A0M3Y9 (RPOB_GRAFK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	GFO_2370

Organism

Gramella forsetii (strain KT0803) [Complete proteome] [HAMAP]

Taxonomic identifier

411154 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Gramella

Protein attributes

Sequence length	1270 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1270	1270	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_0000300322

Sequences

	Sequence		Length	Mass (Da)
	A0M3Y9 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: C8F986E1972EA5CF	FASTA	1,270	142,739
	10 20 30 40 50 60 MLAKQTERLS FSSVKNKPAY PDFLDLQIKS FQDFFQLETK SEERGNEGLY NTFLENFPIT 70 80 90 100 110 120 DTRNQFVLEF LDYFVDPPRY SIQECIERGL TYSVPLKARL KLYCTDPEHE DFETIVQDVY 130 140 150 160 170 180 LGTIPYMTPS GTFCINGAER VVVSQLHRSP GVFFGQSFHA NGTKLYSARV IPFKGSWIEF 190 200 210 220 230 240 ATDINSVMYA YIDRKKKLPV TTLFRAIGFE RDKDILEIFD LAEEVKVSKT GLKKYLGRKL 250 260 270 280 290 300 AARVLNTWYE DFVDEDTGEV VSIERNEIVL DRDTELEKDH IEEILETGSK TILLHKEDNQ 310 320 330 340 350 360 TGDYAIIHNT LQKDPTNSEK EAVEHIYRQL RNAEPPDEET ARGIIDKLFF SDQRYSLGEV 370 380 390 400 410 420 GRYRMNKKLG LDVEMDKQVL TKLDIITIVK YLIELINSKA EIDDIDHLSN RRVRTVGEQL 430 440 450 460 470 480 SQQFGVGLAR MARTIRERMN VRDNEVFTPI DLINAKTLSS VINSFFGTNQ LSQFMDQTNP 490 500 510 520 530 540 LAEITHKRRL SALGPGGLSR ERAGFEVRDV HYTHYGRLCP IETPEGPNIG LISSLSVYAK 550 560 570 580 590 600 VNGMGFIETP YRSVTDGKIN TSEEPIYLSA EEEEGKKIAQ ANIPLKDDGT IDTDRVIARM 610 620 630 640 650 660 EGDFPVVDPK EIHYTDVAPN QISSISASLI PFLEHDDANR ALMGSNMMRQ AVPLLRTDSP 670 680 690 700 710 720 IVGTGLERQV ATDSRVLINA EGEGEVEYVD ANKIVIKYDR TEEERMVSFD DDSKSYNLIK 730 740 750 760 770 780 FRKTNQGSCI NLKPIISVGD RVTKGQVLCQ GYATEAGELA LGRNMKVAFM PWKGYNFEDA 790 800 810 820 830 840 IVISEKVVRD DIFTSIHIDE YSLEVRDTKL GNEELTNDIP NVSEEATKDL DEHGMIRVGA 850 860 870 880 890 900 EVKPGDILIG KITPKGESDP TPEEKLLRAI FGDKAGDVKD ASLKASPSLS GVVINKKLFA 910 920 930 940 950 960 RAIKDKRKRA QDKEDVAALE KKYDAKFANL KADLVEKLFT IIGGKTAQGV QNDLGEEVMP 970 980 990 1000 1010 1020 KGKKYTLKML NAVDDYTHLT TGTWTTDDHL NELVADLLHN YKIKENDLQG NLRREKFTVS 1030 1040 1050 1060 1070 1080 VGDELPSGIL KLAKVYIAKK RKLKVGDKMA GRHGNKGIVA RIVRQEDMPF LEDGTPVDIV 1090 1100 1110 1120 1130 1140 LNPLGVPSRM NIGQIYETVL GWAGQKNGKK YATPIFDGAT IEEINDLTDK AGIPRYGHTY 1150 1160 1170 1180 1190 1200 LYDGGTGMRF DQRATVGVIY MLKLGHMIDD KMHARSIGPY SLITQQPLGG KAQFGGQRFG 1210 1220 1230 1240 1250 1260 EMEVWALEAY GASATLREIL TVKSDDVIGR AKTYEAIVKG EPMPEPGLPE SFNVLMHELK 1270 GLGLDIKLEE

References

[1]

"Whole genome analysis of the marine Bacteroidetes'Gramella forsetii' reveals adaptations to degradation of polymeric organic matter." Bauer M., Kube M., Teeling H., Richter M., Lombardot T., Allers E., Wuerdemann C.A., Quast C., Kuhl H., Knaust F., Woebken D., Bischof K., Mussmann M., Choudhuri J.V., Meyer F., Reinhardt R., Amann R.I., Gloeckner F.O. Environ. Microbiol. 8:2201-2213(2006) [PubMed: 17107561] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT0803.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU207366 Genomic DNA. Translation: CAL67334.1.
RefSeq	YP_862401.1. NC_008571.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0M3Y9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4652914.
GenomeReviews	Gene locus GFO_2370 in contig CU207366_GR.
KEGG	gfo:GFO_2370.
NMPDR	fig|411154.5.peg.2250.
PATRIC	22073515. VBIGraFor5527_2294.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0085.
HOGENOM	HBG285547.
OMA	QRDLTYA.
PhylomeDB	A0M3Y9.
ProtClustDB	PRK00405.
Enzyme and pathway databases
BioCyc	GFOR411154:GFO_2370-MONOMER.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 2 hits.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 2 hits. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_GRAFK

Accession

Primary (citable) accession number: A0M3Y9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program