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A0MQH0

- DICER_CRIGR

UniProt

A0MQH0 - DICER_CRIGR

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Protein

Endoribonuclease Dicer

Gene
DICER1, DICER
Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes By similarity.

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1316 – 13161Magnesium or manganese 1 By similarity
Metal bindingi1395 – 13951Magnesium or manganese 1 By similarity
Metal bindingi1398 – 13981Magnesium or manganese 1 By similarity
Metal bindingi1700 – 17001Magnesium or manganese 2 By similarity
Sitei1801 – 18011Important for activity By similarity
Metal bindingi1805 – 18051Magnesium or manganese 2 By similarity
Metal bindingi1808 – 18081Magnesium or manganese 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi64 – 718ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. helicase activity Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. ribonuclease III activity Source: UniProtKB-EC
  5. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. pre-miRNA processing Source: UniProtKB
  2. production of siRNA involved in RNA interference Source: UniProtKB
  3. targeting of mRNA for destruction involved in RNA interference Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

RNA-mediated gene silencing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endoribonuclease Dicer (EC:3.1.26.3)
Gene namesi
Name:DICER1
Synonyms:DICER
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. RISC complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19171917Endoribonuclease DicerPRO_0000373983Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei413 – 4131Phosphoserine By similarity
Modified residuei415 – 4151Phosphoserine By similarity
Modified residuei1016 – 10161Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2; DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with DHX9, AGO1, PIWIL1 and PRKRA. Associates with the 60S ribosome. Interacts with BCDIN3D By similarity.

Structurei

3D structure databases

ProteinModelPortaliA0MQH0.
SMRiA0MQH0. Positions 1649-1911.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini51 – 227177Helicase ATP-bindingAdd
BLAST
Domaini433 – 602170Helicase C-terminalAdd
BLAST
Domaini630 – 72293Dicer dsRNA-binding foldAdd
BLAST
Domaini898 – 1042145PAZAdd
BLAST
Domaini1276 – 1403128RNase III 1Add
BLAST
Domaini1661 – 1819159RNase III 2Add
BLAST
Domaini1844 – 190966DRBMAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni256 – 595340Required for interaction with PRKRA and TARBP2 By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi175 – 1784DECH box

Sequence similaritiesi

Contains 1 PAZ domain.
Contains 2 RNase III domains.

Keywords - Domaini

Repeat

Phylogenomic databases

KOiK11592.

Family and domain databases

Gene3Di1.10.1520.10. 4 hits.
3.30.160.20. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR005034. Dicer_dimerisation_dom.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014720. dsRNA-bd_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR003100. PAZ_dom.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF02170. PAZ. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00358. DSRM. 1 hit.
SM00490. HELICc. 1 hit.
SM00949. PAZ. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF101690. SSF101690. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 4 hits.
PROSITEiPS51327. DICER_DSRBF. 1 hit.
PS50137. DS_RBD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50821. PAZ. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0MQH0-1 [UniParc]FASTAAdd to Basket

« Hide

MKSPALQPLS MAGLQLMTPA SSPMGPFFGL PWQQEAIHDN IYTPRKYQVE     50
LLEAALDHNT IVCLNTGSGK TFIAVLLTKE LAHQIRGDLN PRAKRTVFLV 100
NSANQVAQQV SAVRTHSDLK VGEYSNLEVN ASWTKERWSQ EFTKHQVLIM 150
TCYVALNVLK NGYLSLSDIN LLVFDECHLA ILDHPYREIM KLCESCPSCP 200
RILGLTASIL NGKCDPEELE EKIQKLEKIL KSNAETATDL VVLDRYASQP 250
CEIVVDCGPF TDRSGLYERL LMELEEAINF INDCNVSVHS KERDSTLISK 300
QILSDCRAVL VVLGPWCADK VAGMMVRELQ KYIKHEQEEL HRKFLLFTDT 350
LLRKIHALCE EHFSPASLDL KYVTPKVMKL LEILRKYKPY ERQQFESVEW 400
YNNRNQDNYV SWSDSEDDDD DEEIEEKEKP ETNFPSPFTN ILCGIIFVER 450
RYTAVVLNRL IKEAGKQDPE LAYISSNFIT GHGIGKNQPR SKQMEAEFRK 500
QEEVLRKFRA HETNLLIATS VVEEGVDIPK CNLVVRFDLP TEYRSYVQSK 550
GRARAPISNY VMLADTDKIQ SFEEDLKTYK AIEKILRNKC SKSVDGAEAD 600
VDAVVDDDDV FPPYVLRPDD GGPRVTINTA IGHVNRYCAR LPSDPFTHLA 650
PKCRTQELPD GTFYSTLYLP INSPLRASIV GPPMGCVRLA ERVVALICCE 700
KLHKIGELDE HLMPVGKETV KYEEELDLHD EEETSVPGRP GSTKRRQCYP 750
KAIPECLRES YPKPDQPCYL YVIGMVLTTP LPDELNFRRR KLYPPEDTTR 800
CFGILTAKPI PQIPHFPVYT RSGEVTISIE LKKSGFTLSQ QMLELITRLH 850
QYIFSHILRL EKPALEFKPT GAESAYCVLP LNVVNDSSTL DIDFTFMEDI 900
EKSEARIGIP STKYSKETPF VFKLEDYQDA VIIPRYRNFD QPHRFYVADV 950
YTDLTPLSKF PSPEYETFAE YYKTKYNLDL TNLNQPLLDV DHTSSRLNLL 1000
TPRHLNQKGK ALPLSSAEKR KAKWESLQNK QILVPELCAI HPIPASLWRK 1050
AVCLPSILYR LHCLLTAEEL RAQTASDAGV GVRSLPADFR YPNLDFGWKK 1100
SIDSKSFIST CNSSLAENDN YCKHSTIIVP ENAAHQGATR TPLENHDQMS 1150
VNCRRLPAES SGKLQIEVST DLTAINGLSY NKNLANGSYD LVNRDFCQGN 1200
QLNYFKQEIP VQPTTSYPIQ NLYHYENQPK PSNECTLLSN KYLDGNANTS 1250
TSDGSPAVST MPAVMSAGRA LKDRMDSEQS PSAGYSSRTL GPNPGLILQA 1300
LTLSNASDGF NLERLEMLGD SFLKHAITTY LFCTYPDAHE GRLSYMRSKK 1350
VSNCNLYRLG KKKGLPSRMV VSIFDPPVNW LPPGYVVNQD KSNAEKWEKD 1400
EMTKDCLLAN GKLGKDCEEE EALTWRAPKE EAEYEDDFLE YDQEHIQFID 1450
SMLMGSGAFV KKISLSPFST SDSAYEWKMP KKSSLGSMPF SSDLEDFDYS 1500
SWDAMCYLDP SKAVEEDDFV VGFWNPSEEN CGVDTGKQSI SYDLHTEQCI 1550
ADKSIADCVE ALLGCYLTSC GERAAQLFLC SLGLKVLPVI KRTSREKALY 1600
PAQENFSSQQ KSLSGSCAAT AASPRSSAGK DLEYGCLKIP PRCMFDHPDA 1650
EKTLNHLISG FENFEKKINY RFKNKAYLLQ AFTHASYHYN TITDCYQRLE 1700
FLGDAILDYL ITKHLYEDPR QHSPGVLTDL RSALVNNTIF ASLAVKYDYH 1750
KYFKAVSPEL FHVIDDFVQF QLEKNEMQGM DSELRRSEED EEKEEDIEVP 1800
KAMGDIFESL AGAIYMDSGM SLEVVWQVYY PMMRPLIEKF SANVPRSPVR 1850
ELLEMEPETA KFSPAERTYD GKVRVTVEVV GKGKFKGVGR SYRIAKSAAA 1900
RRALRSLKAN QPQVPNS 1917
Length:1,917
Mass (Da):217,171
Last modified:September 1, 2009 - v3
Checksum:iE42D6F5412E65A0F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF031271 mRNA. Translation: ABK28790.1.
RefSeqiNP_001231198.1. NM_001244269.1.
XP_007647243.1. XM_007649053.1.
XP_007647244.1. XM_007649054.1.

Genome annotation databases

GeneIDi100689239.
KEGGicge:100689239.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EF031271 mRNA. Translation: ABK28790.1 .
RefSeqi NP_001231198.1. NM_001244269.1.
XP_007647243.1. XM_007649053.1.
XP_007647244.1. XM_007649054.1.

3D structure databases

ProteinModelPortali A0MQH0.
SMRi A0MQH0. Positions 1649-1911.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100689239.
KEGGi cge:100689239.

Organism-specific databases

CTDi 23405.

Phylogenomic databases

KOi K11592.

Family and domain databases

Gene3Di 1.10.1520.10. 4 hits.
3.30.160.20. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR005034. Dicer_dimerisation_dom.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014720. dsRNA-bd_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR003100. PAZ_dom.
IPR000999. RNase_III_dom.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF03368. Dicer_dimer. 1 hit.
PF00271. Helicase_C. 1 hit.
PF02170. PAZ. 1 hit.
PF00636. Ribonuclease_3. 2 hits.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00358. DSRM. 1 hit.
SM00490. HELICc. 1 hit.
SM00949. PAZ. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view ]
SUPFAMi SSF101690. SSF101690. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF69065. SSF69065. 4 hits.
PROSITEi PS51327. DICER_DSRBF. 1 hit.
PS50137. DS_RBD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50821. PAZ. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Wallerstorfer D.
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiDICER_CRIGR
AccessioniPrimary (citable) accession number: A0MQH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 1, 2009
Last modified: September 3, 2014
This is version 49 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-11 is the initiator.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi