Reviewed, UniProtKB/Swiss-Prot A0LUB6 (SYY_ACIC1)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase
      Short name=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	Acel_1254

Organism

Acidothermus cellulolyticus (strain ATCC 43068 / 11B) [Complete proteome] [HAMAP]

Taxonomic identifier

351607 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Acidothermaceae › Acidothermus

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02006
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02006
Subunit structure	Homodimer By similarity. HAMAP MF_02006
Subcellular location	Cytoplasm By similarity HAMAP MF_02006.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Tyrosyl-tRNA synthetase HAMAP MF_02006		PRO_1000088573
Regions
	Domain	358 – 418	61	S4 RNA-binding
	Motif	40 – 49	10	"HIGH" region HAMAP MF_02006
	Motif	234 – 238	5	"KMSKS" region HAMAP MF_02006
Sites
	Binding site	35	1	Tyrosine By similarity
	Binding site	174	1	Tyrosine By similarity
	Binding site	178	1	Tyrosine By similarity
	Binding site	237	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0LUB6-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 7B289F9D1732ADED	FASTA	426	46,247
	10 20 30 40 50 60 MTHLLDDLAW RGLIAQSTDL EALRAAMNTG PITVYCGFDP TAPSLHIGHL VQILTLRRFQ 70 80 90 100 110 120 DAGHRPIALV GGATGLIGDP SGRTTERALN DPDVVAGWAE RIHRQLAPFL DFTGERAGQP 130 140 150 160 170 180 AIAVNNLEWT GRLTAIEFLR DVGKHFRVGR MLAKEVVAAR LRSEQGISYT EFSYQILQSL 190 200 210 220 230 240 DFLELYRRYR CVLQIGGIDQ WGNLTSGIEL IHKVEGVDVH ALATPLVTKA DGTKFGKTAG 250 260 270 280 290 300 GAVWLDPALT SPYAFYQFWI NTDDRDVPAY LRYFSLKNKD ELEALEKDAG RDPAARLGQR 310 320 330 340 350 360 ALAEELTTLV HGATECGKVV AASAALFGRG ELAGVEPETL AAALAEAGLV TMPFDPPPRV 370 380 390 400 410 420 VDALVATGLV ESRSAARRVI SEGGAYVNNE KVTDVDAQID PGRLLHGRWA VIRRGRRAVA GVERAG

References

[1]

"Complete sequence of Acidothermus cellulolyticus 11B." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Zharchuk I., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Berry A.M., Adney W.S., Normand P., Leu D., Pujic P., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000481 Genomic DNA. Translation: ABK53026.1.
RefSeq	YP_873012.1.
3D structure databases
SMR	A0LUB6. Positions 4-424.
ModBase	Search...
Protein-protein interaction databases
STRING	A0LUB6.
Genome annotation databases
GeneID	4485154.
GenomeReviews	Gene locus Acel_1254 in contig CP000481_GR.
KEGG	ace:Acel_1254.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0162.
HOGENOM	HBG288125.
OMA	TFYIGFD.
PhylomeDB	A0LUB6.
Family and domain databases
HAMAP	MF_02006. Tyr_tRNA_synth_type1. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA_bd. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY_ACIC1

Accession

Primary (citable) accession number: A0LUB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 12, 2006
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)