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A0M4U5 (DDL_GRAFK) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:GFO_2684
OrganismGramella forsetii (strain KT0803) [Complete proteome] [HAMAP]
Taxonomic identifier411154 [NCBI]
Taxonomic lineageBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeGramella

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 326326D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341104

Regions

Domain121 – 320200ATP-grasp
Nucleotide binding149 – 20456ATP By similarity

Sites

Metal binding2751Magnesium or manganese 1 By similarity
Metal binding2871Magnesium or manganese 1 By similarity
Metal binding2871Magnesium or manganese 2 By similarity
Metal binding2891Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A0M4U5 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: 15D631E2BC685E20

FASTA32636,436
        10         20         30         40         50         60 
MKKKIAIAMG GYSSEYRISI NSGNIVYKHL DHDLYEPYRV HILKGEWFVV ADDDTPYPIN 

        70         80         90        100        110        120 
KSNFTVKIND KVIQFDCVFN TIHGTPGENG LLQAYLELIG IPQTSCDYYQ SALTFNKRDL 

       130        140        150        160        170        180 
ISVLRPYGIK AATNYFLNKD QEINTKEIVD KVGLPCFVKA NRAGSSFGVT KVKTEDEIIS 

       190        200        210        220        230        240 
AAKTAFTEDD EAIIESFLDG TEVSVGVITY KGKVLALPVT EIIPDGEFFD YEAKYLGKSQ 

       250        260        270        280        290        300 
EITPARISEE DTKKVQDIAI MIYKKLKMKG LSRSEFIFHE GEPHFIEMNT NPGISQASIL 

       310        320 
PQQAEKAGIS LKDLFGSTIE AALNQK 

« Hide

References

[1]"Whole genome analysis of the marine Bacteroidetes'Gramella forsetii' reveals adaptations to degradation of polymeric organic matter."
Bauer M., Kube M., Teeling H., Richter M., Lombardot T., Allers E., Wuerdemann C.A., Quast C., Kuhl H., Knaust F., Woebken D., Bischof K., Mussmann M., Choudhuri J.V., Meyer F., Reinhardt R., Amann R.I., Gloeckner F.O.
Environ. Microbiol. 8:2201-2213(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KT0803.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU207366 Genomic DNA. Translation: CAL67640.1.
RefSeqYP_862707.1. NC_008571.1.

3D structure databases

ProteinModelPortalA0M4U5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING411154.GFO_2684.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL67640; CAL67640; GFO_2684.
GeneID4650906.
KEGGgfo:GFO_2684.
PATRIC22074122. VBIGraFor5527_2589.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAKLAFQYD.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycGFOR411154:GI79-2684-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_GRAFK
AccessionPrimary (citable) accession number: A0M4U5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: December 12, 2006
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways