A0M4S0 (RLMF_GRAFK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase F
EC=2.1.1.181

Alternative name(s):
23S rRNA mA1618 methyltransferase
rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	GFO_2659

Organism

Gramella forsetii (strain KT0803) [Complete proteome] [HAMAP]

Taxonomic identifier

411154 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Gramella

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity. HAMAP MF_01848
Catalytic activity	S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA. HAMAP MF_01848
Subcellular location	Cytoplasm Potential HAMAP MF_01848.
Sequence similarities	Belongs to the methyltransferase superfamily. METTL16/rlmF family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349918

Sequences

	Sequence		Length	Mass (Da)
	A0M4S0 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 90953050DB25D615	FASTA	285	32,511
	10 20 30 40 50 60 MHPDNIHKDS YDFEALTESN PQLSEFVFQN KYGTQTINFA NPEAVLQLNK ALLKFHYQVE 70 80 90 100 110 120 NWSIPKQYLC PPIPGRADYI HYLNDLLSEE NIQGEIRGID IGVGANAIYP ILASRIYDWK 130 140 150 160 170 180 MLGTDIEEKS VAIAQANIET NPTIAKNIEI RHQEDRGSIF KGMIKKGEYY HFSICNPPFH 190 200 210 220 230 240 ASQEEANKAT SRKFKNLGLE KGSALNFGGQ ANELWCNGGE ALFIKRMIKE SVLFKSQVGY 250 260 270 280 FTSLVSKKEN LPKIYKHLNK LKADFKTIEM GQGNKKSRII AWKFD

References

[1]

"Whole genome analysis of the marine Bacteroidetes'Gramella forsetii' reveals adaptations to degradation of polymeric organic matter." Bauer M., Kube M., Teeling H., Richter M., Lombardot T., Allers E., Wuerdemann C.A., Quast C., Kuhl H., Knaust F., Woebken D., Bischof K., Mussmann M., Choudhuri J.V., Meyer F., Reinhardt R., Amann R.I., Gloeckner F.O. Environ. Microbiol. 8:2201-2213(2006) [PubMed: 17107561] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT0803.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU207366 Genomic DNA. Translation: CAL67615.1.
RefSeq	YP_862682.1. NC_008571.1.
3D structure databases
ProteinModelPortal	A0M4S0.
ModBase	Search...
Protein-protein interaction databases
STRING	A0M4S0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4652380.
GenomeReviews	Gene locus GFO_2659 in contig CU207366_GR.
KEGG	gfo:GFO_2659.
NMPDR	fig|411154.5.peg.2510.
PATRIC	22074071. VBIGraFor5527_2564.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3129.
HOGENOM	HBG747880.
OMA	DFANPEA.
PhylomeDB	A0M4S0.
ProtClustDB	PRK11727.
Enzyme and pathway databases
BioCyc	GFOR411154:GFO_2659-MONOMER.
Family and domain databases
HAMAP	MF_01848. 23SrRNA_methyltr_F. [Tree]
InterPro	IPR016909. rRNA_lsu_methyltransferase_F. IPR010286. SAM-Mtase_prd. [Graphical view]
KO	K06970.
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_GRAFK

Accession

Primary (citable) accession number: A0M4S0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program