A0LUJ2 (SYA_ACIC1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Acel_1330

Organism

Acidothermus cellulolyticus (strain ATCC 43068 / 11B) [Complete proteome] [HAMAP]

Taxonomic identifier

351607 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Acidothermaceae › Acidothermus

Protein attributes

Sequence length	886 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_B
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_B.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_B
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 886	886	Alanine--tRNA ligase HAMAP MF_00036_B		PRO_0000347470
Sites
	Metal binding	570	1	Zinc Potential
	Metal binding	574	1	Zinc Potential
	Metal binding	672	1	Zinc Potential
	Metal binding	676	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	A0LUJ2 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: F130E189AD09A27C	FASTA	886	95,438
	10 20 30 40 50 60 MESAEIARRW LAFFEKRDHV VVPSTPLVAD DPELLFVVAG MQPFKPYFRG DAPAPWPRAT 70 80 90 100 110 120 SVQKVLRTPD IDEVGKTTRH ATFFHMCGNF SFGDYFKETA IPLAWELLTT PVADGGYGFA 130 140 150 160 170 180 PDRLWVTVYT DDDEAADIWH RVVGLPVDRI QRRGMADNFW SMGVPGPCGP CSEIYYDRGP 190 200 210 220 230 240 EFGVGGGPVA NEERYLEVWN LVFMQYERGP GGAKDNYPIL GELPAKNIDT GMGLERMAAI 250 260 270 280 290 300 LQGVDNIYEI DTTRPILDKA AELTGQRYGS GGQNDVRLRM VADHIRAITM LVNDGVVPSN 310 320 330 340 350 360 EERGYVLRRL MRRVVRAMRL LGAREPTMHE LVATAIAVFT PQYPELSRNA ERIFAVADGE 370 380 390 400 410 420 EASFFSTLAA GTARFEAAVR EAGGGVLSGE QAFVLHDTYG FPIDLTLEMA AEQGVTVDEE 430 440 450 460 470 480 GFRALMAEQR RRAKEDAERR KTGAADRAAY RAAAELLGRP VEFTGYTERS GEAVVRGLLV 490 500 510 520 530 540 DGAAVPAAHA GQRVEVVLDR TPFYAEGGGQ LPDHGVLEFA AGRIDVDDVQ QPLPGLIVHR 550 560 570 580 590 600 GRVADGEITV GETVLARIDV DRRWAISRSH TATHMVHKAF REFLGDTAAQ AGSENAPGRF 610 620 630 640 650 660 RFDFTNPSAV PPSVLGEVEE RVNDLLLHDL EVTAQIMRQQ EAIASGAIAM FGEKYGDQVR 670 680 690 700 710 720 VISIGDWSRE LCGGTHVPHT GHLGVIKIVS ESSIGAGVRR IEALVGLDAY RYLAREAVLV 730 740 750 760 770 780 SQLAEQLKAP ADELPDRIAG MLARLRDAEK ELEKLRQARL LAEAPRLAAA RVDVGGLAVV 790 800 810 820 830 840 AARVDGDGVD ADGLRLLATD LRQRLGGSAV VVLAGVAGGR PVVVAAVGSE ALARGVKAGE 850 860 870 880 LVGVAAKRLG GGGGGRPDFA QGGGTNPAAV DDAVSAALDA VRAQVG

References

[1]

"Complete sequence of Acidothermus cellulolyticus 11B." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Zharchuk I., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Berry A.M., Adney W.S., Normand P., Leu D., Pujic P., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43068 / 11B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000481 Genomic DNA. Translation: ABK53102.1.
RefSeq	YP_873088.1. NC_008578.1.
3D structure databases
ProteinModelPortal	A0LUJ2.
ModBase	Search...
Protein-protein interaction databases
STRING	A0LUJ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4486383.
GenomeReviews	Gene locus Acel_1330 in contig CP000481_GR.
KEGG	ace:Acel_1330.
PATRIC	20673160. VBIAciCel132453_1412.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0013.
HOGENOM	HBG354397.
OMA	MFTNSGM.
ProtClustDB	PRK00252.
Enzyme and pathway databases
BioCyc	ACEL351607:ACEL_1330-MONOMER.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR023033. Ala_tRNA_synth_euk/bac. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ACIC1

Accession

Primary (citable) accession number: A0LUJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	December 12, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program