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Protein

D-aminoacyl-tRNA deacylase

Gene

dtd

Organism
Gramella forsetii (strain KT0803)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.

Catalytic activityi

Glycyl-tRNA(Ala) + H2O = glycine + tRNA(Ala).
A D-aminoacyl-tRNA + H2O = a D-amino acid + tRNA.

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
D-aminoacyl-tRNA deacylase (EC:3.1.1.96)
Short name:
DTD
Alternative name(s):
Gly-tRNA(Ala) deacylase (EC:3.1.1.-)
Gene namesi
Name:dtd
Ordered Locus Names:GFO_0346
OrganismiGramella forsetii (strain KT0803)
Taxonomic identifieri411154 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeGramella
Proteomesi
  • UP000000755 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000508351 – 150D-aminoacyl-tRNA deacylaseAdd BLAST150

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi411154.GFO_0346.

Structurei

3D structure databases

ProteinModelPortaliA0LY87.
SMRiA0LY87.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi138 – 139Gly-cisPro motif, important for rejection of L-amino acids2

Domaini

A Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids.

Sequence similaritiesi

Belongs to the DTD family.

Phylogenomic databases

eggNOGiENOG4108YYA. Bacteria.
COG1490. LUCA.
HOGENOMiHOG000113982.
KOiK07560.
OMAiDGPVTIW.
OrthoDBiPOG091H02D0.

Family and domain databases

Gene3Di3.50.80.10. 1 hit.
HAMAPiMF_00518. Deacylase_Dtd. 1 hit.
InterProiView protein in InterPro
IPR003732. Daa-tRNA_deacyls_DTD.
IPR023509. DTD-like_dom_sf.
PANTHERiPTHR10472. PTHR10472. 1 hit.
PfamiView protein in Pfam
PF02580. Tyr_Deacylase. 1 hit.
SUPFAMiSSF69500. SSF69500. 1 hit.
TIGRFAMsiTIGR00256. TIGR00256. 1 hit.

Sequencei

Sequence statusi: Complete.

A0LY87-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAVIQRVSE ASVTVDHKVC AVMRDGLLIL LGIENEDNEE DIDWLCRKII
60 70 80 90 100
NMRIFNDEDE VMNESLKSVD GDAIIVSQFT LHASTKKGNR PSYIKAAKPE
110 120 130 140 150
VAEPLYLKFI SKFQNELGKD VGSGIFGGDM KVSLLNDGPV TIIIDSKEKR
Length:150
Mass (Da):16,739
Last modified:December 12, 2006 - v1
Checksum:i747A190FCE0D56C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU207366 Genomic DNA. Translation: CAL65332.1.
RefSeqiWP_011708270.1. NC_008571.1.

Genome annotation databases

EnsemblBacteriaiCAL65332; CAL65332; GFO_0346.
KEGGigfo:GFO_0346.

Similar proteinsi

Entry informationi

Entry nameiDTD_GRAFK
AccessioniPrimary (citable) accession number: A0LY87
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 12, 2006
Last modified: November 22, 2017
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families