A0M0C9 (NTPA_GRAFK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

GFO_1100

Organism

Gramella forsetii (strain KT0803) [Complete proteome] [HAMAP]

Taxonomic identifier

411154 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Gramella

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 190	190	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_1000068423
Regions
	Region	7 – 12	6	Substrate binding By similarity
	Region	68 – 69	2	Substrate binding By similarity
Sites
	Metal binding	39	1	Magnesium or manganese By similarity
	Metal binding	68	1	Magnesium or manganese By similarity
	Binding site	151	1	Substrate By similarity
	Binding site	171	1	Substrate By similarity
	Binding site	177	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0M0C9 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: E6CD7E2C7B89DE1C	FASTA	190	21,478
	10 20 30 40 50 60 MKLVFATHNP NKFREIKSLV PKHIELLSLS DINCNEDIEE TGDTIDENAM IKADYVRNHY 70 80 90 100 110 120 GYDCFADDTG LEVHSLAGAP GVYSARYAGD EKNDEANIEK LLEQLKKRDD RTARFKTVIA 130 140 150 160 170 180 LNLKGNQNLF TGICEGEILE EKTGTKGFGY DPIFLPNGFE SSFAEMELTE KSKISHRGIA 190 FRELMEYLSK

References

[1]

"Whole genome analysis of the marine Bacteroidetes'Gramella forsetii' reveals adaptations to degradation of polymeric organic matter." Bauer M., Kube M., Teeling H., Richter M., Lombardot T., Allers E., Wuerdemann C.A., Quast C., Kuhl H., Knaust F., Woebken D., Bischof K., Mussmann M., Choudhuri J.V., Meyer F., Reinhardt R., Amann R.I., Gloeckner F.O. Environ. Microbiol. 8:2201-2213(2006) [PubMed: 17107561] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT0803.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU207366 Genomic DNA. Translation: CAL66074.1.
RefSeq	YP_861141.1. NC_008571.1.
3D structure databases
ProteinModelPortal	A0M0C9.
ModBase	Search...
Protein-protein interaction databases
STRING	A0M0C9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4649512.
GenomeReviews	Gene locus GFO_1100 in contig CU207366_GR.
KEGG	gfo:GFO_1100.
NMPDR	fig|411154.5.peg.1042.
PATRIC	22071027. VBIGraFor5527_1060.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0127.
HOGENOM	HBG697237.
OMA	GVYTADW.
PhylomeDB	A0M0C9.
ProtClustDB	PRK14823.
Enzyme and pathway databases
BioCyc	GFOR411154:GFO_1100-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_GRAFK

Accession

Primary (citable) accession number: A0M0C9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program