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Protein

Proline--tRNA ligase

Gene

proS

Organism
Acidothermus cellulolyticus (strain ATCC 43068 / 11B)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.UniRule annotation

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciACEL351607:G1G7K-1555-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
Alternative name(s):
Prolyl-tRNA synthetaseUniRule annotation
Short name:
ProRSUniRule annotation
Gene namesi
Name:proSUniRule annotation
Ordered Locus Names:Acel_1519
OrganismiAcidothermus cellulolyticus (strain ATCC 43068 / 11B)
Taxonomic identifieri351607 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaAcidothermalesAcidothermaceaeAcidothermus
Proteomesi
  • UP000008221 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002883031 – 583Proline--tRNA ligaseAdd BLAST583

Proteomic databases

PRIDEiA0LV31

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi351607.Acel_1519

Structurei

3D structure databases

ProteinModelPortaliA0LV31
SMRiA0LV31
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C90 Bacteria
COG0442 LUCA
HOGENOMiHOG000076893
KOiK01881
OMAiIQPAELW
OrthoDBiPOG091H0290

Family and domain databases

CDDicd00779 ProRS_core_prok, 1 hit
Gene3Di3.40.50.800, 2 hits
3.90.960.10, 1 hit
HAMAPiMF_01569 Pro_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR006195 aa-tRNA-synth_II
IPR004154 Anticodon-bd
IPR036621 Anticodon-bd_dom_sf
IPR002316 Pro-tRNA-ligase_IIa
IPR004500 Pro-tRNA-synth_IIa_bac-type
IPR023717 Pro-tRNA-Synthase_IIa_type1
IPR033730 ProRS_core_prok
IPR036754 YbaK/aa-tRNA-synt-asso_dom_sf
IPR007214 YbaK/aa-tRNA-synth-assoc-dom
PfamiView protein in Pfam
PF03129 HGTP_anticodon, 1 hit
PF00587 tRNA-synt_2b, 1 hit
PF04073 tRNA_edit, 1 hit
PRINTSiPR01046 TRNASYNTHPRO
SUPFAMiSSF55826 SSF55826, 1 hit
TIGRFAMsiTIGR00409 proS_fam_II, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

A0LV31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQRMSKLFV RTLREDPADA EVPSHRLLVR AGYIRRVAPG IYSWLPLGYA
60 70 80 90 100
VLRKIEEIVR QEMLHIGGQE VHFPALIPRE IYEASGRWNE YGDTLFRLKD
110 120 130 140 150
RKGADYLLGP THEELFTLLV KGEYSSYKDY PVILFQIQTK YRDEARPRAG
160 170 180 190 200
ILRGREFVMK DSYSFDLDDA GLAASYTAHR EAYRRIFDRL GLRYRIVSAV
210 220 230 240 250
SGAMGGSASE EFLAVTEVGE DAFVYCRNCD YAANTEAVEI GVPDRLDPTG
260 270 280 290 300
QPPPQVLDTP DTPTIDALVD RLNSLDLGRR FTAADTLKNV VLKTKAPGAD
310 320 330 340 350
DWELLVVGVP GDRDVDLKRL AGQLDPIQVE PATAEDLARR PELVRGYIGP
360 370 380 390 400
QILRRIGVRY LVDPLVVPGS AWVTGANEPD KHMANVVRGR DFEPDGEIGA
410 420 430 440 450
ATIRDGDPCG RCGGRLALAQ GIEIGHIFQL GRKYTDAFEL DALGPDGRPI
460 470 480 490 500
RITMGSYGIG VSRAVAAIAE QCHDDAGLIW PRSVSPADVH VVIAGKGEQE
510 520 530 540 550
KLAEEFAAAL DTAGLDVLLD DRVDASAGVK FADAELIGIP SIAVVGRGAA
560 570 580
RGVVEVRDRG SGERRELALT DAVAELRTLA AGA
Length:583
Mass (Da):63,735
Last modified:December 12, 2006 - v1
Checksum:iB81ADC793825978B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000481 Genomic DNA Translation: ABK53291.1
RefSeqiWP_011720354.1, NC_008578.1

Genome annotation databases

EnsemblBacteriaiABK53291; ABK53291; Acel_1519
KEGGiace:Acel_1519

Similar proteinsi

Entry informationi

Entry nameiSYP_ACIC1
AccessioniPrimary (citable) accession number: A0LV31
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: December 12, 2006
Last modified: May 23, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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