A0LYF8 (A0LYF8_GRAFK) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit 2 HAMAP MF_00444
EC=3.4.21.92 HAMAP MF_00444

Alternative name(s):
Endopeptidase Clp 2 HAMAP MF_00444

Gene names

Name:	clpP EMBL CAL65403.1
Synonyms:	clpP2 HAMAP MF_00444
Ordered Locus Names:	GFO_0418

Organism

Gramella forsetii (strain KT0803) [Complete proteome] [HAMAP]

Taxonomic identifier

411154 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Gramella

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family. RuleBase RU003567 HAMAP MF_00444

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP MF_00444
Ligand	ATP-binding HAMAP MF_00444 Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease HAMAP MF_00444
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	92	1	By similarity HAMAP MF_00444
	Active site	117	1	By similarity HAMAP MF_00444

Sequences

	Sequence		Length	Mass (Da)
	A0LYF8 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: B89D611F3906FC75	FASTA	186	20,672
	10 20 30 40 50 60 MDLMKNKTGK IQDLIDNKLL EKRKIYMWGE VSDKTAKYVI DRLEYLDLEG DGEIQLFINS 70 80 90 100 110 120 PGGYVTDGFA IYDTIMSLES PVSTICTGLA ASMGSILLSA GKKGRRFIQP HAKVMIHQPS 130 140 150 160 170 180 GGARGQASNI EIAANEILKT KHLSAEIIAE NCGQSVDKVL KDFNRDYWMD AEESMEYGIV DGIFKK

References

[1]

"Whole genome analysis of the marine Bacteroidetes'Gramella forsetii' reveals adaptations to degradation of polymeric organic matter." Bauer M., Kube M., Teeling H., Richter M., Lombardot T., Allers E., Wuerdemann C.A., Quast C., Kuhl H., Knaust F., Woebken D., Bischof K., Mussmann M., Choudhuri J.V., Meyer F., Reinhardt R., Amann R.I., Gloeckner F.O. Environ. Microbiol. 8:2201-2213(2006) [PubMed: 17107561] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU207366 Genomic DNA. Translation: CAL65403.1.
RefSeq	YP_860470.1. NC_008571.1.
3D structure databases
ProteinModelPortal	A0LYF8.
ModBase	Search...
Protein-protein interaction databases
STRING	A0LYF8.
Protein family/group databases
MEROPS	S14.005.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4651669.
GenomeReviews	Gene locus GFO_0418 in contig CU207366_GR.
KEGG	gfo:GFO_0418.
NMPDR	fig|411154.5.peg.395.
PATRIC	22069703. VBIGraFor5527_0409.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0740.
HOGENOM	HBG558421.
OMA	FSEPVTD.
ProtClustDB	CLSK2533504.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR023562. Pept_S14/S49. IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_AS. [Graphical view]
KO	K01358.
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A0LYF8_GRAFK

Accession

Primary (citable) accession number: A0LYF8

Entry history

Integrated into UniProtKB/TrEMBL:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)