Reviewed, UniProtKB/Swiss-Prot A0LV15 (DAPA_ACIC1)

Names and origin

Protein names

Recommended name:
    Dihydrodipicolinate synthase
      Short name=DHDPS
    EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	Acel_1503

Organism

Acidothermus cellulolyticus (strain ATCC 43068 / 11B) [Complete proteome] [HAMAP]

Taxonomic identifier

351607 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Acidothermaceae › Acidothermus

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_0000340930
Regions
	Region	56 – 57	2	Pyruvate binding By similarity
Sites
	Active site	169	1	Schiff-base intermediate with substrate By similarity
	Binding site	114	1	Pyruvate By similarity
	Site	141	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0LV15-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 8616F8A6633E9D34	FASTA	308	32,423
	10 20 30 40 50 60 MTTSLRPAPF GRVLTAMVTP FTADGALDLD GAARLATYLV DHGNDGLVIS GTTGESPTTT 70 80 90 100 110 120 DDEKERLLRA VLDAVGDRAT VVAGVGTNDT RHTIELAQRA EKAGAHGLLV VTPYYSKPPQ 130 140 150 160 170 180 AGLLAHFRQV ADATGLPVML YDIPGRTGTA IEPETMVRLA EHERIVAVKD AKGDFEASSW 190 200 210 220 230 240 VLARTDLAYY SGDDKNTLPL LAIGAVGVVG VPTHVFGTQT GAMIAAYLRG DVDGALALHR 250 260 270 280 290 300 QLLPVFTGFF RTQGVILAKA ALRLAGLPGG PVRPPLVDAT AEQVARLRED MAAAGFTEFA EGAEERRG

References

[1]

"Complete sequence of Acidothermus cellulolyticus 11B." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Zharchuk I., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Berry A.M., Adney W.S., Normand P., Leu D., Pujic P., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000481 Genomic DNA. Translation: ABK53275.1.
RefSeq	YP_873261.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0LV15.
Genome annotation databases
GeneID	4486267.
GenomeReviews	Gene locus Acel_1503 in contig CP000481_GR.
KEGG	ace:Acel_1503.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CISAVSN.
Family and domain databases
HAMAP	MF_00418. [Tree]
InterPro	IPR005263. DapA_synth. IPR002220. DHDPS. [Graphical view]
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PRINTS	PR00146. DHPICSNTHASE.
ProDom	PD001859. DHDPS. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00674. dapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_ACIC1

Accession

Primary (citable) accession number: A0LV15

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)