Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A0M5T1 (SYL_GRAFK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:GFO_3032
OrganismGramella forsetii (strain KT0803) [Complete proteome] [HAMAP]
Taxonomic identifier411154 [NCBI]
Taxonomic lineageBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeGramella

Protein attributes

Sequence length962 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 962962Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009347

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif737 – 7415"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7401ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A0M5T1 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: F535EE086A219AC2

FASTA962110,237
        10         20         30         40         50         60 
MSYHFNKIEE KWQKYWADNQ TFKASNDSEK PKYYVLDMFP YPSGAGLHVG HPLGYIASDI 

        70         80         90        100        110        120 
YARFKRHKGF NVLHPQGYDS FGLPAEQYAI QTGQHPAVTT ENNIARYREQ LDKIGFSFDW 

       130        140        150        160        170        180 
SREVRTSEPD YYKWTQWIFI QLFESWYDQE AEKSRAITEL EEIFTSEGNS RINAASDDDV 

       190        200        210        220        230        240 
EKFSADDWNA CSEDEKEQIL LKYRLTYLAE AEVNWCPQLG TVLANDEIVN GVSERGGYPV 

       250        260        270        280        290        300 
VRKKMTQWSM RISAFSERLL QDLNKIDWTE SLKESQRNWI GKSVGAHVDF KIENSKFKIG 

       310        320        330        340        350        360 
VFTTRPDTIF GVSFMTLAPE HELVEKITTE DRKEEVQAYI EASAKRSERE RMADVKTISG 

       370        380        390        400        410        420 
AFTGAYAEHP FTKKPVPIWI GDYVLAGYGT GAVMAVPCGD QRDHDFARHF DLLIPNIFEN 

       430        440        450        460        470        480 
VDVSQEAYAG KEGTVIANSD FLSGLEYKEA LNKVILELEK TGQGYGKTNY RLRDAVFSRQ 

       490        500        510        520        530        540 
RYWGEPFPVY YVNGLPKMID EKYLPLKLPE VEKYLPTETG EPPLGNATDW AWDSKNNKVV 

       550        560        570        580        590        600 
SNKVLKQSRK LSGQHDEPNS NVTPSAVEGS DDNGIYPLEL NTMPGWAGSS WYLFRYMGAG 

       610        620        630        640        650        660 
NPDRFVSEEA QKYWENVDLY IGGSEHATGH LLYSRFWTKF LYDRGWLTVE EPFKKLINQG 

       670        680        690        700        710        720 
MILGTSAFVY RLEGENVFVS KNQINGNNVQ PIHADVSLVN SSDELDIEGF KKWRPEFADA 

       730        740        750        760        770        780 
KFLTEDGKYI VGREVEKMSK SKYNVVNPDE ICQDYGADTL RMYEMFLGPL EQAKPWNTAG 

       790        800        810        820        830        840 
ITGVHNFLKK LWKLYYDGEE FFVSDEKASA DSLKTFHKTI KKVTEDIEEF SFNTSVSTFM 

       850        860        870        880        890        900 
ICVNELTAQK CNSREILEPL AVLIAPYAPH IAEELWEKLG NSESVTTAKY PEFEEKYLVE 

       910        920        930        940        950        960 
SMKNYPVSFN GKMRFTMELS LDMSKEEIEK TVMADERTQK QLDGRTPKKV IVVPGKIVNI 


VG 

« Hide

References

[1]"Whole genome analysis of the marine Bacteroidetes'Gramella forsetii' reveals adaptations to degradation of polymeric organic matter."
Bauer M., Kube M., Teeling H., Richter M., Lombardot T., Allers E., Wuerdemann C.A., Quast C., Kuhl H., Knaust F., Woebken D., Bischof K., Mussmann M., Choudhuri J.V., Meyer F., Reinhardt R., Amann R.I., Gloeckner F.O.
Environ. Microbiol. 8:2201-2213(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KT0803.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU207366 Genomic DNA. Translation: CAL67976.1.
RefSeqYP_863043.1. NC_008571.1.

3D structure databases

ProteinModelPortalA0M5T1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING411154.GFO_3032.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL67976; CAL67976; GFO_3032.
GeneID4652867.
KEGGgfo:GFO_3032.
PATRIC22074808. VBIGraFor5527_2920.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycGFOR411154:GI79-3032-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 5 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_GRAFK
AccessionPrimary (citable) accession number: A0M5T1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 12, 2006
Last modified: April 16, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries