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Protein

L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

Gene

mshC

Organism
Acidothermus cellulolyticus (strain ATCC 43068 / 11B)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins.UniRule annotation

Catalytic activityi

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi43ZincUniRule annotation1
Binding sitei58Cysteinyl adenylateUniRule annotation1
Binding sitei223Cysteinyl adenylateUniRule annotation1
Metal bindingi227ZincUniRule annotation1
Metal bindingi252ZincUniRule annotation1
Binding sitei278Cysteinyl adenylate; via amide nitrogen and carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseUniRule annotation (EC:6.3.1.13UniRule annotation)
Short name:
L-Cys:GlcN-Ins ligaseUniRule annotation
Alternative name(s):
Mycothiol ligaseUniRule annotation
Short name:
MSH ligaseUniRule annotation
Gene namesi
Name:mshCUniRule annotation
Ordered Locus Names:Acel_1172
OrganismiAcidothermus cellulolyticus (strain ATCC 43068 / 11B)
Taxonomic identifieri351607 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaAcidothermalesAcidothermaceaeAcidothermus
Proteomesi
  • UP000008221 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004004261 – 403L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseAdd BLAST403

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi351607.Acel_1172.

Structurei

3D structure databases

ProteinModelPortaliA0LU34.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni43 – 46Cysteinyl adenylate bindingUniRule annotation4
Regioni81 – 83Cysteinyl adenylate bindingUniRule annotation3
Regioni245 – 247Cysteinyl adenylate bindingUniRule annotation3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi45 – 55"HIGH" regionUniRule annotationAdd BLAST11
Motifi284 – 288"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiHIECATI.
OrthoDBiPOG091H004K.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.

Sequencei

Sequence statusi: Complete.

A0LU34-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHAWPAPKIP SLPGRNRLPS LFDTASRRLV TVGSQQGASM YVCGITPYDA
60 70 80 90 100
THLGHAATYV AFDLLVRTWH DAGVTVRYAQ NVTDVDDPLL ERARQVDQPW
110 120 130 140 150
EAIAARETAK FRADMAALRV VPPDRYVGVV ESLPQIIGLI EVLRSRGLTY
160 170 180 190 200
ELDGDQYFAT HAIPDFGAVS HLGRDDMIAL FAARGGDPDR AGKKDPLDAL
210 220 230 240 250
LWRGKRPEEP SWPAPFGRGR PGWHVECAAI ALTHLPLPLD VQGGGADLVF
260 270 280 290 300
PHHDMTAAQA EAATGRRFAR AYVHTGLVAY QGEKMSKSLG NLVFVSDLCA
310 320 330 340 350
AGADPMAVRL ALLDHHYRTE WEWTPRLLDE ATDRLAEWRA AVRRPRGAPG
360 370 380 390 400
DGLLAAVRDR LADDLDAPGA IALIDEWTTQ DGDDPDAPTL VAAMADALLG

VHL
Length:403
Mass (Da):43,892
Last modified:December 12, 2006 - v1
Checksum:i242A3F46A2F81D7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000481 Genomic DNA. Translation: ABK52944.1.
RefSeqiWP_011720007.1. NC_008578.1.

Genome annotation databases

EnsemblBacteriaiABK52944; ABK52944; Acel_1172.
KEGGiace:Acel_1172.
PATRICi20672812. VBIAciCel132453_1242.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000481 Genomic DNA. Translation: ABK52944.1.
RefSeqiWP_011720007.1. NC_008578.1.

3D structure databases

ProteinModelPortaliA0LU34.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi351607.Acel_1172.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK52944; ABK52944; Acel_1172.
KEGGiace:Acel_1172.
PATRICi20672812. VBIAciCel132453_1242.

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiHIECATI.
OrthoDBiPOG091H004K.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHC_ACIC1
AccessioniPrimary (citable) accession number: A0LU34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: December 12, 2006
Last modified: November 2, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.