Reviewed, UniProtKB/Swiss-Prot A0LUB9 (ASSY_ACIC1)

Names and origin

Protein names

Recommended name:
    Argininosuccinate synthase
    EC=6.3.4.5
Alternative name(s):
    Citrulline--aspartate ligase

Gene names

Name:	argG
Ordered Locus Names:	Acel_1257

Organism

Acidothermus cellulolyticus (strain ATCC 43068 / 11B) [Complete proteome] [HAMAP]

Taxonomic identifier

351607 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Acidothermaceae › Acidothermus

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP MF_00005
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP MF_00005
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP argininosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	Argininosuccinate synthase HAMAP MF_00005		PRO_1000000378
Regions
	Nucleotide binding	8 – 16	9	ATP By similarity
Sites
	Binding site	86	1	Citrulline By similarity
	Binding site	116	1	ATP; via amide nitrogen By similarity
	Binding site	118	1	Aspartate By similarity
	Binding site	122	1	Aspartate By similarity
	Binding site	122	1	Citrulline By similarity
	Binding site	123	1	Aspartate By similarity
	Binding site	126	1	Citrulline By similarity
	Binding site	174	1	Citrulline By similarity
	Binding site	258	1	Citrulline By similarity
	Binding site	270	1	Citrulline By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0LUB9-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: CEC597F355EA3994	FASTA	401	43,539
	10 20 30 40 50 60 MTERVVLAYS GGLDTSVCIG WITEQTGAEV IAVAVDVGQG EDMEVIRKRA LACGAVEAEV 70 80 90 100 110 120 IDARAEFAAD YCVPALRANA LYMGRYPLIS ALSRPLIDKH LVAAAHRHKA TAVAHGSTGK 130 140 150 160 170 180 GNDQVRFEVG IGSLDPELRI LAPVRDSGMT RDRAIAFAEA KGLPIEVTKK SPYSIDANLW 190 200 210 220 230 240 GRAIETGFLE DIWNAPIEEI YAYTADPAVP RPADEVVVSF SDGVPVAIDG RPVSPLEAIT 250 260 270 280 290 300 ELNRRAGAQG VGRIDLVEDR LVGIKSREVY EAPGAVALIT AHQELENVTV ERDLARFKRL 310 320 330 340 350 360 VDQRWAELVY DGLWFSPLKR ALDAFIDTAQ RHVTGDIRLV LHGGRAVVTG RRSPQALYDY 370 380 390 400 RLATYDTGDL FDQSLAKGFV ELWGLPSRTA ARRDLAAGAG E

References

[1]

"Complete sequence of Acidothermus cellulolyticus 11B." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Zharchuk I., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Berry A.M., Adney W.S., Normand P., Leu D., Pujic P., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000481 Genomic DNA. Translation: ABK53029.1.
RefSeq	YP_873015.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0LUB9.
Genome annotation databases
GeneID	4485157.
GenomeReviews	Gene locus Acel_1257 in contig CP000481_GR.
KEGG	ace:Acel_1257.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IAPVREW.
Family and domain databases
HAMAP	MF_00005. [Tree]
InterPro	IPR001518. Arginosuc_synth. IPR018223. Arginosuc_synth_CS. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11587. Arginosuc_synth. 1 hit.
Pfam	PF00764. Arginosuc_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00032. argG. 1 hit.
PROSITE	PS00564. ARGININOSUCCIN_SYN_1. 1 hit. PS00565. ARGININOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASSY_ACIC1

Accession

Primary (citable) accession number: A0LUB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)