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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Gramella forsetii (strain KT0803)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotationSAAS annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi159 – 1591Zinc 2UniRule annotation
Metal bindingi162 – 1621Zinc 2UniRule annotation
Metal bindingi185 – 1851Zinc 2UniRule annotation
Metal bindingi188 – 1881Zinc 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri123 – 21189CR-typeUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

ChaperoneUniRule annotationSAAS annotation

Keywords - Biological processi

DNA replicationUniRule annotationSAAS annotation, Stress responseUniRule annotationSAAS annotation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciGFOR411154:GI79-505-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotationImported
Ordered Locus Names:GFO_0505Imported
OrganismiGramella forsetii (strain KT0803)Imported
Taxonomic identifieri411154 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeGramella
Proteomesi
  • UP000000755 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi411154.GFO_0505.

Structurei

3D structure databases

ProteinModelPortaliA0LYP3.
SMRiA0LYP3. Positions 2-77.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 6966JInterPro annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi76 – 12146Gly-richUniRule annotationAdd
BLAST

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotationSAAS annotation
Contains 1 CR-type zinc finger.UniRule annotation
Contains 1 J domain.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri123 – 21189CR-typeUniRule annotationAdd
BLAST

Keywords - Domaini

RepeatUniRule annotationSAAS annotation, Zinc-fingerUniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105BZ5. Bacteria.
COG0484. LUCA.
HOGENOMiHOG000226717.
KOiK03686.
OMAiYDQIRAM.
OrthoDBiEOG6BPDKP.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiPS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0LYP3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEDYYEILG LSKDASATEI KKAYRKKALK YHPDKNPGDS GAEDMFKKSA
60 70 80 90 100
EAYEVLGNQE KRAKYDRFGH QAFDGGGFGG GGGMNMDDIF SQFGDIFGGG
110 120 130 140 150
FGGGGFSGFG GFGGGQRRAK GSNLRIRVSL TLEEIANGCE KKIKVKRKIQ
160 170 180 190 200
AAGTSYKTCS TCNGTGQVTR VTNTILGRMQ TASPCTTCGG SGQMIDKKPD
210 220 230 240 250
GADAQGLEMK EETVSIKIPP GVEDGMQLKV SGKGNDAPGN GIPGDLLVAI
260 270 280 290 300
EEKEHASLQR EGDNLHYDLY ISYSEAALGT SKEVDTVTGK VRIKIEEGVQ
310 320 330 340 350
SGKILRLRGK GISSINGYGK GDLLVHVNVW TPKTLSREQK EFFERMADDE
360 370
NFQPNPEKSD KSFFEKVKDM FS
Length:372
Mass (Da):40,244
Last modified:December 12, 2006 - v1
Checksum:i4D54FC5C55A30A63
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU207366 Genomic DNA. Translation: CAL65488.1.
RefSeqiWP_011708426.1. NC_008571.1.

Genome annotation databases

EnsemblBacteriaiCAL65488; CAL65488; GFO_0505.
KEGGigfo:GFO_0505.
PATRICi22069865. VBIGraFor5527_0488.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU207366 Genomic DNA. Translation: CAL65488.1.
RefSeqiWP_011708426.1. NC_008571.1.

3D structure databases

ProteinModelPortaliA0LYP3.
SMRiA0LYP3. Positions 2-77.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi411154.GFO_0505.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL65488; CAL65488; GFO_0505.
KEGGigfo:GFO_0505.
PATRICi22069865. VBIGraFor5527_0488.

Phylogenomic databases

eggNOGiENOG4105BZ5. Bacteria.
COG0484. LUCA.
HOGENOMiHOG000226717.
KOiK03686.
OMAiYDQIRAM.
OrthoDBiEOG6BPDKP.

Enzyme and pathway databases

BioCyciGFOR411154:GI79-505-MONOMER.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiPS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Whole genome analysis of the marine Bacteroidetes'Gramella forsetii' reveals adaptations to degradation of polymeric organic matter."
    Bauer M., Kube M., Teeling H., Richter M., Lombardot T., Allers E., Wuerdemann C.A., Quast C., Kuhl H., Knaust F., Woebken D., Bischof K., Mussmann M., Choudhuri J.V., Meyer F., Reinhardt R., Amann R.I., Gloeckner F.O.
    Environ. Microbiol. 8:2201-2213(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KT0803Imported.

Entry informationi

Entry nameiA0LYP3_GRAFK
AccessioniPrimary (citable) accession number: A0LYP3
Entry historyi
Integrated into UniProtKB/TrEMBL: December 12, 2006
Last sequence update: December 12, 2006
Last modified: June 8, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.