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Protein

Ethylene-responsive transcription factor ABI4

Gene

ABI4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription regulator that probably binds to the GCC-box pathogenesis-related promoter element. Binds also to the S-box (5'-CACTTCCA-3') photosynthesis-associated nuclear genes-related (PhANGs-related) promoter element, and thus acts as a transcription inhibitor. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways. May have a function in the deetiolation process. Confers sensitivity to abscisic acid (ABA), and regulates the ABA signaling pathway during seed germination, upon nitrate-mediated lateral root inhibition, in hexokinase-dependent sugar responses (including feed-back regulation of photosynthesis and mobilization of storage lipid during germination), and in response to osmotic stress mediated by NaCl, KCl or mannitol. Plays a role in sucrose sensing or signaling, especially at low fluence far red light. Also involved in plant response to glucose treatment, especially at low concentration and in young seedlings. Required for the trehalose-mediated root inhibition and starch accumulation in cotyledons, probably by inhibiting starch breakdown. However, seems to not be involved in sugar-mediated senescence. Required for the ABA-dependent beta-amino-butyric acid (BABA) signaling pathway. BABA primes ABA synthesis and promotes resistance to drought and salt, and leads to a prime callose accumulation that confers resistance against necrotrophic pathogens such as A.brassicicola and P.cucumerina. Seems to be involved in resistance to S.sclerotiorum probably by regulating the ABA-mediated stomatal closure apparently by antagonistic interaction with oxalate. Negative regulator of low water potential-induced Pro accumulation whose effect is decreased by high levels of sugar.23 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi54 – 11158AP2/ERFPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: TAIR
  • transcription factor activity, sequence-specific DNA binding Source: TAIR
  • transcription regulatory region DNA binding Source: TAIR

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: TAIR
  • defense response Source: UniProtKB-KW
  • ethylene-activated signaling pathway Source: UniProtKB-KW
  • lateral root development Source: TAIR
  • mitochondria-nucleus signaling pathway Source: TAIR
  • positive regulation of transcription, DNA-templated Source: TAIR
  • regulation of protein localization Source: TAIR
  • regulation of stomatal movement Source: TAIR
  • regulation of triglyceride catabolic process Source: TAIR
  • response to glucose Source: TAIR
  • response to osmotic stress Source: TAIR
  • response to sucrose Source: TAIR
  • response to trehalose Source: TAIR
  • response to water deprivation Source: TAIR
  • root meristem growth Source: TAIR
  • seed development Source: TAIR
  • starch catabolic process Source: TAIR
  • sugar mediated signaling pathway Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Abscisic acid signaling pathway, Ethylene signaling pathway, Plant defense, Stress response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ethylene-responsive transcription factor ABI4
Short name:
ERF ABI4
Alternative name(s):
Protein ABSCISIC ACID INSENSITIVE 4
Protein GLUCOSE INSENSITIVE 6
Protein IMPAIRED SUCROSE INDUCTION 3
Protein SALOBRENO 5
Protein SUCROSE UNCOUPLED 6
Protein SUGAR INSENSITIVE 5
Gene namesi
Name:ABI4
Synonyms:ERF052, GIN6, ISI3, SAN5, SIS5, SUN6
Ordered Locus Names:At2g40220
ORF Names:T7M7.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G40220.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi69 – 691E → K in abi4-104/sis5-4; impaired ABA signaling pathway in response to sucrose. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Ethylene-responsive transcription factor ABI4PRO_0000297916Add
BLAST

Proteomic databases

PaxDbiA0MES8.
PRIDEiA0MES8.

Expressioni

Tissue specificityi

In seeds, mostly in embryo, and seedlings, especially in vascular tissues. Confined to the hypocotyl, cotyledons, the root cap, and the root quiescent center.4 Publications

Developmental stagei

Levels increase in embryos from globular stage onward during seed maturation. In seedlings, levels in cotyledons and hypocotyls decrease progressively to disappear 3 days after germination, except after glucose treatment that makes levels constant.2 Publications

Inductioni

Only in young seedlings by ABA, imbibition, glucose, 2-deoxy-glucose (2DG), trehalose, and osmotic stress.3 Publications

Gene expression databases

GenevisibleiA0MES8. AT.

Interactioni

Subunit structurei

Interacts with SPK1, SCAR2 and SCAR3.1 Publication

Protein-protein interaction databases

BioGridi3952. 7 interactions.
STRINGi3702.AT2G40220.1.

Structurei

3D structure databases

ProteinModelPortaliA0MES8.
SMRiA0MES8. Positions 55-110.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 AP2/ERF DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410J1R6. Eukaryota.
ENOG410YPH3. LUCA.
HOGENOMiHOG000033770.
InParanoidiA0MES8.
KOiK09286.
OMAiSTSFHHE.
PhylomeDBiA0MES8.

Family and domain databases

Gene3Di3.30.730.10. 1 hit.
InterProiIPR033266. ABI4.
IPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PANTHERiPTHR31241:SF7. PTHR31241:SF7. 1 hit.
PfamiPF00847. AP2. 1 hit.
[Graphical view]
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiSM00380. AP2. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS51032. AP2_ERF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0MES8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPLASQHQH NHLEDNNQTL THNNPQSDST TDSSTSSAQR KRKGKGGPDN
60 70 80 90 100
SKFRYRGVRQ RSWGKWVAEI REPRKRTRKW LGTFATAEDA ARAYDRAAVY
110 120 130 140 150
LYGSRAQLNL TPSSPSSVSS SSSSVSAASS PSTSSSSTQT LRPLLPRPAA
160 170 180 190 200
ATVGGGANFG PYGIPFNNNI FLNGGTSMLC PSYGFFPQQQ QQQNQMVQMG
210 220 230 240 250
QFQHQQYQNL HSNTNNNKIS DIELTDVPVT NSTSFHHEVA LGQEQGGSGC
260 270 280 290 300
NNNSSMEDLN SLAGSVGSSL SITHPPPLVD PVCSMGLDPG YMVGDGSSTI
310 320
WPFGGEEEYS HNWGSIWDFI DPILGEFY
Length:328
Mass (Da):35,671
Last modified:August 21, 2007 - v2
Checksum:iB6B710475E34D08F
GO

Sequence cautioni

The sequence ABK28529.1 differs from that shown. Reason: Erroneous termination at position 329. Translated as stop.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31P → L in AAT44957 (Ref. 3) Curated
Sequence conflicti193 – 1931Missing in AAT44957 (Ref. 3) Curated
Sequence conflicti230 – 2301T → A in AAT44957 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF040959 Genomic DNA. Translation: AAC39489.1.
AF085279 Genomic DNA. Translation: AAD25937.1.
AY560890 mRNA. Translation: AAT44957.1.
CP002685 Genomic DNA. Translation: AEC09798.1.
DQ446612 mRNA. Translation: ABE65896.1.
DQ653050 mRNA. Translation: ABK28529.1. Sequence problems.
PIRiG84826.
RefSeqiNP_181551.1. NM_129580.1.
UniGeneiAt.50594.

Genome annotation databases

EnsemblPlantsiAT2G40220.1; AT2G40220.1; AT2G40220.
GeneIDi818614.
GrameneiAT2G40220.1; AT2G40220.1; AT2G40220.
KEGGiath:AT2G40220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF040959 Genomic DNA. Translation: AAC39489.1.
AF085279 Genomic DNA. Translation: AAD25937.1.
AY560890 mRNA. Translation: AAT44957.1.
CP002685 Genomic DNA. Translation: AEC09798.1.
DQ446612 mRNA. Translation: ABE65896.1.
DQ653050 mRNA. Translation: ABK28529.1. Sequence problems.
PIRiG84826.
RefSeqiNP_181551.1. NM_129580.1.
UniGeneiAt.50594.

3D structure databases

ProteinModelPortaliA0MES8.
SMRiA0MES8. Positions 55-110.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3952. 7 interactions.
STRINGi3702.AT2G40220.1.

Proteomic databases

PaxDbiA0MES8.
PRIDEiA0MES8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G40220.1; AT2G40220.1; AT2G40220.
GeneIDi818614.
GrameneiAT2G40220.1; AT2G40220.1; AT2G40220.
KEGGiath:AT2G40220.

Organism-specific databases

TAIRiAT2G40220.

Phylogenomic databases

eggNOGiENOG410J1R6. Eukaryota.
ENOG410YPH3. LUCA.
HOGENOMiHOG000033770.
InParanoidiA0MES8.
KOiK09286.
OMAiSTSFHHE.
PhylomeDBiA0MES8.

Miscellaneous databases

PROiA0MES8.

Gene expression databases

GenevisibleiA0MES8. AT.

Family and domain databases

Gene3Di3.30.730.10. 1 hit.
InterProiIPR033266. ABI4.
IPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PANTHERiPTHR31241:SF7. PTHR31241:SF7. 1 hit.
PfamiPF00847. AP2. 1 hit.
[Graphical view]
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiSM00380. AP2. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS51032. AP2_ERF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Arabidopsis abscisic acid response locus ABI4 encodes an APETALA 2 domain protein."
    Finkelstein R.R., Wang M.L., Lynch T.J., Rao S., Goodman H.M.
    Plant Cell 10:1043-1054(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "A cluster of ABA-regulated genes on Arabidopsis thaliana BAC T07M07."
    Wang M.L., Belmonte S., Kim U., Dolan M., Morris J.W., Goodman H.M.
    Genome Res. 9:325-333(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Molecular cloning, expression, phylogenetic and functional characterization of the Arabidopsis AP2/EREBP transcription factor family."
    Pan Y., Gong W., Liu D., Fu Q., Mei W.-Q., Song W.-Q., Ma L.-G., Luo J.-C., Deng X.-W., Zhu Y.-X.
    Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations."
    Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.
    Plant Biotechnol. J. 4:317-324(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Sucrose control of phytochrome A signaling in Arabidopsis."
    Dijkwel P.P., Huijser C., Weisbeek P.J., Chua N.-H., Smeekens S.C.M.
    Plant Cell 9:583-595(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "An Arabidopsis mutant showing reduced feedback inhibition of photosynthesis."
    Van Oosten J.-J.M., Gerbaud A., Huijser C., Dijkwel P.P., Chua N.-H., Smeekens S.C.M.
    Plant J. 12:1011-1020(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Genetic analysis of salt-tolerant mutants in Arabidopsis thaliana."
    Quesada V., Ponce M.R., Micol J.L.
    Genetics 154:421-436(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Analysis of Arabidopsis glucose insensitive mutants, gin5 and gin6, reveals a central role of the plant hormone ABA in the regulation of plant vegetative development by sugar."
    Arenas-Huertero F., Arroyo A., Zhou L., Sheen J., Leon P.
    Genes Dev. 14:2085-2096(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Regulation and function of the Arabidopsis ABA-insensitive4 gene in seed and abscisic acid response signaling networks."
    Soederman E.M., Brocard I.M., Lynch T.J., Finkelstein R.R.
    Plant Physiol. 124:1752-1765(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
  12. "The Arabidopsis SUCROSE UNCOUPLED-6 gene is identical to ABSCISIC ACID INSENSITIVE-4: involvement of abscisic acid in sugar responses."
    Huijser C., Kortstee A., Pego J., Weisbeek P.J., Wisman E., Smeekens S.
    Plant J. 23:577-585(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "The Arabidopsis sugar-insensitive mutants sis4 and sis5 are defective in abscisic acid synthesis and response."
    Laby R.J., Kincaid M.S., Kim D., Gibson S.I.
    Plant J. 23:587-596(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLU-69.
  14. "Impaired sucrose-induction mutants reveal the modulation of sugar-induced starch biosynthetic gene expression by abscisic acid signalling."
    Rook F., Corke F., Card R., Munz G., Smith C., Bevan M.W.
    Plant J. 26:421-433(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLU-69.
  15. "ABA plays a central role in mediating the regulatory effects of nitrate on root branching in Arabidopsis."
    Signora L., De Smet I., Foyer C.H., Zhang H.
    Plant J. 28:655-662(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  16. "Plastid redox state and sugars: interactive regulators of nuclear-encoded photosynthetic gene expression."
    Oswald O., Martin T., Dominy P.J., Graham I.A.
    Proc. Natl. Acad. Sci. U.S.A. 98:2047-2052(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  17. "Mobilization of seed storage lipid by Arabidopsis seedlings is retarded in the presence of exogenous sugars."
    To J.P.C., Reiter W.-D., Gibson S.I.
    BMC Plant Biol. 2:4-4(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  18. "A screen for genes that function in abscisic acid signaling in Arabidopsis thaliana."
    Nambara E., Suzuki M., Abrams S., McCarty D.R., Kamiya Y., McCourt P.
    Genetics 161:1247-1255(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  19. "Regulatory networks in seeds integrating developmental, abscisic acid, sugar, and light signaling."
    Brocard-Gifford I.M., Lynch T.J., Finkelstein R.R.
    Plant Physiol. 131:78-92(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  20. "Mechanisms of glucose signaling during germination of Arabidopsis."
    Price J., Li T.-C., Kang S.G., Na J.K., Jang J.-C.
    Plant Physiol. 132:1424-1438(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY IMBIBITION AND GLUCOSE.
  21. "Three genes that affect sugar sensing (abscisic acid insensitive 4, abscisic acid insensitive 5, and constitutive triple response 1) are differentially regulated by glucose in Arabidopsis."
    Arroyo A., Bossi F., Finkelstein R.R., Leon P.
    Plant Physiol. 133:231-242(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
  22. "Interactions of abscisic acid and sugar signalling in the regulation of leaf senescence."
    Pourtau N., Mares M., Purdy S., Quentin N., Rueel A., Wingler A.
    Planta 219:765-772(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  23. "Beta-amino-butyric acid-induced resistance against necrotrophic pathogens is based on ABA-dependent priming for callose."
    Ton J., Mauch-Mani B.
    Plant J. 38:119-130(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  24. "Oxalate production by Sclerotinia sclerotiorum deregulates guard cells during infection."
    Guimaraes R.L., Stotz H.U.
    Plant Physiol. 136:3703-3711(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  25. "Sugar and ABA responsiveness of a minimal RBCS light-responsive unit is mediated by direct binding of ABI4."
    Acevedo-Hernandez G.J., Leon P., Herrera-Estrella L.R.
    Plant J. 43:506-519(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  26. "Enhancing Arabidopsis salt and drought stress tolerance by chemical priming for its abscisic acid responses."
    Jakab G., Ton J., Flors V., Zimmerli L., Metraux J.-P., Mauch-Mani B.
    Plant Physiol. 139:267-274(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  27. "Role of abscisic acid (ABA) and Arabidopsis thaliana ABA-insensitive loci in low water potential-induced ABA and proline accumulation."
    Verslues P.E., Bray E.A.
    J. Exp. Bot. 57:201-212(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  28. "Arabidopsis ABA INSENSITIVE4 regulates lipid mobilization in the embryo and reveals repression of seed germination by the endosperm."
    Penfield S., Li Y., Gilday A.D., Graham S., Graham I.A.
    Plant Cell 18:1887-1899(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  29. "Genome-wide analysis of the ERF gene family in Arabidopsis and rice."
    Nakano T., Suzuki K., Fujimura T., Shinshi H.
    Plant Physiol. 140:411-432(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  30. "The role of Arabidopsis SCAR genes in ARP2-ARP3-dependent cell morphogenesis."
    Uhrig J.F., Mutondo M., Zimmermann I., Deeks M.J., Machesky L.M., Thomas P., Uhrig S., Rambke C., Hussey P.J., Huelskamp M.
    Development 134:967-977(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SPK1; SCAR2 AND SCAR3.
  31. "ABI4 mediates the effects of exogenous trehalose on Arabidopsis growth and starch breakdown."
    Ramon M., Rolland F., Thevelein J.M., van Dijck P., Leyman B.
    Plant Mol. Biol. 63:195-206(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY TREHALOSE.

Entry informationi

Entry nameiABI4_ARATH
AccessioniPrimary (citable) accession number: A0MES8
Secondary accession number(s): O81138, Q6J9N5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 21, 2007
Last sequence update: August 21, 2007
Last modified: May 11, 2016
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

'Salobreno' means 'salty land' in Spanish. Plants lacking ABI4 are salt tolerant.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.