A0LY29 (TYSY_GRAFK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	GFO_0288

Organism

Gramella forsetii (strain KT0803) [Complete proteome] [HAMAP]

Taxonomic identifier

411154 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Gramella

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	Thymidylate synthase HAMAP MF_00008		PRO_1000000604
Sites
	Active site	156	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0LY29 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 5FA4DF324CE1001A	FASTA	274	31,440
	10 20 30 40 50 60 MKQYHDLLKH VLEQGAQKGD RTGTGTKSVF GYQMRFDLSE GFPMVTTKKL HLKSIIYELL 70 80 90 100 110 120 WFLKGDTNIE YLKENGVRIW NEWADDNGDL GPVYGHQWRN WNGEEIDQIK EIVHSLKHNP 130 140 150 160 170 180 NSRRMLVSAW NPSVLPDTSV SFSENVANGK AALPPCHAFF QFYVADGKLS CQLYQRSADI 190 200 210 220 230 240 FLGVPFNIAS YALLTMMMAQ VCGYEAGDFI HTFGDAHIYS NHMEQVELQL SREPRQLPIM 250 260 270 KINPEVKDIF DFKFEDFTLE DYDPHPGIKA KVAI

References

[1]

"Whole genome analysis of the marine Bacteroidetes'Gramella forsetii' reveals adaptations to degradation of polymeric organic matter." Bauer M., Kube M., Teeling H., Richter M., Lombardot T., Allers E., Wuerdemann C.A., Quast C., Kuhl H., Knaust F., Woebken D., Bischof K., Mussmann M., Choudhuri J.V., Meyer F., Reinhardt R., Amann R.I., Gloeckner F.O. Environ. Microbiol. 8:2201-2213(2006) [PubMed: 17107561] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT0803.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU207366 Genomic DNA. Translation: CAL65274.1.
RefSeq	YP_956876.1. NC_008571.1.
3D structure databases
ProteinModelPortal	A0LY29.
SMR	A0LY29. Positions 1-274.
ModBase	Search...
Protein-protein interaction databases
STRING	A0LY29.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4652998.
GenomeReviews	Gene locus GFO_0288 in contig CU207366_GR.
KEGG	gfo:GFO_0288.
NMPDR	fig|411154.5.peg.271.
PATRIC	22069447. VBIGraFor5527_0282.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
HOGENOM	HBG588098.
OMA	RMALAPC.
PhylomeDB	A0LY29.
ProtClustDB	PRK01827.
Enzyme and pathway databases
BioCyc	GFOR411154:GFO_0288-MONOMER.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KO	K00560.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. Thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_GRAFK

Accession

Primary (citable) accession number: A0LY29

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program