A0LUN6 (A0LUN6_ACIC1) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase PIRNR PIRNR006337
Short name=MTHase PIRNR PIRNR006337
EC=3.2.1.141 PIRNR PIRNR006337

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase PIRNR PIRNR006337
Maltooligosyl trehalose trehalohydrolase PIRNR PIRNR006337

Gene names

Ordered Locus Names:

Acel_1374

Organism

Acidothermus cellulolyticus (strain ATCC 43068 / 11B) [Complete proteome] [HAMAP]

Taxonomic identifier

351607 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Acidothermaceae › Acidothermus

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan. PIRNR PIRNR006337
Pathway	Glycan biosynthesis; trehalose biosynthesis. PIRNR PIRNR006337
Subcellular location	Cytoplasm By similarity PIRSR PIRSR006337-1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. PIRNR PIRNR006337

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR006337 EMBL ABK53146.1 Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	243	1	Nucleophile By similarity PIRSR PIRSR006337-1
	Active site	280	1	Proton donor By similarity PIRSR PIRSR006337-1
	Site	375	1	Transition state stabilizer By similarity PIRSR PIRSR006337-3

Sequences

	Sequence		Length	Mass (Da)
	A0LUN6 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 5B29F88FC418D356	FASTA	577	64,014
	10 20 30 40 50 60 MRPRVWAPNV SQVELVTFRD PQPAVTPMQR RDDGWWETAD ALSPGDRYSF RLDGNDVLPD 70 80 90 100 110 120 PRSRWQPDGV HGPSGVPDLP PARPWRGRPI VGSVIYELHI GTFTQAGTFD AAVERLDYLV 130 140 150 160 170 180 DLGVDTVEIL PVAAFPGHHG WGYDGVFPFA VYEPYGGPAG MRRFVDACHA KGLAVVLDVV 190 200 210 220 230 240 YNHLGPDGNV LGRFGPYFTD TYRTPWGPAL NVDAAESDDV RRYVIDNALW WLEDVGVDGL 250 260 270 280 290 300 RLDAVHAIVD LSATHILEEL AVNVSRAAQR SGRPLQLIAE SDRNDPRLLT PRGDGGYGLD 310 320 330 340 350 360 AQWSDDFHHA LHVTLTGETS GYYGDFRGPA DVAAALTHGY VYTGQYSPYR RRRHGRPLPP 370 380 390 400 410 420 DTSAHQLLGY AQTHDQVGNR ARGERLCHLV SVERARIAAA LVLTAPFTPM LFMGEEWAAG 430 440 450 460 470 480 TPWQYFTDHQ DPNLARAVRE GRRAEFAAFG WSPDDVPDPQ DPETFHRSIL NWSELADTSH 490 500 510 520 530 540 REMLEWYRTL IALRRAEPAL RDPHFADASW DGATQILRIQ RGDIVVAVNL GDKPMRIALP 550 560 570 VRDVLLSSGD VDATGADLHL PPLAVAIART TQRGEMP

References

[1]

"Complete sequence of Acidothermus cellulolyticus 11B." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Zharchuk I., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Berry A.M., Adney W.S., Normand P., Leu D., Pujic P., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43068 / 11B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000481 Genomic DNA. Translation: ABK53146.1.
RefSeq	YP_873132.1. NC_008578.1.
3D structure databases
ProteinModelPortal	A0LUN6.
ModBase	Search...
Protein-protein interaction databases
STRING	A0LUN6.
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4485862.
GenomeReviews	Gene locus Acel_1374 in contig CP000481_GR.
KEGG	ace:Acel_1374.
PATRIC	20673262. VBIAciCel132453_1459.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG367595.
OMA	EGFVYQG.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR022567. Maltooligo_trehalose_bac_C. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K01236.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02922. CBM_48. 1 hit. PF11941. DUF3459. 1 hit. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A0LUN6_ACIC1

Accession

Primary (citable) accession number: A0LUN6

Entry history

Integrated into UniProtKB/TrEMBL:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)