Reviewed, UniProtKB/Swiss-Prot A0LTM0 (MRAY_ACIC1)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	Acel_1007

Organism

Acidothermus cellulolyticus (strain ATCC 43068 / 11B) [Complete proteome] [HAMAP]

Taxonomic identifier

351607 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Frankineae › Acidothermaceae › Acidothermus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity.
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_1000002928
Regions
	Transmembrane	3 – 23	21	Potential
	Transmembrane	51 – 71	21	Potential
	Transmembrane	80 – 100	21	Potential
	Transmembrane	116 – 136	21	Potential
	Transmembrane	152 – 172	21	Potential
	Transmembrane	186 – 206	21	Potential
	Transmembrane	227 – 247	21	Potential
	Transmembrane	251 – 271	21	Potential
	Transmembrane	276 – 296	21	Potential
	Transmembrane	330 – 350	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	A0LTM0-1 [UniParc]. Last modified December 12, 2006. Version 1. Checksum: 44547EF56E0336D4	FASTA	354	37,496
	10 20 30 40 50 60 MKGILIAASV ALLISLFGTP IAIRFFRERG YGQLIRDDGP TTHHTKRGTP TMGGTVILAS 70 80 90 100 110 120 ALLGYAVANL FTASGPTASG LLVCGLATGL GLVGFLDDFI KIRMQRNLGL RARTKLAGQV 130 140 150 160 170 180 IVAVIFGVLA VQFPNHAGLR PASTHVSFVR DYPSLTLGTI GFVIWALLMV SATSNGVNLT 190 200 210 220 230 240 DGLDGLATGA SAMVFGAFVI ITFWEARNSC LAKAAPGCYH VRDPFDLAIV AAAFAGACAG 250 260 270 280 290 300 FLWWNAAPAR IFMGDTGSLA LGGALAGMAI MSHTEVLLAI LGGLFVVITM SSIIQIASFK 310 320 330 340 350 LTRRRIFKMA PLQHHFELKG WAEVTIVIRF WIIAGLAVAL GLGVFYGEWI TWQG

References

[1]

"Complete sequence of Acidothermus cellulolyticus 11B." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Zharchuk I., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Berry A.M., Adney W.S., Normand P., Leu D., Pujic P., Richardson P. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000481 Genomic DNA. Translation: ABK52780.1.
RefSeq	YP_872766.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0LTM0.
Genome annotation databases
GeneID	4485011.
GenomeReviews	Gene locus Acel_1007 in contig CP000481_GR.
KEGG	ace:Acel_1007.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HHHFEQK.
Family and domain databases
HAMAP	MF_00038. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. PF10555. MraY_sig1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. False negative. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_ACIC1

Accession

Primary (citable) accession number: A0LTM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 12, 2006
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)