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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Gramella forsetii (strain KT0803)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei188SubstrateUniRule annotation1
Active sitei189Proton donor/acceptorUniRule annotation1
Active sitei319UniRule annotation1
Binding sitei320SubstrateUniRule annotation1
Sitei332Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotationImported
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotationImported
Ordered Locus Names:GFO_1808Imported
OrganismiGramella forsetii (strain KT0803)Imported
Taxonomic identifieri411154 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeGramella
Proteomesi
  • UP000000755 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi411154.GFO_1808.

Structurei

3D structure databases

ProteinModelPortaliA0M2D3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 343Lyase_1InterPro annotationAdd BLAST333
Domaini409 – 462FumaraseC_CInterPro annotationAdd BLAST54

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni98 – 100Substrate bindingUniRule annotation3
Regioni140 – 142Substrate bindingUniRule annotation3
Regioni325 – 327Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q. Bacteria.
COG0114. LUCA.
HOGENOMiHOG000061736.
KOiK01679.
OMAiVSFTDNC.
OrthoDBiPOG091H01XG.

Family and domain databases

CDDicd01362. Fumarase_classII. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

A0M2D3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYRIEKDTM GEVKVPSDKL WGAQTERSRN NFKIGPASSM PLDIIYGFAY
60 70 80 90 100
LKKAAAYTNC ELGVLPVDKR DLVAQVCDEI LEGKHDDQFP LVIWQTGSGT
110 120 130 140 150
QSNMNVNEVI ANRAHQLAGK TIGEGDKTLQ PNDDVNKSQS SNDTFPTGMH
160 170 180 190 200
IAAYNKITKV TLPGLRKLRD TLKKKSEEFK NTVKIGRTHF MDATPLTLGQ
210 220 230 240 250
EFSGYVAQLD YGIKALENTL PHLAELALGG TAVGTGLNTP KGYSKRVAEF
260 270 280 290 300
IAKFTDLPFV SAANKFEALA AHDAFVETHG ALKQLAVSLN KIGNDIRMLA
310 320 330 340 350
SGPRSGIGEI NIPANEPGSS IMPGKVNPTQ CEALTMVCAQ VIGNDVTMSV
360 370 380 390 400
GGMQGQFELN VFKPVMANAL LQSAQLLGDA CDSFEEHCAR GIEANEKRIQ
410 420 430 440 450
EHLNNSLMLV TALNTKIGYY KAAEIANTAH KNGTTLKQEA ANLGYVTEEQ
460
FDDWVDPKDM VGNTK
Length:465
Mass (Da):50,556
Last modified:December 12, 2006 - v1
Checksum:iF33CC1AF437D9A49
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU207366 Genomic DNA. Translation: CAL66778.1.
RefSeqiWP_011709686.1. NC_008571.1.

Genome annotation databases

EnsemblBacteriaiCAL66778; CAL66778; GFO_1808.
KEGGigfo:GFO_1808.

Similar proteinsi

Entry informationi

Entry nameiA0M2D3_GRAFK
AccessioniPrimary (citable) accession number: A0M2D3
Entry historyiIntegrated into UniProtKB/TrEMBL: December 12, 2006
Last sequence update: December 12, 2006
Last modified: September 27, 2017
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported