Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Gramella forsetii (strain KT0803)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotationSAAS annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi159Zinc 2UniRule annotation1
Metal bindingi162Zinc 2UniRule annotation1
Metal bindingi185Zinc 2UniRule annotation1
Metal bindingi188Zinc 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperoneUniRule annotationSAAS annotation
Biological processDNA replicationUniRule annotationSAAS annotation, Stress responseUniRule annotationSAAS annotation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotationImported
Ordered Locus Names:GFO_0505Imported
OrganismiGramella forsetii (strain KT0803)Imported
Taxonomic identifieri411154 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeGramella
Proteomesi
  • UP000000755 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi411154.GFO_0505.

Structurei

3D structure databases

ProteinModelPortaliA0LYP3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 69JInterPro annotationAdd BLAST66

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotationSAAS annotation

Keywords - Domaini

RepeatUniRule annotationSAAS annotation, Zinc-fingerSAAS annotation

Phylogenomic databases

eggNOGiENOG4105BZ5. Bacteria.
COG0484. LUCA.
HOGENOMiHOG000226717.
KOiK03686.
OMAiDMGGFAD.
OrthoDBiPOG091H00PT.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
cd10719. DnaJ_zf. 1 hit.
Gene3Di1.10.287.110. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiView protein in InterPro
IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR036869. DnaJ_dom_sf.
IPR001623. DnaJ_domain.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
IPR036410. HSP_DnaJ_Cys-rich_dom_sf.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiView protein in PROSITE
PS50076. DNAJ_2. 1 hit.

Sequencei

Sequence statusi: Complete.

A0LYP3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEDYYEILG LSKDASATEI KKAYRKKALK YHPDKNPGDS GAEDMFKKSA
60 70 80 90 100
EAYEVLGNQE KRAKYDRFGH QAFDGGGFGG GGGMNMDDIF SQFGDIFGGG
110 120 130 140 150
FGGGGFSGFG GFGGGQRRAK GSNLRIRVSL TLEEIANGCE KKIKVKRKIQ
160 170 180 190 200
AAGTSYKTCS TCNGTGQVTR VTNTILGRMQ TASPCTTCGG SGQMIDKKPD
210 220 230 240 250
GADAQGLEMK EETVSIKIPP GVEDGMQLKV SGKGNDAPGN GIPGDLLVAI
260 270 280 290 300
EEKEHASLQR EGDNLHYDLY ISYSEAALGT SKEVDTVTGK VRIKIEEGVQ
310 320 330 340 350
SGKILRLRGK GISSINGYGK GDLLVHVNVW TPKTLSREQK EFFERMADDE
360 370
NFQPNPEKSD KSFFEKVKDM FS
Length:372
Mass (Da):40,244
Last modified:December 12, 2006 - v1
Checksum:i4D54FC5C55A30A63
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU207366 Genomic DNA. Translation: CAL65488.1.
RefSeqiWP_011708426.1. NC_008571.1.

Genome annotation databases

EnsemblBacteriaiCAL65488; CAL65488; GFO_0505.
KEGGigfo:GFO_0505.

Similar proteinsi

Entry informationi

Entry nameiA0LYP3_GRAFK
AccessioniPrimary (citable) accession number: A0LYP3
Entry historyiIntegrated into UniProtKB/TrEMBL: December 12, 2006
Last sequence update: December 12, 2006
Last modified: October 25, 2017
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported