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Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Acidothermus cellulolyticus (strain ATCC 43068 / 11B)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Diaminopimelate epimerase (dapF)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14SubstrateUniRule annotation1
Binding sitei97SubstrateUniRule annotation1
Active sitei106Proton donorUniRule annotation1
Binding sitei178SubstrateUniRule annotation1
Sitei180Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Binding sitei209SubstrateUniRule annotation1
Sitei227Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Active sitei236Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processAmino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00034; UER00025

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
Short name:
DAP epimeraseUniRule annotation
Alternative name(s):
PLP-independent amino acid racemaseUniRule annotation
Gene namesi
Name:dapFUniRule annotation
Ordered Locus Names:Acel_1483
OrganismiAcidothermus cellulolyticus (strain ATCC 43068 / 11B)
Taxonomic identifieri351607 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaAcidothermalesAcidothermaceaeAcidothermus
Proteomesi
  • UP000008221 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000776881 – 303Diaminopimelate epimeraseAdd BLAST303

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi351607.Acel_1483

Structurei

3D structure databases

ProteinModelPortaliA0LUZ5
SMRiA0LUZ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni107 – 108Substrate bindingUniRule annotation2
Regioni227 – 228Substrate bindingUniRule annotation2
Regioni237 – 238Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E4Z Bacteria
COG0253 LUCA
HOGENOMiHOG000220467
KOiK01778
OMAiSMCGNGG
OrthoDBiPOG091H01QC

Family and domain databases

HAMAPiMF_00197 DAP_epimerase, 1 hit
InterProiView protein in InterPro
IPR018510 DAP_epimerase_AS
IPR001653 DAP_epimerase_DapF
PANTHERiPTHR31689 PTHR31689, 1 hit
PfamiView protein in Pfam
PF01678 DAP_epimerase, 2 hits
TIGRFAMsiTIGR00652 DapF, 1 hit
PROSITEiView protein in PROSITE
PS01326 DAP_EPIMERASE, 1 hit

Sequencei

Sequence statusi: Complete.

A0LUZ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDVPFLKGH ATRNDFVILP DHDDELELDA ALVRAICDRR RGLGADGILR
60 70 80 90 100
VVAVPADDAP VSSAGATADA AAGRPPQPSA GRPPQPAAAR WFMDYRNADG
110 120 130 140 150
SVAEICGNGI RLFARYLVDA GLEEAGRFLV GTRIGPVPVD VPPAGDVTAW
160 170 180 190 200
LPAPELRGGG RARFDGRMLE GVRVSVGNPH LVCVVDTLAD VDFTRAPVLD
210 220 230 240 250
SDEFPDGANV EVVEIERPGV LRMRVYERGV GETWSCGSGA CAVAAVAEGA
260 270 280 290 300
ERIPRPADGW WRIVVPGGEL RIRFDGDRVA LAGPAVVVAE GRLHLAALEK

SDG
Length:303
Mass (Da):32,002
Last modified:December 12, 2006 - v1
Checksum:i3DBBBFB454EFDFE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000481 Genomic DNA Translation: ABK53255.1

Genome annotation databases

EnsemblBacteriaiABK53255; ABK53255; Acel_1483
KEGGiace:Acel_1483

Similar proteinsi

Entry informationi

Entry nameiDAPF_ACIC1
AccessioniPrimary (citable) accession number: A0LUZ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 12, 2006
Last modified: March 28, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health