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A0MQH0

- DICER_CRIGR

UniProt

A0MQH0 - DICER_CRIGR

Protein

Endoribonuclease Dicer

Gene

DICER1

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 3 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes By similarity.By similarity

    Catalytic activityi

    Endonucleolytic cleavage to 5'-phosphomonoester.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi1316 – 13161Magnesium or manganese 1By similarity
    Metal bindingi1395 – 13951Magnesium or manganese 1By similarity
    Metal bindingi1398 – 13981Magnesium or manganese 1By similarity
    Metal bindingi1700 – 17001Magnesium or manganese 2By similarity
    Sitei1801 – 18011Important for activityBy similarity
    Metal bindingi1805 – 18051Magnesium or manganese 2By similarity
    Metal bindingi1808 – 18081Magnesium or manganese 2By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi64 – 718ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. helicase activity Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW
    4. ribonuclease III activity Source: UniProtKB-EC
    5. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. pre-miRNA processing Source: UniProtKB
    2. production of siRNA involved in RNA interference Source: UniProtKB
    3. targeting of mRNA for destruction involved in RNA interference Source: UniProtKB

    Keywords - Molecular functioni

    Endonuclease, Helicase, Hydrolase, Nuclease

    Keywords - Biological processi

    RNA-mediated gene silencing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoribonuclease Dicer (EC:3.1.26.3)
    Gene namesi
    Name:DICER1
    Synonyms:DICER
    OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
    Taxonomic identifieri10029 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. RISC complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 19171917Endoribonuclease DicerPRO_0000373983Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei413 – 4131PhosphoserineBy similarity
    Modified residuei415 – 4151PhosphoserineBy similarity
    Modified residuei1016 – 10161PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2; DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with DHX9, AGO1, PIWIL1 and PRKRA. Associates with the 60S ribosome. Interacts with BCDIN3D By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliA0MQH0.
    SMRiA0MQH0. Positions 1649-1911.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini51 – 227177Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini433 – 602170Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini630 – 72293Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd
    BLAST
    Domaini898 – 1042145PAZPROSITE-ProRule annotationAdd
    BLAST
    Domaini1276 – 1403128RNase III 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1661 – 1819159RNase III 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1844 – 190966DRBMPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni256 – 595340Required for interaction with PRKRA and TARBP2By similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi175 – 1784DECH box

    Sequence similaritiesi

    Belongs to the helicase family. Dicer subfamily.PROSITE-ProRule annotation
    Contains 1 Dicer dsRNA-binding fold domain.PROSITE-ProRule annotation
    Contains 1 DRBM (double-stranded RNA-binding) domain.PROSITE-ProRule annotation
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
    Contains 1 PAZ domain.PROSITE-ProRule annotation
    Contains 2 RNase III domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    KOiK11592.

    Family and domain databases

    Gene3Di1.10.1520.10. 4 hits.
    3.30.160.20. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR005034. Dicer_dimerisation_dom.
    IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014720. dsRNA-bd_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR003100. PAZ_dom.
    IPR000999. RNase_III_dom.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF03368. Dicer_dimer. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF02170. PAZ. 1 hit.
    PF00636. Ribonuclease_3. 2 hits.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00358. DSRM. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00949. PAZ. 1 hit.
    SM00535. RIBOc. 2 hits.
    [Graphical view]
    SUPFAMiSSF101690. SSF101690. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF69065. SSF69065. 4 hits.
    PROSITEiPS51327. DICER_DSRBF. 1 hit.
    PS50137. DS_RBD. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS50821. PAZ. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0MQH0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSPALQPLS MAGLQLMTPA SSPMGPFFGL PWQQEAIHDN IYTPRKYQVE     50
    LLEAALDHNT IVCLNTGSGK TFIAVLLTKE LAHQIRGDLN PRAKRTVFLV 100
    NSANQVAQQV SAVRTHSDLK VGEYSNLEVN ASWTKERWSQ EFTKHQVLIM 150
    TCYVALNVLK NGYLSLSDIN LLVFDECHLA ILDHPYREIM KLCESCPSCP 200
    RILGLTASIL NGKCDPEELE EKIQKLEKIL KSNAETATDL VVLDRYASQP 250
    CEIVVDCGPF TDRSGLYERL LMELEEAINF INDCNVSVHS KERDSTLISK 300
    QILSDCRAVL VVLGPWCADK VAGMMVRELQ KYIKHEQEEL HRKFLLFTDT 350
    LLRKIHALCE EHFSPASLDL KYVTPKVMKL LEILRKYKPY ERQQFESVEW 400
    YNNRNQDNYV SWSDSEDDDD DEEIEEKEKP ETNFPSPFTN ILCGIIFVER 450
    RYTAVVLNRL IKEAGKQDPE LAYISSNFIT GHGIGKNQPR SKQMEAEFRK 500
    QEEVLRKFRA HETNLLIATS VVEEGVDIPK CNLVVRFDLP TEYRSYVQSK 550
    GRARAPISNY VMLADTDKIQ SFEEDLKTYK AIEKILRNKC SKSVDGAEAD 600
    VDAVVDDDDV FPPYVLRPDD GGPRVTINTA IGHVNRYCAR LPSDPFTHLA 650
    PKCRTQELPD GTFYSTLYLP INSPLRASIV GPPMGCVRLA ERVVALICCE 700
    KLHKIGELDE HLMPVGKETV KYEEELDLHD EEETSVPGRP GSTKRRQCYP 750
    KAIPECLRES YPKPDQPCYL YVIGMVLTTP LPDELNFRRR KLYPPEDTTR 800
    CFGILTAKPI PQIPHFPVYT RSGEVTISIE LKKSGFTLSQ QMLELITRLH 850
    QYIFSHILRL EKPALEFKPT GAESAYCVLP LNVVNDSSTL DIDFTFMEDI 900
    EKSEARIGIP STKYSKETPF VFKLEDYQDA VIIPRYRNFD QPHRFYVADV 950
    YTDLTPLSKF PSPEYETFAE YYKTKYNLDL TNLNQPLLDV DHTSSRLNLL 1000
    TPRHLNQKGK ALPLSSAEKR KAKWESLQNK QILVPELCAI HPIPASLWRK 1050
    AVCLPSILYR LHCLLTAEEL RAQTASDAGV GVRSLPADFR YPNLDFGWKK 1100
    SIDSKSFIST CNSSLAENDN YCKHSTIIVP ENAAHQGATR TPLENHDQMS 1150
    VNCRRLPAES SGKLQIEVST DLTAINGLSY NKNLANGSYD LVNRDFCQGN 1200
    QLNYFKQEIP VQPTTSYPIQ NLYHYENQPK PSNECTLLSN KYLDGNANTS 1250
    TSDGSPAVST MPAVMSAGRA LKDRMDSEQS PSAGYSSRTL GPNPGLILQA 1300
    LTLSNASDGF NLERLEMLGD SFLKHAITTY LFCTYPDAHE GRLSYMRSKK 1350
    VSNCNLYRLG KKKGLPSRMV VSIFDPPVNW LPPGYVVNQD KSNAEKWEKD 1400
    EMTKDCLLAN GKLGKDCEEE EALTWRAPKE EAEYEDDFLE YDQEHIQFID 1450
    SMLMGSGAFV KKISLSPFST SDSAYEWKMP KKSSLGSMPF SSDLEDFDYS 1500
    SWDAMCYLDP SKAVEEDDFV VGFWNPSEEN CGVDTGKQSI SYDLHTEQCI 1550
    ADKSIADCVE ALLGCYLTSC GERAAQLFLC SLGLKVLPVI KRTSREKALY 1600
    PAQENFSSQQ KSLSGSCAAT AASPRSSAGK DLEYGCLKIP PRCMFDHPDA 1650
    EKTLNHLISG FENFEKKINY RFKNKAYLLQ AFTHASYHYN TITDCYQRLE 1700
    FLGDAILDYL ITKHLYEDPR QHSPGVLTDL RSALVNNTIF ASLAVKYDYH 1750
    KYFKAVSPEL FHVIDDFVQF QLEKNEMQGM DSELRRSEED EEKEEDIEVP 1800
    KAMGDIFESL AGAIYMDSGM SLEVVWQVYY PMMRPLIEKF SANVPRSPVR 1850
    ELLEMEPETA KFSPAERTYD GKVRVTVEVV GKGKFKGVGR SYRIAKSAAA 1900
    RRALRSLKAN QPQVPNS 1917
    Length:1,917
    Mass (Da):217,171
    Last modified:September 1, 2009 - v3
    Checksum:iE42D6F5412E65A0F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF031271 mRNA. Translation: ABK28790.1.
    RefSeqiNP_001231198.1. NM_001244269.1.
    XP_007647243.1. XM_007649053.1.
    XP_007647244.1. XM_007649054.1.

    Genome annotation databases

    GeneIDi100689239.
    KEGGicge:100689239.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF031271 mRNA. Translation: ABK28790.1 .
    RefSeqi NP_001231198.1. NM_001244269.1.
    XP_007647243.1. XM_007649053.1.
    XP_007647244.1. XM_007649054.1.

    3D structure databases

    ProteinModelPortali A0MQH0.
    SMRi A0MQH0. Positions 1649-1911.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100689239.
    KEGGi cge:100689239.

    Organism-specific databases

    CTDi 23405.

    Phylogenomic databases

    KOi K11592.

    Family and domain databases

    Gene3Di 1.10.1520.10. 4 hits.
    3.30.160.20. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR005034. Dicer_dimerisation_dom.
    IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014720. dsRNA-bd_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR003100. PAZ_dom.
    IPR000999. RNase_III_dom.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF03368. Dicer_dimer. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF02170. PAZ. 1 hit.
    PF00636. Ribonuclease_3. 2 hits.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00358. DSRM. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00949. PAZ. 1 hit.
    SM00535. RIBOc. 2 hits.
    [Graphical view ]
    SUPFAMi SSF101690. SSF101690. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF69065. SSF69065. 4 hits.
    PROSITEi PS51327. DICER_DSRBF. 1 hit.
    PS50137. DS_RBD. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS50821. PAZ. 1 hit.
    PS00517. RNASE_3_1. 1 hit.
    PS50142. RNASE_3_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Wallerstorfer D.
      Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiDICER_CRIGR
    AccessioniPrimary (citable) accession number: A0MQH0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 50 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    It is uncertain whether Met-1 or Met-11 is the initiator.Curated

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3