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A0LSY4 (SYL_ACIC1) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Acel_0771
OrganismAcidothermus cellulolyticus (strain ATCC 43068 / 11B) [Complete proteome] [HAMAP]
Taxonomic identifier351607 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesFrankineaeAcidothermaceaeAcidothermus

Protein attributes

Sequence length832 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 832832Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334724

Regions

Motif58 – 6811"HIGH" region HAMAP-Rule MF_00049
Motif598 – 6025"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6011ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A0LSY4 [UniParc].

Last modified December 12, 2006. Version 1.
Checksum: 68DA050860DB42E0

FASTA83292,222
        10         20         30         40         50         60 
MAGLGSPLGR LTAVTSEIYD FAAIQARWLP VWTELDPFRA SDDPSDPRER RYMLDMFPYP 

        70         80         90        100        110        120 
SGDLHMGHAE AFAIGDVVAR YWFQRGYNVL HPIGWDAFGL PAENAAIQRN LHPADWTYRN 

       130        140        150        160        170        180 
IETQAASFRN YAISFDWSRR LHTCDPEYYK WTQWLFLRLF ERGLAYRKAS PVNWCPNDQT 

       190        200        210        220        230        240 
VLANEQVVGG TCERCGAQVT KKTLTQWYFR ITEYAQRLLD DMALLEGRWP ERVLTMQRNW 

       250        260        270        280        290        300 
IGRSEGAYVD FTIEGRAEPV TVFTTRPDTL YGATFFVIAA DSPLAAEICA PEQRAAFEAY 

       310        320        330        340        350        360 
VDQVRRLSDI DRLSTERQKT GVFLGRYAVN PVNGERIPVW AADYVLADYG TGAIMAVPAH 

       370        380        390        400        410        420 
DQRDLDFALT YGLPVRVVVD TGEGDPAVTG VATEGDGVHI NSGLIDGTDK AEGIARITRY 

       430        440        450        460        470        480 
LEDIGKGRAG VTYRLRDWLV SRQRFWGAPI PIVHCPGCGE VAVPDQDLPV LLPDLRGADL 

       490        500        510        520        530        540 
APKGISPLAG AADWVQTTCP RCGGSAQRDT DTMDTFVDSS WYYLRYCSPH DPSQPFDVAK 

       550        560        570        580        590        600 
VRQWLPVHQY VGGVEHAILH LLYSRFITKV LHDMGLVDFV EPFSALLNQG QVINQGKAMS 

       610        620        630        640        650        660 
KSLGNGVDLG EQLATYGVDA VRLTMVFAGP PEEDIDWADM NPGALGKFLA RVWRIAGEVT 

       670        680        690        700        710        720 
SPVGAPPKDG DPALRRVTHR TIREVTELVE SFRFNVAVAR VMELANALRK AIDTGPGPAD 

       730        740        750        760        770        780 
PAVREGAEAL AVMLSLFAPY TAEECWARLG HQPTVAKAGW PTPDPELLAQ EEVTCVVQVN 

       790        800        810        820        830 
GKVRERLRVS PSISEEELRA AALAAPAVEK AIDGRPVQRI IVRAPKLVNV VV 

« Hide

References

[1]"Complete sequence of Acidothermus cellulolyticus 11B."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Zharchuk I., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. expand/collapse author list , Berry A.M., Adney W.S., Normand P., Leu D., Pujic P., Richardson P.
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43068 / 11B.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000481 Genomic DNA. Translation: ABK52544.1.
RefSeqYP_872530.1. NC_008578.1.

3D structure databases

ProteinModelPortalA0LSY4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING351607.Acel_0771.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK52544; ABK52544; Acel_0771.
GeneID4485307.
KEGGace:Acel_0771.
PATRIC20671946. VBIAciCel132453_0815.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMALHMGHAE.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycACEL351607:GIXW-788-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_ACIC1
AccessionPrimary (citable) accession number: A0LSY4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 12, 2006
Last modified: April 16, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries