A0M2E8 (KDSB_GRAFK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-deoxy-manno-octulosonate cytidylyltransferase
EC=2.7.7.38

Alternative name(s):
CMP-2-keto-3-deoxyoctulosonic acid synthase
Short name=CKS
Short name=CMP-KDO synthase

Gene names

Name:	kdsB
Ordered Locus Names:	GFO_1823

Organism

Gramella forsetii (strain KT0803) [Complete proteome] [HAMAP]

Taxonomic identifier

411154 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Gramella

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria By similarity. HAMAP MF_00057
Catalytic activity	CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate. HAMAP MF_00057
Pathway	Nucleotide-sugar biosynthesis; CMP-3-deoxy-D-manno-octulosonate biosynthesis; CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP: step 1/1. HAMAP MF_00057
Subcellular location	Cytoplasm Potential HAMAP MF_00057.
Sequence similarities	Belongs to the KdsB family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-manno-octulosonate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	3-deoxy-manno-octulosonate cytidylyltransferase HAMAP MF_00057		PRO_0000370075

Sequences

	Sequence		Length	Mass (Da)
	A0M2E8 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: F4EA7F868673B76E	FASTA	248	28,245
	10 20 30 40 50 60 MKEPSKNRIV AMIPARYKAS RFPGKLMKDL NGKTVIARTY EAAVNTELFD EVYVVTDSNK 70 80 90 100 110 120 IFDEIVNEGG QVIRSKKEHE CGSDRIAEAV ENMDVDIVVN VQGDEPFIDK NSLAKLLKVF 130 140 150 160 170 180 EQEGAEEIDL TSLKTPLKDS DDITNPNNVK VITGKDDFAL YFSRFPIPYR RDTSANVTYY 190 200 210 220 230 240 KHIGIYAFRK SALMDFYRLP MLHLEAAEKI ECIRYLEYGK KIKMVETSVK SVGIDTPEDL EKARKLLS

References

[1]

"Whole genome analysis of the marine Bacteroidetes'Gramella forsetii' reveals adaptations to degradation of polymeric organic matter." Bauer M., Kube M., Teeling H., Richter M., Lombardot T., Allers E., Wuerdemann C.A., Quast C., Kuhl H., Knaust F., Woebken D., Bischof K., Mussmann M., Choudhuri J.V., Meyer F., Reinhardt R., Amann R.I., Gloeckner F.O. Environ. Microbiol. 8:2201-2213(2006) [PubMed: 17107561] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT0803.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU207366 Genomic DNA. Translation: CAL66793.1.
RefSeq	YP_861860.1. NC_008571.1.
3D structure databases
ProteinModelPortal	A0M2E8.
ModBase	Search...
Protein-protein interaction databases
STRING	A0M2E8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4652783.
GenomeReviews	Gene locus GFO_1823 in contig CU207366_GR.
KEGG	gfo:GFO_1823.
NMPDR	fig|411154.5.peg.1727.
PATRIC	22072429. VBIGraFor5527_1757.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1212.
HOGENOM	HBG637773.
OMA	INVQADE.
PhylomeDB	A0M2E8.
ProtClustDB	CLSK851559.
Enzyme and pathway databases
BioCyc	GFOR411154:GFO_1823-MONOMER.
Family and domain databases
HAMAP	MF_00057. CMP-KDO_synth. [Tree]
InterPro	IPR003329. Cytidylyl_trans. IPR004528. KdsB. [Graphical view]
KO	K00979.
PANTHER	PTHR21485:SF4. PTHR21485:SF4. 1 hit.
Pfam	PF02348. CTP_transf_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00466. KdsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSB_GRAFK

Accession

Primary (citable) accession number: A0M2E8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program