Reviewed, UniProtKB/Swiss-Prot A0LV70 (TRMD_ACIC1)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	Acel_1558

Organism

Acidothermus cellulolyticus (strain ATCC 43068 / 11B) [Complete proteome] [HAMAP]

Taxonomic identifier

351607 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Acidothermaceae › Acidothermus

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_1000006442
Regions
	Region	140 – 145	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	116	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0LV70-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: E0521B196FC8189E	FASTA	286	30,687
	10 20 30 40 50 60 MHVDIVSIFP EYFRPLELSL IGKARARGVL DVHLWDLRDF THDPHRTVDD TPYGGGPGMV 70 80 90 100 110 120 MRPEPWGETL DAVRAEAARL GVDAPLLIVP TPAGELLTQR AAERYAAEPW LAFACGRYEG 130 140 150 160 170 180 IDERVLLDAR RHMRVEEVSI GDYVLAGGEA ATLVIVEAVA RLLPGVVGNQ ASVLDDSHAQ 190 200 210 220 230 240 GLLEGPAYTK PAVWRGLEVP EVLLSGNHAA IARWRREQAI RRTAVRRPEL LDALPPGSLT 250 260 270 280 PHEEALAAEA RLHAGRSAET PPPAGAAGSQ AEGPPGTSPS DAAVAH

References

[1]

"Complete sequence of Acidothermus cellulolyticus 11B." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Zharchuk I., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Berry A.M., Adney W.S., Normand P., Leu D., Pujic P., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000481 Genomic DNA. Translation: ABK53330.1.
RefSeq	YP_873316.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0LV70.
Genome annotation databases
GeneID	4485354.
GenomeReviews	Gene locus Acel_1558 in contig CP000481_GR.
KEGG	ace:Acel_1558.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HRSVDDT.
Family and domain databases
HAMAP	MF_00605. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProDom	PD004978. tRNA_m1G_mtfrase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_ACIC1

Accession

Primary (citable) accession number: A0LV70

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)