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Protein

Endoribonuclease Dicer

Gene

DICER1

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi1316Magnesium or manganese 1By similarity1
Metal bindingi1395Magnesium or manganese 1By similarity1
Metal bindingi1398Magnesium or manganese 1By similarity1
Metal bindingi1700Magnesium or manganese 2By similarity1
Sitei1801Important for activityBy similarity1
Metal bindingi1805Magnesium or manganese 2By similarity1
Metal bindingi1808Magnesium or manganese 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi64 – 71ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

RNA-mediated gene silencing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endoribonuclease Dicer (EC:3.1.26.3)
Gene namesi
Name:DICER1
Synonyms:DICER
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003739831 – 1917Endoribonuclease DicerAdd BLAST1917

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei413PhosphoserineBy similarity1
Modified residuei415PhosphoserineBy similarity1
Modified residuei1016PhosphoserineBy similarity1
Modified residuei1160PhosphoserineBy similarity1
Modified residuei1456PhosphoserineBy similarity1
Modified residuei1464PhosphoserineBy similarity1
Modified residuei1466PhosphoserineBy similarity1
Modified residuei1863PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2; DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with DHX9, AGO1, PIWIL1 and PRKRA. Interacts with AGO2, TARBP2, EIF6, MOV10 and RPL7A (60S ribosome subunit); they form a large RNA-induced silencing complex (RISC). Interacts with BCDIN3D (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliA0MQH0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 227Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST177
Domaini433 – 602Helicase C-terminalPROSITE-ProRule annotationAdd BLAST170
Domaini630 – 722Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd BLAST93
Domaini898 – 1042PAZPROSITE-ProRule annotationAdd BLAST145
Domaini1276 – 1403RNase III 1PROSITE-ProRule annotationAdd BLAST128
Domaini1661 – 1819RNase III 2PROSITE-ProRule annotationAdd BLAST159
Domaini1844 – 1909DRBMPROSITE-ProRule annotationAdd BLAST66

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni256 – 595Required for interaction with PRKRA and TARBP2By similarityAdd BLAST340

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi175 – 178DECH box4

Sequence similaritiesi

Belongs to the helicase family. Dicer subfamily.PROSITE-ProRule annotation
Contains 1 Dicer dsRNA-binding fold domain.PROSITE-ProRule annotation
Contains 1 DRBM (double-stranded RNA-binding) domain.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 PAZ domain.PROSITE-ProRule annotation
Contains 2 RNase III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

KOiK11592.

Family and domain databases

CDDicd00593. RIBOc. 2 hits.
Gene3Di1.10.1520.10. 4 hits.
3.30.160.20. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR005034. Dicer_dimerisation_dom.
IPR014720. dsRBD_dom.
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR003100. PAZ_dom.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF02170. PAZ. 1 hit.
PF04851. ResIII. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00358. DSRM. 1 hit.
SM00490. HELICc. 1 hit.
SM00949. PAZ. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 4 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS50137. DS_RBD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50821. PAZ. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0MQH0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSPALQPLS MAGLQLMTPA SSPMGPFFGL PWQQEAIHDN IYTPRKYQVE
60 70 80 90 100
LLEAALDHNT IVCLNTGSGK TFIAVLLTKE LAHQIRGDLN PRAKRTVFLV
110 120 130 140 150
NSANQVAQQV SAVRTHSDLK VGEYSNLEVN ASWTKERWSQ EFTKHQVLIM
160 170 180 190 200
TCYVALNVLK NGYLSLSDIN LLVFDECHLA ILDHPYREIM KLCESCPSCP
210 220 230 240 250
RILGLTASIL NGKCDPEELE EKIQKLEKIL KSNAETATDL VVLDRYASQP
260 270 280 290 300
CEIVVDCGPF TDRSGLYERL LMELEEAINF INDCNVSVHS KERDSTLISK
310 320 330 340 350
QILSDCRAVL VVLGPWCADK VAGMMVRELQ KYIKHEQEEL HRKFLLFTDT
360 370 380 390 400
LLRKIHALCE EHFSPASLDL KYVTPKVMKL LEILRKYKPY ERQQFESVEW
410 420 430 440 450
YNNRNQDNYV SWSDSEDDDD DEEIEEKEKP ETNFPSPFTN ILCGIIFVER
460 470 480 490 500
RYTAVVLNRL IKEAGKQDPE LAYISSNFIT GHGIGKNQPR SKQMEAEFRK
510 520 530 540 550
QEEVLRKFRA HETNLLIATS VVEEGVDIPK CNLVVRFDLP TEYRSYVQSK
560 570 580 590 600
GRARAPISNY VMLADTDKIQ SFEEDLKTYK AIEKILRNKC SKSVDGAEAD
610 620 630 640 650
VDAVVDDDDV FPPYVLRPDD GGPRVTINTA IGHVNRYCAR LPSDPFTHLA
660 670 680 690 700
PKCRTQELPD GTFYSTLYLP INSPLRASIV GPPMGCVRLA ERVVALICCE
710 720 730 740 750
KLHKIGELDE HLMPVGKETV KYEEELDLHD EEETSVPGRP GSTKRRQCYP
760 770 780 790 800
KAIPECLRES YPKPDQPCYL YVIGMVLTTP LPDELNFRRR KLYPPEDTTR
810 820 830 840 850
CFGILTAKPI PQIPHFPVYT RSGEVTISIE LKKSGFTLSQ QMLELITRLH
860 870 880 890 900
QYIFSHILRL EKPALEFKPT GAESAYCVLP LNVVNDSSTL DIDFTFMEDI
910 920 930 940 950
EKSEARIGIP STKYSKETPF VFKLEDYQDA VIIPRYRNFD QPHRFYVADV
960 970 980 990 1000
YTDLTPLSKF PSPEYETFAE YYKTKYNLDL TNLNQPLLDV DHTSSRLNLL
1010 1020 1030 1040 1050
TPRHLNQKGK ALPLSSAEKR KAKWESLQNK QILVPELCAI HPIPASLWRK
1060 1070 1080 1090 1100
AVCLPSILYR LHCLLTAEEL RAQTASDAGV GVRSLPADFR YPNLDFGWKK
1110 1120 1130 1140 1150
SIDSKSFIST CNSSLAENDN YCKHSTIIVP ENAAHQGATR TPLENHDQMS
1160 1170 1180 1190 1200
VNCRRLPAES SGKLQIEVST DLTAINGLSY NKNLANGSYD LVNRDFCQGN
1210 1220 1230 1240 1250
QLNYFKQEIP VQPTTSYPIQ NLYHYENQPK PSNECTLLSN KYLDGNANTS
1260 1270 1280 1290 1300
TSDGSPAVST MPAVMSAGRA LKDRMDSEQS PSAGYSSRTL GPNPGLILQA
1310 1320 1330 1340 1350
LTLSNASDGF NLERLEMLGD SFLKHAITTY LFCTYPDAHE GRLSYMRSKK
1360 1370 1380 1390 1400
VSNCNLYRLG KKKGLPSRMV VSIFDPPVNW LPPGYVVNQD KSNAEKWEKD
1410 1420 1430 1440 1450
EMTKDCLLAN GKLGKDCEEE EALTWRAPKE EAEYEDDFLE YDQEHIQFID
1460 1470 1480 1490 1500
SMLMGSGAFV KKISLSPFST SDSAYEWKMP KKSSLGSMPF SSDLEDFDYS
1510 1520 1530 1540 1550
SWDAMCYLDP SKAVEEDDFV VGFWNPSEEN CGVDTGKQSI SYDLHTEQCI
1560 1570 1580 1590 1600
ADKSIADCVE ALLGCYLTSC GERAAQLFLC SLGLKVLPVI KRTSREKALY
1610 1620 1630 1640 1650
PAQENFSSQQ KSLSGSCAAT AASPRSSAGK DLEYGCLKIP PRCMFDHPDA
1660 1670 1680 1690 1700
EKTLNHLISG FENFEKKINY RFKNKAYLLQ AFTHASYHYN TITDCYQRLE
1710 1720 1730 1740 1750
FLGDAILDYL ITKHLYEDPR QHSPGVLTDL RSALVNNTIF ASLAVKYDYH
1760 1770 1780 1790 1800
KYFKAVSPEL FHVIDDFVQF QLEKNEMQGM DSELRRSEED EEKEEDIEVP
1810 1820 1830 1840 1850
KAMGDIFESL AGAIYMDSGM SLEVVWQVYY PMMRPLIEKF SANVPRSPVR
1860 1870 1880 1890 1900
ELLEMEPETA KFSPAERTYD GKVRVTVEVV GKGKFKGVGR SYRIAKSAAA
1910
RRALRSLKAN QPQVPNS
Length:1,917
Mass (Da):217,171
Last modified:September 1, 2009 - v3
Checksum:iE42D6F5412E65A0F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF031271 mRNA. Translation: ABK28790.1.
RefSeqiNP_001231198.1. NM_001244269.1.
XP_007647243.1. XM_007649053.1.

Genome annotation databases

GeneIDi100689239.
KEGGicge:100689239.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF031271 mRNA. Translation: ABK28790.1.
RefSeqiNP_001231198.1. NM_001244269.1.
XP_007647243.1. XM_007649053.1.

3D structure databases

ProteinModelPortaliA0MQH0.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100689239.
KEGGicge:100689239.

Organism-specific databases

CTDi23405.

Phylogenomic databases

KOiK11592.

Family and domain databases

CDDicd00593. RIBOc. 2 hits.
Gene3Di1.10.1520.10. 4 hits.
3.30.160.20. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR005034. Dicer_dimerisation_dom.
IPR014720. dsRBD_dom.
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR003100. PAZ_dom.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF02170. PAZ. 1 hit.
PF04851. ResIII. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00358. DSRM. 1 hit.
SM00490. HELICc. 1 hit.
SM00949. PAZ. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 4 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS50137. DS_RBD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50821. PAZ. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDICER_CRIGR
AccessioniPrimary (citable) accession number: A0MQH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 1, 2009
Last modified: October 5, 2016
This is version 61 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-11 is the initiator.Curated

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.