SWDAMCYLDP SKAVEEDDFV VGFWNPSEEN CGVDTGKQSI SYDLHTEQCI ADKSIADCVE 

      1570       1580       1590       1600       1610       1620 
ALLGCYLTSC GERAAQLFLC SLGLKVLPVI KRTSREKALY PAQENFSSQQ KSLSGSCAAT 

      1630       1640       1650       1660       1670       1680 
AASPRSSAGK DLEYGCLKIP PRCMFDHPDA EKTLNHLISG FENFEKKINY RFKNKAYLLQ 

      1690       1700       1710       1720       1730       1740 
AFTHASYHYN TITDCYQRLE FLGDAILDYL ITKHLYEDPR QHSPGVLTDL RSALVNNTIF 

      1750       1760       1770       1780       1790       1800 
ASLAVKYDYH KYFKAVSPEL FHVIDDFVQF QLEKNEMQGM DSELRRSEED EEKEEDIEVP 

      1810       1820       1830       1840       1850       1860 
KAMGDIFESL AGAIYMDSGM SLEVVWQVYY PMMRPLIEKF SANVPRSPVR ELLEMEPETA 

      1870       1880       1890       1900       1910 
KFSPAERTYD GKVRVTVEVV GKGKFKGVGR SYRIAKSAAA RRALRSLKAN QPQVPNS 

« Hide

References

[1]Wallerstorfer D.
Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF031271 mRNA. Translation: ABK28790.1.
RefSeqNP_001231198.1. NM_001244269.1.
XP_007647243.1. XM_007649053.1.
XP_007647244.1. XM_007649054.1.

3D structure databases

ProteinModelPortalA0MQH0.
SMRA0MQH0. Positions 1649-1911.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100689239.
KEGGcge:100689239.

Organism-specific databases

CTD23405.

Phylogenomic databases

KOK11592.

Family and domain databases

Gene3D1.10.1520.10. 4 hits.
3.30.160.20. 1 hit.
3.40.50.300. 2 hits.
InterProIPR005034. Dicer_dimerisation_dom.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014720. dsRNA-bd_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR003100. PAZ_dom.
IPR000999. RNase_III_dom.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF02170. PAZ. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00358. DSRM. 1 hit.
SM00490. HELICc. 1 hit.
SM00949. PAZ. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMSSF101690. SSF101690. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 4 hits.
PROSITEPS51327. DICER_DSRBF. 1 hit.
PS50137. DS_RBD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50821. PAZ. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDICER_CRIGR
AccessionPrimary (citable) accession number: A0MQH0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 1, 2009
Last modified: July 9, 2014
This is version 48 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families