Reviewed, UniProtKB/Swiss-Prot A0LUZ1 (LEXA_ACIC1)

Names and origin

Protein names

Recommended name:
    LexA repressor
    EC=3.4.21.88

Gene names

Name:	lexA
Ordered Locus Names:	Acel_1479

Organism

Acidothermus cellulolyticus (strain ATCC 43068 / 11B) [Complete proteome] [HAMAP]

Taxonomic identifier

351607 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Acidothermaceae › Acidothermus

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity. HAMAP MF_00015
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity. HAMAP MF_00015
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	LexA repressor HAMAP MF_00015		PRO_0000322709
Regions
	DNA binding	73 – 93	21	H-T-H motif By similarity
Sites
	Active site	199	1	For autocatalytic cleavage activity By similarity
	Active site	236	1	For autocatalytic cleavage activity By similarity
	Site	164 – 165	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0LUZ1-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 41CC74334316B987	FASTA	275	29,575
	10 20 30 40 50 60 MKRSTPRPAR SQAALTTSSE ESPDRVERGG DGVATVTDFP DGPPDETGLT PRQRRILDVI 70 80 90 100 110 120 RDSVRRRGYP PSMREIGEAV GLTSTSSVAH QLMVLQRKGF LRRDPNRPRA VEIRSAESAV 130 140 150 160 170 180 PDASAGHSPA ADRAPSARRP PRGPSPIDSN PDVVAVPLVG RIAAGGPALA EQLIEDVVPL 190 200 210 220 230 240 PRQLVGEGTL FLLQVKGDSM VDAAICDGDW VVVRQQPVAE NGDIVAAMID GEATVKTFKR 250 260 270 RGAHIWLMPH NPQYEPIPGD EATILGRVVA VLRRL

References

[1]

"Complete sequence of Acidothermus cellulolyticus 11B." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Zharchuk I., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Berry A.M., Adney W.S., Normand P., Leu D., Pujic P., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000481 Genomic DNA. Translation: ABK53251.1.
RefSeq	YP_873237.1.
3D structure databases
SMR	A0LUZ1. Positions 155-274.
ModBase	Search...
Protein-protein interaction databases
STRING	A0LUZ1.
Protein family/group databases
MEROPS	S24.001.
Genome annotation databases
GeneID	4485568.
GenomeReviews	Gene locus Acel_1479 in contig CP000481_GR.
KEGG	ace:Acel_1479.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1974.
HOGENOM	HBG679610.
OMA	KVIGVFR.
PhylomeDB	A0LUZ1.
Family and domain databases
HAMAP	MF_00015. LexA. [Tree]
InterPro	IPR006199. LexA_DNA-bd_dom. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA_cons-reg. IPR019759. Peptidase_S24_S26_cons-reg. IPR015927. Peptidase_S24_S26A/B/C. [Graphical view]
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
TIGRFAMs	TIGR00498. lexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA_ACIC1

Accession

Primary (citable) accession number: A0LUZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	December 12, 2006
Last modified:	February 9, 2010
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)